Identification of natural antisense transcripts involved in human colorectal cancer development

  • Authors:
    • Keisuke Kohno
    • Mitsuru Chiba
    • Soichiro Murata
    • Sugiru Pak
    • Kentaro Nagai
    • Masayoshi Yamamoto
    • Kazuhiko Yanagisawa
    • Akihiko Kobayashi
    • Hiroshi Yasue
    • Nobuhiro Ohkohchi
  • View Affiliations

  • Published online on: December 1, 2010     https://doi.org/10.3892/ijo_00000794
  • Pages: 1425-1432
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Natural antisense transcripts (NATs) constitute a class of non-coding RNAs that have emerged as important regulators of gene expression. However, involvement of NATs in colorectal cancer (CRC) development has not been reported to date. In the present study, the up- and down-regulation of NATs were investigated in human CRC for their possible involvement in CRC development. Total RNAs isolated from 51 CRC tissues, 9 corresponding non-cancerous tissues and 19 liver metastatic tissues from surgically resected samples were subjected to expression analysis using a custom-microarray containing human sense/antisense probes for ca. 21,000 genes. Comparing CRC tissues with non-cancerous tissues, we identified 415 NATs differentially expressed in CRC and non-cancerous tissues to a significant degree (p<0.001, fold change >4.0 or ≤4.0). When a hierarchical clustering was performed on CRC and non-cancerous samples using these 415 NATs, the samples were separately clustered. Principal component analysis with the same NATs showed clear separation of CRC and non-cancerous samples using the first two principal components (PC1, 80%; PC2, 10%). To validate the expression results obtained from the microarray, the expressions of the 3 selected NATs were examined by strand-specific RT-qPCR, revealing that these expression profiles were consistent with those obtained from microarray analysis. In addition, the NAT expression patterns were found to be different between primary tumors with liver metastasis and those without liver metastasis. In conclusion, these findings taken together indicated that NATs indentified in the present study would be involved in CRC development as well as possibly in its metastasis.

Related Articles

Journal Cover

December 2010
Volume 37 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Kohno K, Chiba M, Murata S, Pak S, Nagai K, Yamamoto M, Yanagisawa K, Kobayashi A, Yasue H, Ohkohchi N, Ohkohchi N, et al: Identification of natural antisense transcripts involved in human colorectal cancer development. Int J Oncol 37: 1425-1432, 2010.
APA
Kohno, K., Chiba, M., Murata, S., Pak, S., Nagai, K., Yamamoto, M. ... Ohkohchi, N. (2010). Identification of natural antisense transcripts involved in human colorectal cancer development. International Journal of Oncology, 37, 1425-1432. https://doi.org/10.3892/ijo_00000794
MLA
Kohno, K., Chiba, M., Murata, S., Pak, S., Nagai, K., Yamamoto, M., Yanagisawa, K., Kobayashi, A., Yasue, H., Ohkohchi, N."Identification of natural antisense transcripts involved in human colorectal cancer development". International Journal of Oncology 37.6 (2010): 1425-1432.
Chicago
Kohno, K., Chiba, M., Murata, S., Pak, S., Nagai, K., Yamamoto, M., Yanagisawa, K., Kobayashi, A., Yasue, H., Ohkohchi, N."Identification of natural antisense transcripts involved in human colorectal cancer development". International Journal of Oncology 37, no. 6 (2010): 1425-1432. https://doi.org/10.3892/ijo_00000794