LncRNAs and cancer (Review)
- Authors:
- Published online on: June 23, 2016 https://doi.org/10.3892/ol.2016.4770
- Pages: 1233-1239
Abstract
Introduction
In the human genome, ≤2% of protein-coding genes are stably transcribed; the remaining RNAs exhibit no protein-coding function. These non-coding RNAs (ncRNAs) include small interfering RNAs (siRNAs), microRNAs (miRNAs), PIWI-interacting RNAs and long ncRNAs (lncRNAs) (1). LncRNAs are a group of non-coding RNAs composed of >200 nucleotides (2). Originally, lncRNAs were considered to be the product of evolutionary junk or transcriptional noise, however, lncRNAs have been demonstrated to exhibit a variety of functions, including chromatin remodeling, mRNA splicing and editing and transcriptional regulation. LcnRNAs may upregulate or downregulate gene expression both transcriptionally and post-transcriptionally. LncRNAs exert a key role in numerous cellular processes, including cell growth, apoptosis and carcinogenesis (3,4) and thus, they are implicated in the progression of various types of human cancer (5). In the present review, the most important and well-known lncRNAs in cancers are discussed and their known and possible molecular functions are outlined briefly, as well as their potential application as therapeutic targets or biomarkers.
Structure and function of lncRNAs
Structure of lncRNAs
LncRNAs are endogenous RNA molecules, which contain 200-100,000 nucleotides. Approximately 7,000–23,000 lncRNAs have been identified in vivo, which is significantly higher than the number of protein-coding genes that have been found. LncRNAs exhibit an mRNA-like structure with a poly (A) tail in certain cases. The promoters of lncRNAs may also bind to transcription factors (TFs), such as Oct3/4 and Nanog.
Based on their location with respect to protein-coding genes, lncRNAs may be classified into the following five groups: i) Intergenic lncRNAs (lincRNA): Located between two protein-coding genes; ii) intronic lncRNAs: Transcribed from the introns of protein-coding genes; iii) overlapping lncRNAs: Contain a coding gene within an intron on the same strand; iv) antisense lncRNAs: Transcribed from the opposite of the protein-coding strand; v) processed lncRNAs: Do not contain an open reading frame (ORF) (6).
Function of lncRNAs
LncRNAs lack a significant ORF and protein-coding function, and are involved in the regulation of gene expression at the epigenetic, transcriptional and post-transcriptional levels in the form of RNA (7). LncRNAs regulate gene expression and chromatin structure in the following ways: i) Decoy effect: Binding to other RNAs and proteins to alter their functions; ii) scaffold effect: Connecting chromatin modified proteins and DNA regions to form signal connections; iii) post-transcriptional effect: Forming RNA dimers with mRNA sequences to block transcription-associated sites, subsequently regulating the stability, cleavage and translation of protein-coding genes (6). Wilusz et al (8) reported that lncRNAs exhibit the following effects: i) Targeting proteins to specific genomic loci to affect transcription patterns; ii) modulating the activity of protein-binding partners; iii) acting as precursors for small RNAs; iv) affecting the processing of other RNAs; v) forming nucleic acids and protein complexes with protein as structural RNAs. In general, lncRNAs regulate gene expression by altering chromatin structure, silencing or activating a gene or a gene family and in certain cases whole chromosomes via cis- or trans-methods (direct regulation of an adjacent gene or indirect regulation via a gene product, respectively).
LncRNA databases
As the number of identified lncRNAs has increased, databases for lncRNAs have been established. These databases include existing lncRNAs and information regarding their known molecular/cellular function, revealing associations between lncRNAs and other RNAs. LncRNA information is also combined with other proteomic and genomic information. Key lncRNA databases are listed in Table I (9).
Cancer-related lncRNAs
A significant association between cancer and lncRNAs has been identified as a result of disease state studies, which have investigated the function of lncRNAs in development and cellular differentiation (10).
To investigate the association between a specific lncRNA and a certain type of cancer, the expression levels of lncRNA in adjacent normal tissues and cancer tissues, as well as normal and cancer cell lines must be compared by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). In vitro and in vivo assays may also be performed to identify the lncRNA's involvement in tumor progression, including cell proliferation, migration, invasion, apoptosis and cell cycle assays. Statistical analysis is also required to investigate the association between lncRNA expression levels and the overall survival rates of cancer patients. The primary cancer-associated lncRNAs are discussed below and listed in Table II.
