Open Access

Rho GTPases as therapeutic targets in cancer (Review)

  • Authors:
    • G. A. Cardama
    • N. Gonzalez
    • J. Maggio
    • P. Lorenzano Menna
    • D. E. Gomez
  • View Affiliations

  • Published online on: August 9, 2017     https://doi.org/10.3892/ijo.2017.4093
  • Pages:1025-1034
  • Copyright: © Cardama et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Rho GTPases are key molecular switches controlling the transduction of external signals to cytoplasmic and nuclear effectors. In the last few years, the development of genetic and pharmacological tools has allowed a more precise definition of the specific roles of Rho GTPases in cancer. The aim of the present review is to describe the cellular functions regulated by these proteins with focus in deregulated signals present in malignant tumors. Finally, we describe the state of the art in search of different experimental therapeutic strategies with Rho GTPases as molecular targets.

1. Introduction

In the last decades, several signaling pathways have been identified as key molecular regulators of cell behavior, among them the Rho GTPases. The Rho family of GTPases is a family of small (~21 kDa) monomeric G proteins that belong to the Ras superfamily of GTPases. The Ras superfamily of GTPases comprises more than 50 members that share several common features: their molecular weight (18–28 kDa), their C-terminal polyisoprenylation region and the property to bind to and hydrolyze guanine nucleotides (1).

Rho GTPases are key regulators of cytoskeletal structure and dynamics influencing cell adhesion, morphology and progression through the cell cycle. They act as a molecular switch cycling between an inactive GDP-bound and an active GTP-bound state. The Rho GTPases form 8 subfamilies; one subfamily comprises Rac1, Rac2, Rac3 and RhoG; the second subfamily contains Cdc42, TC10 (also known as RhoQ) and TC10-like protein (TCL; also known as RhoJ); a third subfamily includes CHP (also known as RhoV) and WNT1-responsive Cdc42 homologue-1 (WRCH1; also known as RhoU); a fourth subfamily involves RhoH; a fifth subfamily embraces RhoBTB1 and RhoBTB2; a sixth subfamily embodies RhoA, RhoB and RhoC; a seventh subfamily consists of RND1, RND2 and RND3 (also known as RhoE); and the last subfamily is composed by RAP1-interacting factor-1 (RIF; also known as RhoF) and RhoD (2).

2. Structure and function

Up to date three members of the Rho GTPase family have been studied in detail: Cdc42, Rac1 and RhoA. These are all the classical Rho GTPases that cycle between active GTP-bound forms and inactive GDP-bound forms.

The biological activities of Rho family GTPases are controlled by their guanine nucleotide binding states in a process dependent of Mg2+. In most cases, members of the Ras superfamily when they are in their active state, they transmit signals by binding to a specific set of proteins termed effectors, leading to a signaling cascade. The Rho GTPases have functional domains in common, similar to those of Ras. Such domains consist of four regions that take part in the binding and hydrolysis of guanine nucleotides (G1, G3, G4 and G5), a G2 region involved in the interaction with effectors (3) and a terminal CAAX box (being C a cysteine residue, A an aliphatic residue and X any other amino acid) (4). The CAAX box acts as a signal for protein prenylation which involves the addition of C15 (farnesyl) or C20 (geranyl-geranyl), a phenomenon which is catalyzed by protein prenyl-transferases. Following prenylation, additional enzymatic processing including proteolysis and methylation occurs (5). While not as common, some Rho family members need the covalent addition of a palmitate acyl chain to their C-terminal hypervariable domain found immediately adjacent to the CAAX box. Similar to prenylation, palmitoylation plays a pivotal role in directing these Rho family members to membrane-associated states (6). Post-translational modifications do not affect GTPase activity but are essential for their biological function since they control their anchorage to cell membrane, therefore, their localization inside the cell (7).

Rho proteins are highly regulated. There are three types of regulatory proteins: i) GEFs: which stands for guanine-nucleotide exchange factors that catalyze the release of GDP and the subsequent uptake of GTP. ii) GAPs: (also known as GTPase-activating proteins or GTPase-accelerating proteins) are a family of regulatory proteins whose members can bind to activated G proteins and stimulate their GTPase activity, with the result of terminating the signaling event; and iii): GDIs or GDP-dissociation inhibitors which regulate the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them (8).