Digestive system cancers
Taurine-upregulated gene 1 (TUG1) is significantly overexpressed in esophageal squamous cell carcinoma (ESCC) tissues, and silencing TUG1 in vitro using siRNA inhibits the proliferation and migration of ESCC cells and blocks the progression of the cell cycle (11). Furthermore, high TUG1 expression is associated with a family history of ESCC and upper segmental esophageal cancer occurring between the esophageal inlet and upper edge of the manubrium (11). Linc-POU class 3 homeobox 3 (POU3F3) has been characterized as a highly conserved functional transcription regulator in ESCC, which promotes the methylation of POU3F3 by interacting with EZH2 (12,13).
LINC00152 may also be identified in the plasma of cancer patients, and the levels of plasma LINC00152 have been shown to be significantly elevated in gastric cancer (GC) patients (14). Plasma LINC00152 is protected by exosomes and may exist stably in blood, suggesting it may present a potential novel blood-based biomarker for the diagnosis of GC (14). Gastric adenocarcinoma predictive long intergenic noncoding RNA (GAPLINC) regulates CD44-dependent cell invasiveness and is associated with a poor prognosis of GC. GAPLINC regulates CD44 as a molecular decoy for miR211-3p, a miRNA that targets CD44 and GAPLINC (15). LncRNA plasma-cytoma variant translocation 1 (PVT1) expression has been found to correlate with lymph node invasion of GC and is highly expressed in paclitaxel-resistant SGC7901 cells (16). In addition, increased expression of HOX antisense intergenic RNA (HOTAIR) is observed in gastric cardia adenocarcinoma (GCA) tissues when compared with normal tissues (17). HOTAIR expression is also associated with poor survival rates in GCA patients. The single nucleotide polymorphism, rs12826786, may increase the risk of developing GCA by influencing the expression of HOTAIR (17). FENDRR is an lncRNA that binds to polycomb repressive complex 2 (PRC2) to epigenetically regulate the expression of its target gene. The FENDRR gene is 3,099 nucleotides in length and is located at chr3q13.31, consisting of four exons. FENDRR suppresses tumor invasion and lymphatic metastasis in GC via the downregulation of fibronectin 1 and matrix metalloproteinase (MMP)2/MMP9 expression (18). Furthermore, lncRNA AC138128.1 may present a novel biomarker for predicting GC development (19).
HOTAIR, which is encoded in the antisense direction from the HOXC gene cluster and induces transcriptional silencing of HOXD genes through interaction and recruitment of the PRC2 (20), has been shown to be involved in tumor pathogenesis, acting as a promoter in the carcinogenesis of colorectal cancer. Furthermore, it is predictive of poor prognosis in colorectal cancer (20). Recently, a two-stage case-control study revealed that patients with the rs7958904 CC genotype exhibited a significantly decreased risk of both Dukes' stage I and II colorectal cancer compared with those exhibiting the GG genotype, indicating that genetic variations in the HOTAIR gene are associated with the risk of colorectal cancer (21). An additional study indicated that HOTAIR and lncRNA-422 may be involved in carcinogenesis by regulating protein-coding genes involved in specific biological processes associated with colorectal cancer (22). Furthermore, HOTAIR and lncRNA-422 are upregulated when KRAS is overexpressed, but downregulated when Janus-kinase 2 or TANK-binding kinase 1 are knocked out and when platelet-derived growth factor is overexpressed (22). Cancer susceptibility candidate 2 (CASC2) and growth arrest-specific transcript 5 (GAS5) are known tumor suppressors in colorectal cancer (23,24). Colony formation assays have shown that the expression levels of R05532, NR_015441 and NR_033374 positively correlate with radiation resistance, indicating that these lncRNAs may be present predictive markers of radiosensitivity of colorectal cancer (25).
The lincRNA-ubiquitin-fold modifier conjugating enzyme 1, whose stability is reduced by miRNA 34a, promotes proliferation, cell cycle progression and inhibits apoptosis in hepatocellular carcinoma (HCC) cells. Its expression is associated with tumor size, Barcelona clinic liver cancer stage and patient outcome (26). It also regulates β-catenin expression in HCC cells by interacting directly with the mRNA stabilizing protein, HuR (26). In addition, metallothionein 1D pseudogene (MT1DP) acts as a tumor suppressor in HCC. MT1DP negatively regulates α-fetoprotein, a liver cancer tumor marker, by inhibiting protein synthesis of forkhead box A1, an important TF in liver development and cancer progression (27). Furthermore, the lncRNA-low expression in tumor (LET), a newly identified lncRNA, is downregulated in HCC (28).
Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), is a nuclear lincRNA composed of >8,000 nucleotides that is transcribed from chromosome 11q13 (1). MALAT1 is highly expressed in pancreatic cancer and has been found to correlate with clinical stage, tumor size, lymph node metastasis and distant metastasis in pancreatic cancer patients (29). Downregulation of MALAT1 inhibits tumor cell proliferation and decreases cell migration and invasion in vitro (30). These underlying mechanisms are involved in inducing G2/M cell cycle arrest, promoting cell apoptosis, suppressing epithelial-mesenchymal transition (EMT) and reducing cancer stem-like properties (30). LncRNA highly up-regulated in liver cancer exhibits similar functions to MALAT1 (31). LncRNA BC008363 exhibits tumor suppressor activity in pancreatic cancer (32). Furthermore, LncRNA-LET has been identified as a tumor suppressor and a positive prognostic factor in gallbladder cancer (28).
Respiratory system cancers
A number of lncRNAs are significantly upregulated or downregulated in non-small cell lung cancer (NSCLC). Among these, RP11-385J1.2 and TUBA4B are the most common aberrantly expressed lncRNAs, as shown by RT-qPCR in NSCLC, which indicates that lncRNAs may exhibit an important role in the development of NSCLC (33). LncRNA PVT1 expression has been found to correlate with histological grade, lymph node metastasis and overall survival in NSCLC (34). Cheng et al (35) reported that lncRNA expression was correlated with epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) resistance in NSCLC cells. A recent study revealed that HOTAIR is involved in lung carcinogenesis induced by cigarette smoke extract (36). An additional study reported that HOTAIR promotes proliferation, invasion, metastasis and drug resistance in lung cancer cells (37). Notably, MALAT1 enhances brain metastasis by inducing EMT in NSCLC (38). Cancer-associated region lncRNA (CARLo-5) also promotes NSCLC progression, and inhibition of CARLo-5 by siRNA suppresses proliferation, migration and invasion in NSCLC cell lines (39).
Reproductive system cancers
HOTAIR is highly expressed in endometrial cancer cells and tissues. Downregulation of HOTAIR expression results in a significant inhibition of endometrial cancer cell proliferation, migration and invasion by inducing cell cycle arrest at G0/G1 phase, indicating that HOTAIR may present a key regulator of endometrial cancer (40). CASC2 functions as a tumor suppressor in endometrial cancer (23).
In addition, HOTAIR promotes the progression of human cervical cancer via the upregulation of vascular endothelial growth factor, MMP-9 and EMT-related gene expression, which are important for cell motility and metastasis (41). An additional lncRNA, GAS5, is also associated with cervical cancer; decreased GAS5 expression has been found to significantly correlate with International Federation of Gynecology and Obstetrics stage, vascular invasion, lymph node metastasis and overall survival (42).
IRAIN is an intragenic lncRNA within the insulin-like growth factor type I receptor locus with monoallelic expression from the paternal allele (43). IRAIN is aberrantly expressed in both tumors and peripheral blood leukocytes in breast cancer (BC) (43). Analysis of lncRNAs in human BC clinical subtypes revealed that HOTAIR is significantly overexpressed in the human epidermal growth factor receptor 2-enriched subgroup, while HOTAIRM1 is overexpressed in the basal-like subgroup (44). A novel lncRNA, BC040587, which is found at the 3q13.31 locus, a chromosomal region comprised of cooperatively acting tumor suppressor genes, exhibits frequent copy number alterations and is associated with BC (45). BC040587 expression is downregulated in BC samples and cell lines (45). Low expression of BC040587 is associated with a worse prognosis (45). Human ovarian cancer-specific transcript 2 promotes cell proliferation, migration and invasion in epithelial ovarian cancer by binding to miRNA let-7b, a potential tumor suppressor (46).
Urinary system cancers
MALAT1 is highly expressed in clear cell renal cell carcinoma (ccRCC) tissues and renal cancer cells when compared with adjacent non-tumor tissues, and has been found to correlate with cell proliferation, migration and invasion in ccRCC (47). According to Zhang et al (3), SPRY4-IT1 and MALAT1 exhibit identical functions, indicating that SPRY4-IT1 may present a potential prognostic biomarker and therapeutic target of ccRCC (3).