The small GTPases of the Rho family are master regulators of actin cytoskeleton rearrangements and cell morphology. RhoA can be activated by extracellular ligands (such as lysophosphatidic acid) and its activation results in the assembly of stress fibers and focal adhesion complexes. Rac1 is activated in response to various stimuli including growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF) or insulin, leading to lamellipodia formation and the constitution of a motile cell surface that contains a meshwork of newly polymerized actin filaments known as membrane ruffling or cell ruffling. Rac1 also plays a pivotal role in promoting the formation of nascent focal complexes (9).

Integrin-mediated cell-extracellular matrix adhesion activates Rac1, which directly binds and activates p21-activated kinases (PAKs) and other effectors including phosphatidylinositol-4-phosphate 5-kinase, Nap125, PIR121 and IRSp53, resulting in increased membrane protrusions and actin polymerization. On the other hand, activated Cdc42 phosphorylates PAK1 and PAK2, which in turn lead to filopodia formation (1013). These changes either by themselves or integrated can affect gene expression, cell cycle progression and apoptosis. Although, different cell processes are modulated by Rho GTPases, it is important to highlight that the cellular events are regulated by integrated signaling networks and not by a single signaling cascade. Then, the same stimuli in different cell contexts could produce different responses.

An example of cross talk between Rho GTPases and other regulating pathways is the regulation of Rac1-GAP β2-chimaerin by the protein kinase C (PKC). Rac1-GAPs β2-chimaerins are activated by the lipid second messenger diacylglycerol upon activation of tyrosine kinase receptors. It has been described that epidermal growth factor (EGF) causes rapid phosphorylation of β2-chimaerin via PKCδ retaining it in the cytosol and preventing its translocation to membranes (14). Also, there is evidence of intercommunication between the different Rho GTPases, whereas RhoA and Rac1 regulate each other, then modulating different cell processes (15,16).

3. Main effectors of Rho GTPases

Approximately 1% of the human genome encodes proteins that either regulate or are regulated by interactions with members of the Rho family of small GTPases. The wide variety of biological functions of the different members of the Rho GTPases is due to the binding to different effectors. More than 50 effectors of RhoA, Rac1 and Cdc42 are known including serine-threonine kinases, tyrosine-kinases, lipid-kinases, lipases, oxidases and structural proteins (scaffold proteins) (17).

At least two Rho effectors have been studied in detail: the Rho-associated protein kinases (ROCKs) and the formin mDia1 (mammalian homolog of the Drosophila Diaphanous protein) (18). ROCK1 and ROCK2 share 65% overall homology and 92% homology in the kinase domain (19). Both are serine/threonine kinases that are downstream targets of the small GTPases RhoA, RhoB and RhoC. Structurally they contain a catalytic kinase domain at the N-terminus, a central coiled-coil domain, which includes the Rho-binding domain (RBD) and a C-terminal pleckstrin-homology (PH) domain. ROCKs are involved in diverse cellular activities including actin cytoskeleton organization, cell adhesion and motility, proliferation, apoptosis, remodeling of the extracellular matrix (ECM) and smooth muscle cell contraction. Formins also regulate the actin and microtubule cytoskeleton and are involved in various cellular functions such as cell polarity, cytokinesis, cell migration and serum response factor (SRF) transcriptional activity. Some formins can contain a GTPase-binding domain (GBD) required to bind to Rho GTPases and a C-terminal conserved DRF auto-regulatory domain (Dia-autoregulatory domain or DAD). The GBD domain is a bifunctional auto-inhibitory domain that interacts with and is regulated by activated Rho family members. DAD induces actin filament formation, stabilizes microtubules and activates serum-response mediated transcription (20).