At present, prostate cancer gene antigen 3 (PCA3) is one of the most extensively studied prostate cancer-specific lncRNAs. A short tandem repeat polymorphism, TAAA, in the promoter region of the PCA3 gene has been shown to be a risk-increasing factor for prostate cancer in the Chinese population (48,49). PCA3, C20orf166-AS1 and RP11-267A15.1 are considered prostate cancer-specific lncRNAs as they exhibit significant differential expression in prostate cancer tissues (50). LncRNA ENSG00000261777 shares an intron with DEAD-box helicase DDX19 and interacts with insulin-like growth factor 2 P1, indicating its involvement in prostate cancer (5). PCAT-1-mediated proliferation is dependent on cMyc protein stabilization. The PCAT-1-cMyc relationship is mediated via the post-transcriptional activity of the MYC 3′ untranslated region. However, stabilization of cMyc may be reversed by targeting PCAT-1 with miR-3667-3p, indicating that the lncRNA PCAT-1 may promote prostate cancer proliferation via cMyc (51). Nuclear enriched abundant transcript 1 (NEAT1) is also overexpressed in prostate cancer (52).
Recently, a study demonstrated that gastric carcinoma high expressed transcript 1 (GHET1) is upregulated in bladder cancer tissue compared with adjacent normal tissues, and is correlated with tumor size, advanced tumor, lymph node status and poor survival (53). GHET1 knockdown suppresses the proliferation and invasion of bladder cancer cells and inhibiting the expression of GHET1 reverses EMT in bladder cancer cells (53).
Central nervous system cancers
HOTAIR promotes cell cycle progression in human glioma by binding its 5′ domain to the PRC2, and is closely associated with glioma stage and poor prognosis (54). Additionally, the uc.283-plus lncRNA may be important in the biology of glioma (55). Overexpression of linc-POU3F3 increases cell viability and proliferation in glioma (12). However, overexpression of CASC2 inhibits the malignancy of glioma cells via negatively regulating miR-21, subsequently affecting proliferation, migration, invasion and cellular apoptosis (23).
Other cancers
NEAT1, an lncRNA essential for the formation of nuclear body paraspeckles, is important in myeloid differentiation. It is inhibited in acute promyelocytic leukemia patients by promyelocytic leukemia/retinoic acid receptor α (56).
The expression of MALAT1 has been found to strongly correlate with disease status in multiple myeloma. The extent of changes in MALAT1 expression after treatment has prognostic relevance, with increased expression associated with poorer prognosis (57).
MALAT1 promotes the proliferation and metastasis of osteosarcoma cells by activating the phosphoinositide 3-kinase/Akt pathway (58). Notably, genetic deletion of BC040587 is associated with poor survival of osteosarcoma patients (59).
BRAF-activated lncRNA (BANCR) is overexpressed in papillary thyroid carcinoma (PTC). It may increase cell proliferation and activate autophagy in PTC (60).
Additionally, BANCR is overexpressed in melanoma. It promotes the migration of melanoma cells via the upregulation of CXCL11, an important gene that is involved in cell migration (61). Furthermore, the expression of SPRY4-IT1 is increased in melanoma cells (62). LncRNAs may also be associated with head and neck squamous cell carcinoma (63) and laryngeal squamous cell cancer (64).
Conclusion
LncRNAs exhibit an important role in a number of cancers and thus, they may be used as biomarkers for predicting recurrence and prognosis and may subsequently present potential therapeutic targets. LncRNAs exhibit significant diagnostic and therapeutic potential, however, the biological significance of lncRNAs as a group remains unclear.
In order to elucidate the mechanisms of specific lncRNAs, systematic study is required. Therefore, the development of high-throughput, high-resolution imaging and experimental techniques to advance the study of lncRNAs is required. In addition, accelerating the development of bioinformatics and establishing relevant lncRNA databases is also essential. Future studies which track lncRNA localization throughout cellular compartments and across the genome are required to determine the structure of lncRNAs and to elucidate their key structure function relationships (65). In addition, in order to investigate the post-transcriptional modification functions of lncRNAs and to perturb the cellular levels of lncRNAs in a fast and efficient manner, studies that monitor the interactions of lncRNAs with nucleic acids and proteins are required (65). In the future, we hypothesize that lncRNAs will become important components for understanding, diagnosing and treating various cancers.
Acknowledgements
This study was supported by the National Natural Science Foundation of China (grant nos. 81302093, 81272752 and 81573747) and the Hong Kong Scholars Program (grant no. XJ2015033.
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