On the other hand, effector proteins of Cdc42 as WASP (Wiskott-Aldrich syndrome protein) and N-WASP are involved in the formation of filopodia. WASP is only expressed in hematopoietic cells. N-WASP, which is ubiquitously expressed, shares around 50% homology with WASP. Both proteins possess several domains: a PH domain that binds phosphatidylinositol (4,5) bisphosphate, a Cdc42-binding (GBD) domain, a proline-rich region, a G-acting binding verprolin homology (V) domain, a domain (C) with homology to the actin-depolymerizing protein cofilin and finally a C-terminal acidic segment. Both WASP and N-WASP induce actin polymerization when overexpressed in fibroblasts. WASP proteins bind directly to acting monomers and activate the Arp2/3 complex; they comprise a core mechanism that directly connects signal transduction pathways to the stimulation of acting polymerization (21).

Furthermore, Rac1 is also able to activate the Arp2/3 complex and this is one of the core regulatory pathways driving membrane protrusion. Activation of WAVE (WASP-like verprolin-homologous protein) complex requires simultaneous interactions with prenylated Rac1-GTP and acidic phospholipids, as well as a specific state of phosphorylation. Together, these signals promote full activation in a highly cooperative process leading to the formation of lamellipodia. Also another major effector of both Rac1 and Cdc42 is PAK1. PAK1 phosphorylates LIM-kinase at threonine 508 within LIM-kinase's activation loop, increasing LIM-kinase-mediated phosphorylation of the actin-regulatory protein cofilin. Activated GTPases can thus regulate actin depolymerization through PAK1 and LIM-kinase modulating microfilaments length (22).

Another process regulated by Rho GTPases is the intracellular organization of microtubules, playing a central role in cell polarity and mitotic spindle assembly (23). The microtubule tip protein CLIP-170 interacts with the Cdc42/Rac1 effector IQGAP and mediates transient capture of microtubules. IQGAP1 is a scaffold protein essential for cellular signaling in response to external cues, linking dynamic microtubules to steer cell migration via interacting with the plus-end tracking protein SKAP (24).

It is important also to point out the role played by stathmin which is crucial for the regulation of the microtubule cytoskeleton. Stathmin interacts with two molecules of dimeric α- and β-tubulin to form a tight ternary complex called the T2S complex. When stathmin sequesters tubulin into the T2S complex, tubulin becomes non-polymerizable. Without tubulin polymerization, there is no microtubule assembly. Stathmin also promotes microtubule disassembly by acting directly on the microtubule ends. Regulation of stathmin is cell cycle-dependent and controlled by the cell's protein kinases in response to specific cell signals. Stathmin phosphorylation increases the concentration of tubulin available in the cytoplasm for microtubule assembly. For cells to assemble the mitotic spindle, stathmin phosphorylation must occur. At cytokinesis, the last phase of the cell cycle, rapid dephosphorization of stathmin occurs to block the cell from entering back into the cell cycle until it is ready (25).

In addition to their cytoskeletal effects, Rho GTPases regulate several signal transduction pathways that lead to alterations in gene expression affecting several transcription factors such as SRF, NF-κB, JNK (c-jun N-terminal kinase) and p38 MAP kinase (17).

Also, Rho GTPases are able to modulate different enzymatic activities. For instance, Rac1 plays a crucial role in activation of Nox family NADPH oxidases in enzymes dedicated to production of reactive oxygen species such as superoxide. The phagocyte oxidase Nox2, crucial for microbicidal activity during phagocytosis, is activated in a Rac1-dependent manner. Rac1 in the GTP-bound form directly binds to the oxidase activator p67 (PHOX), which in turn interacts with NOX2, leading to superoxide production (26,27).

Furthermore, Rho GTPases have also been shown to regulate cell cycle entry and cell cycle progression, in particular by regulating expression of a number of genes involved in G1/S transition, such as cyclin D1 or p21waf1. Rho GTPases are also critically involved in mitosis. Indeed, at mitosis onset, RhoA activity increases and the resulting activation of its effector ROCK, mediates cortical retraction during mitotic cell rounding. During early mitosis, depending on the cell type, either the GEF-H1/RhoA/mDia1 or the Ect2/Cdc42/mDia3 pathways are needed for spindle assembly and attachment of microtubules to kinetochores. Later in mitosis, Rho GTPases are directly involved in cytokinesis by regulating the actin and myosin contractile ring, which eventually forms the cleavage furrow to separate daughter cells (28).

It is important to highlight that cells perceive their microenvironment not only through soluble signals but also through physical and mechanical cues, such as ECM stiffness or confined adhesiveness. By mechano-transduction systems, cells translate these stimuli into biochemical signals controlling multiple aspects of cell behavior, but how rigidity sensing is ultimately linked to activity of nuclear transcription factors just started to be understood. Downstream events involving Rho GTPases in response to cytoskeleton tensioning include activation of YAP (yes-associated protein) and TAZ (transcriptional coactivator with PDZ binding motif) transcriptional regulators as nuclear relays of mechanical signals exerted by ECM rigidity and cell shape (29).

4. Role of Rho GTPases in cancer

In every physiological process each signaling element has an exquisite temporal and spatial regulation; at the same time deregulated expression leads to the development of many diseases among them cancer, the focal point of the present study (30). As mentioned, Rho GTPases through a series of complex biochemical networks control some of the most fundamental processes of the cell. Mutations or alterations of this complex signaling system could lead to cancer development. Traditionally, overactivation of the Rho GTPase pathways has been associated with malignant transformation. Recently, it has been described in a gain-of-function mutation in the rac1 gene in sun-exposed melanomas, although rac1 has been rarely found mutated in other human cancers (3032). Rac1 upregulation is mostly due to alterations of its regulatory proteins. Notably, GEF activation is the most common mechanism for signal-mediated GTPase activation. This activation is commonly driven by aberrant signaling from growth factor receptors (RTKs) and GPCRs (G protein coupled receptors) and upregulation or mutation of GEFs (33). In this regard, many GEFs present a relevant role in cancer such as Ect2, Tiam1, Vav family, P-Rex1, DOCK family among others (3437).

First evidence of malignant transformation by Rho GTPases was provided by experiments where constitutively activated Rac1, and in a lesser extent RhoA, induced malignant transformation in fibroblasts and tumorigenicity in athymic mice (38,39). In line with this idea, Rac1 is implicated in epithelial-mesenchymal transition and in mesenchymal-epithelial transition (4043). Currently, alterations in the Rho GTPase pathways can be found in many cancer types. Among them we can mention malignant tumors of pancreas, breast, skin, prostate, testicle, colon, liver, lung, stomach as well as in leukemia, osteosarcoma, neuroblastoma, glioblastoma and head and neck cancer (4447). Although Rho GTPase overexpression is commonly observed in cancer, downregulation of some Rho family members has also been linked to cancer (48).

Loss of cell polarity is a hallmark of cancer, with the loss of epithelial cell polarity being common among carcinoma cells (49,50). For cells to keep their polarity, cell-to-cell contact must be maintained, being Rac1, RhoA and Cdc42 indispensable for keeping a normal state (51). Regarding cell motility the Rho/ROCK pathway plays a central role in the acquisition of migratory and invasive properties in tumor cells (52), since they are implicated in the remodeling of the actin cytoskeleton, in intracellular adhesion mediated by cadherins and in the remodeling of the extracellular matrix (ECM) primarily by the release of matrix metalloproteinases (MMP), mainly MMP-9 (53).

It has been found that Rac1 is a key protein in the invasive process of pediatric medulloblastoma (54). In models of glioblastoma multiforme Rac1, Rac3 and some GEFs mediate the migratory and invasive capacity of these tumors (36,55,56).

Once a tumor is established, neovascularization of the pathological tissue is essential (57). In vitro and in vivo studies have identified Rho proteins as essential in signaling for vascular endothelial growth factor (VEGF)-dependent capillary formation (58). Regarding the regulatory proteins of the GTPases involved in cancer, one of the most studied examples is the Rac1-GEF Tiam1 (T-cell lymphoma invasion and metastasis 1). Although GTPases are not considered oncogenes, Tiam1 was confirmed as one, since it is implicated in the oncogenic transformation of fibroblasts (59,60). The Tiam1 gene was identified by insertional mutagenesis in a model of T cell lymphoma, carrying out an in vitro selection of highly invasive clones. In this way it was established that Tiam1 increases the invasive capacity and the metastatic potential of tumor cells (59). Additionally, Tiam1 levels are increased and could be correlated with the prognosis of human prostate carcinomas, nasopharyngeal carcinomas and with the degree of progression of breast tumors (61).

In line with this idea, Vav1, another Rac1 GEF, is over-expressed in pancreatic adenocarcinoma and metastatic melanoma (62).

Also, the expression levels of Rac1-GAP β2-chimaerin are significantly diminished in mammary tumors (63). Furthermore, it has been shown that overexpression of β2-chimaerin in a murine mammary carcinoma model produces the reduction of tumor growth rate and its invasive and metastatic capacity (64).

Another hallmark exhibited by cancer cells is the limitless replicative potential that is mainly due to the activity of telomerase. This holoenzyme maintains telomere length, adding TTAGGG repetitions at the end of chromosomes in each cell division (65). In addition to this function, there are extratelomeric roles of telomerase that are involved in cancer promoting events (66). It has been shown that telomerase reconstitution increased fibroblast migration through activation of CXCL12/CXCR4 axis and Rho family proteins (67).

Yeh et al (68) demonstrated that Cdc42/Rac1 participates in the control of telomerase activity in human nasopharyngeal cancer cells (NPC-076). They investigated the effects of inhibiting Cdc42 and Rac1 on the telomerase activity on cells. Treatment of NPC-076 cells with antisense oligonucleotides against Cdc42 or Rac1 produced an inhibition of telomerase activity. Similarly, transient expression of dominant-negative mutants of Cdc42 or Rac1, also produced an inhibition of telomerase activity in NPC-076 cells. This inhibition of telomerase activity was not associated with a transcriptional downregulation of hTERT, the key regulator of telomerase (69). This suggests that Cdc42/Rac1 participate in the post-transcriptional control of telomerase activity in NPC-076 cells. It also was founded that RAC3 overexpression is required to maintain telomerase activity (70). Further analysis suggested that exogenous expression of hTERT may promote invasiveness and metastasis through upregulation of MMP9 and RhoC (71).

Since telomerase is a key target against cancer, many molecules have been developed against it. Lately, it has been postulated that telomerase regulation could be better than direct inhibition itself (72). It remains to know if Rho GTPases could exert this action in a more effective way that the ones that are known so far.

Stathmin is also a protein of clinical relevance in cancer. Stathmin's role in regulation of the cell cycle causes it to be oncoprotein 18 (op18). Op18/stathmin can cause uncontrolled cell proliferation when mutated and not functioning properly. If stathmin is unable to bind to tubulin, it allows for constant microtubule assembly and therefore constant mitotic spindle assembly. With no regulation of the mitotic spindle, the cell cycle is capable of cycling uncontrollably resulting in the unregulated cell growth characteristic of cancer cells (73).

Among the many different effectors of Rho GTPases, four of them are directly related with malignant transformation mediated by Rho proteins. First, we can mention the WASP family, effectors of Rac1 and Cdc42, related with actin cytoskeleton rearrangements, therefore associated to cell migration, filopodia, podosomes as well as membrane ruffles regulation. Another described effector is IQGAP, which by its interaction with Rac1 and Cdc42 is implicated in membrane ruffles and in the cell-cell unions mediated by E-cadherin (74,75).

Thirdly, we should mention that the role of PAK in cancer has been widely described. They have more than 40 possible different substrates and mediate biological processes such as cell proliferation, cell motility and survival, angiogenesis and substrate-independent growth, among others (76). It has been described that by Rac1 activation, PAK1 is able to activate PKCγ, a necessary event for the interaction with the actin-bundling protein fascin, mediating cell migration in a model of human colon carcinoma (77).

Finally, ROCK1 directly interacts with and stabilizes the oncogene c-Myc protein leading to the increased oncomir miR-17-92 cluster expression in breast cancer and prostate cancer (78); ROCK family members are direct effectors also of RhoA (79).

It is important to highlight that Rac1 may also drive anti-tumorigenic effects (80). Recently Zandvakili et al (81) reviewed the role of Rho GTPases with potential tumor suppressing roles, however, most of that data emerged from cancer genomic studies. In those studies the concept of mutation penetrance and expressivity have been not sufficiently analyzed to be conclusive about the subject (82). In any case, the redundant function among Rho GTPases, their many regulators and effectors added to the interplays of feedback signaling loops may explain such contradictions.

Although we just gave an overview on how different Rho-GTPases and their associated proteins may play a defined role in cancer, it should be taken into account that given the overlap between its physiological and pathological functions as well as its often contrasting effects in cellular processes, these different molecules could be, at times, playing a similar role.

5. Rho GTPases as therapeutic targets in cancer

The substantial body of evidence that relates Rho GTPases with cancer has made them an attractive therapeutic target at the molecular level. With an incredible level of complexity and as more knowledge was being incorporated to the true nature of Rho GTPases, many groups started the search of inhibitors that could influence negatively the characteristics of tumor cell growth. Some strategies used to develop inhibitors are shown in Fig. 1 and described below.

Targeting Rho-GEF interactions

Disrupting Rho interactions with specific GEFs represents an attractive therapeutic avenue. The first compound to be characterized was NSC23766 (83). It acts by inhibiting Rac1 activation by blocking the association of Rac1 and Tiam1 as well as Rac1 and Trio, another GEF. NSC23766 has been shown to inhibit pro-tumorigenic effects in a number of cancer models (84). NSC23766 suppressed the cell migration and growth of gefitinib-resistant non-small cell lung cancer (NSCLC) cells (85). Regarding breast cancer, treatment with trastuzumab improves outcomes for ErbB2-positive tumors; many patients who achieve an initial response to trastuzumab subsequently acquire resistance. Dokmanovic et al (86) demonstrated that NSC23766 restores trastuzumab-mediated endocytic downregulation of ErbB2 in vitro. Also, NSC23766 enhanced the antineoplastic effects of erlotinib in glioblastoma cell lines (87). Despite the promising results associated with NSC23766, this compound lacks the efficacy required for clinical purposes. Optimization of NSC23766 led to the identification of a potent Rac1 inhibitor, EHop-016 which functions through interfering Rac1-Vav2 binding and has been shown to suppress Rac1 cell migration of cancer cells (88).

Also by virtual screening, we identified in our laboratory a compound able to interfere with Rac1-GEF binding called ZINC69391. Further in vitro validation demonstrated that the compound interferes with Rac1-Tiam1 binding (89). Inhibition of Rac1 by ZINC69391 was also associated with efficient inhibition of cell cycle progression, cell proliferation and migration in highly metastatic breast cancer cell lines. Even more important in vivo use of ZINC69391 significantly reduced cancer lung metastasis in a breast cancer metastasis mouse model. Also, using ZINC69391 as a lead compound we identified a more potent analogue, 1A-116 also with in vitro and in vivo antimetastatic properties. Further analysis of 1A-116 indicated that it exerts its effects through interfering with Rac1-P-Rex1 binding and suppressing Rac1 activation. As known, one of the signs of tumor progression in breast and prostate cancer is the transition from being hormone-dependent to hormone-independent, with the consequent resistance to antihormonal treatments; therefore, we also analyzed the effect of 1A-116 in tamoxifen resistant breast cancer cells. Deregulation of survival signaling pathways play a key role in tamoxifen resistance, being upregulation of Rac1-PAK1 signaling pathway one of the most important. We developed a breast cancer cell model (MCF7::C1199) having Rac1 enhanced activity. These cells not only showed distinctive features of Rac1-regulated processes as increased migration and proliferation rates, but also showed that upregulation of Rac1 activity triggered a hormonal-independent and tamoxifen resistant phenotype. We also demonstrated that PAK1 activity raises in response to tamoxifen, increasing phosphorylation levels of estrogen receptor at Ser305, a key phosphorylation site involved in tamoxifen resistance. 1A-116 effectively restored tamoxifen anti-proliferative effects, switched off PAK1 activity and decreased estrogen receptor phospho-Ser305 levels (90). Furthermore, PAK and the GEF AND-34 could be involved in the hormone-independence of mammary tumors (91,92). This data support the use of antihormonal therapies combined with Rac1 inhibitors in the above-mentioned tumors.

We also analyzed the effect of both the parental molecule and the derived 1A-116 in malignant gliomas, whereas deregulation of small GTPases signaling, in particular Rac1 activity, plays a key role in their invasive phenotype. We reported that ZINC69391 on human glioma cell lines was able to interfere with the interaction of Rac1 with Dock180, a relevant Rac1 activator in glioma invasion, and to reduce Rac1-GTP levels. Pak1 was also downregulated upon ZINC69391 treatment. ZINC69391 reduced cell proliferation, affected cell migration and invasion in vitro; interfering with actin cytoskeleton, arrested cells in G1 phase and triggered apoptosis in glioma cells. We also evaluated the effect of 1A-116, which showed an even greater antiproliferative and anti-invasive activity on glioma cells (93). Currently, we are carrying out preclinical testing in animal models of glioma with promising results (data not shown). Rho GTPases are also associated with increased invasive potential of glioblastomas after radiotherapy. The possibility has been proposed of inhibiting pharmacologically the Rac1 pathways with the aim of increasing the therapeutic efficacy of radiotherapy (9496).

Additionally, CASIN has been described as a Cdc42 inhibitor that disrupts its interaction of its GEF intersectin (97). More selective Cdc42 inhibitors as ML141 (98) and ZCL278 (99) have been published and are under evaluation.

Other approaches include the identification by virtual screening of Rhosin, which binds to the surface of RhoA by Trp58 and blocks Rho-A-mediated cytoskeletal activity and invasiveness of breast cancer cells (100).

Targeting Rho-nucleotide interactions

Another important class of inhibitors includes compounds that specifically interfere with Rac1 nucleotide binding. Few drugs were developed in this area, and even less showed an effective action. The best example in this group is the well characterized EHT 1864 which displaces bound nucleotides resulting in an inert and inactive Rac1 state, which prevents GEF-mediated nucleotide exchange as well as Rac1 binding to downstream effectors, effectively blocking transformation mediated by constitutively active Rac1 (101).

Recently, Arnst et al (102) demonstrated two new compounds, referred in their publication as #1 and #6, that were design to target the nucleotide-binding site of Rac1 although, were able to block active Rac1 from binding to its effector PAK1, following EGF-induced Rac1 activation in a dose-dependent manner, they showed no inhibition of Cdc42 or RhoA. Functional studies indicated that both compounds reduced cell proliferation and migration in a dose-dependent manner in multiple pancreatic cancer cell lines at micromolar concentrations.

Targeting Rho spatial regulation

Since Rho proteins require the post-translational addition of lipid residues in their carboxy-terminal region, a number of compounds have been developed with the intention of preventing such modification. In this group we can find farnesyltransferase and geranylgeranyl-transferase inhibitors. These compounds were demonstrated to have a high antitumor effect and several of them are in clinical trials (103).

Statins is a class of inhibitors that by inhibiting the HMG CoA reductase pathway simultaneously inhibit the production of both cholesterol and specific prenylated proteins, diminishing cholesterol levels and the lipid modifications needed by Rho GTPases. A possible disadvantage of isoprenylation inhibition is the low selectivity found among the different GTPases. However, clinical trials indicate that statins do affect tumor progression (104,105). Recent data showed a prenylation-independent mode of action in which the protective effects of statins were attributed to the degradation of the nuclear pool of Rac1 (106) implicated in regulating cell cycle progression (107) and actin polymerization in the nucleus (108). Therefore, by stimulating nuclear Rac1 degradation, the protective effects of statins might also be a direct consequence of suppressing nuclear Rac1. For example, an analysis of nuclear Rac1 expression in cervical pre-malignant biopsies indicated increased expression in biopsies with low-grade squamous intraepithelial lesions (SIL) and high grade SIL. Also, nuclear Rac1 was observed in cervical cancer cell lines but not in their normal counterparts suggesting a role of nuclear Rac1 in disease progression (109). It was also shown that Rac1 nuclear accumulation mediates tumor cell invasion due to increased RhoA signaling in the cytoplasm.

Other compounds which interfere with spatial regulation are GGTase I inhibitors (GGTs) which inhibit protein prenylation. Inhibition of GGTase I in a mouse model of pancreatic cancer was associated with reduced tumor growth in relation with the inhibition of protein geranyl-geranylation (110). Similar effects were found in a mouse model of lung cancer (111). GGTI-2418 has entered phase I clinical trials, but results have not been released yet (112). A number of inhibitors of palmitoylation have been developed and can be found in the study of Draper et al (113). However, the therapeutic potential of these compounds is yet to be demonstrated.

Targeting specific Rho downstream effectors

Other strategies used has been the inhibition of the effector proteins. Both ROCK inhibitors Y-27632 and fasudil bind the ATP-binding pocket of ROCK1 and ROCK2 and inhibit serine-threonine kinase activity (114,115). They demonstrated a significant beneficial effect in many types of malignancies (116). Furthermore, it has been reported that the use of Y-27632 could diminish the neurotoxicity caused by antitumor drugs such as cisplatin (117). Fasudil was approved in Japan in 1995 for the prevention and treatment of cerebral vasospasm after surgery in subarachnoid hemorrhagic patients (118). Other competitive inhibitor of ROCK, Wf-536 was demonstrated to be an inhibitor of angiogenesis, tumor growth and metastasis in vivo (119).

Because these ROCK inhibitors are non-isoform specific also inhibit other serine/threonine kinases such as PKA and PKC at higher concentrations, so it may cause off-target effects (120). Efforts have been recently dedicated to the development of ROCK isoform selective inhibitors and several compounds have been reported (121). Despite the numerous ROCK inhibitors developed, there is only one reported clinical trial using ROCK inhibitors in cancer treatment: AT13148 in phase I initiated in 2012 for the treatment of advanced solid tumors (122).

Targeting RhoGTPases by other mechanisms

Azathioprine and its metabolite 6-Mercaptopurine are widely used as immunosuppressing drugs. Its mechanism of action was elucidated, proposing Rac1 as a specific target in T lymphocytes (123). Later it was found that the drug and its metabolite were able to diminish the active Rac1 levels in a model of murine mammary carcinoma in vitro (124). Recently, the phytochemical agent rocaglamide, which belongs to the family of flavaglines, was found to inhibit the activities of Rho, Cdc42 and Rac1 and that it could represent a new class of anticancer drugs (125).

6. Concluding remarks

The redundancy and plasticity of the different pathways allow tumor cells to adapt and to overcome different difficult conditions either of therapeutic or environmental origin, implying that therapies with a unique highly specific target not always reach the levels of efficacy that are desirable. That suggests that different concomitant therapeutic strategies may be needed (126). An option is to combine drugs that modulate multiple signaling pathways. The most effective combinations may include agents that inhibit survival signals in multiple signal transduction pathways.

Targeting Rho GTPases represent a very interesting therapeutic opportunity. As shown, several inhibitors have been developed. While some of these compounds show promising results, it is evident that more potent inhibitors are yet to be identified. However, advancements in rational drug design together with our expanding knowledge of the mechanisms involved in regulating Rho GTPases signaling pave the way toward the development of highly specific and context dependent Rho-GTPase inhibitors.

Acknowledgments

Georgina Cardama is a D-TEC Fellow from ANPCyT, Nazareno Gonzalez is a Conicet Fellow and Julian Maggio is an INC fellow. Pablo Lorenzano Menna and Daniel Gomez are members of Conicet. The present study has been financed by the Universidad Nacional de Quilmes, ANPCyT and CONICET.

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October 2017
Volume 51 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

2016 Impact Factor: 3.079
Ranked #33/217 Oncology
(total number of cites)

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APA
Cardama, G.A., Gonzalez, N., Maggio, J., Menna, P.L., & Gomez, D.E. (2017). Rho GTPases as therapeutic targets in cancer (Review). International Journal of Oncology, 51, 1025-1034. https://doi.org/10.3892/ijo.2017.4093
MLA
Cardama, G. A., Gonzalez, N., Maggio, J., Menna, P. L., Gomez, D. E."Rho GTPases as therapeutic targets in cancer (Review)". International Journal of Oncology 51.4 (2017): 1025-1034.
Chicago
Cardama, G. A., Gonzalez, N., Maggio, J., Menna, P. L., Gomez, D. E."Rho GTPases as therapeutic targets in cancer (Review)". International Journal of Oncology 51, no. 4 (2017): 1025-1034. https://doi.org/10.3892/ijo.2017.4093