Dioscin suppresses the viability of ovarian cancer cells by regulating the VEGFR2 and PI3K/AKT/MAPK signaling pathways
- Authors:
- Published online on: April 10, 2018 https://doi.org/10.3892/ol.2018.8454
- Pages: 9537-9542
Abstract
Introduction
Ovarian cancer is one of the three most common types of malignant tumors of the female reproductive system (1). It accounts for ~25% of female genital system neoplasms (2), with the highest mortality rate. In addition, according to official statistics, the incidence of ovarian cancer is increased in 2012 in China (1). The majority of patients with ovarian cancer are diagnosed with the disease at an advanced stage (3) and therefore, are not suitable for treatment by surgical resection due to pelvic cavity, intraperitoneal and lymphatic metastasis, and adjacent organ infiltration (4). In cases such as these, disease progression can only be controlled by relying on chemotherapeutics: however, the results are not ideal, as the survival rate is low (4). Conversely, patients diagnosed at an early stage of ovarian cancer can be treated with a cytoreductive surgery and combined chemotherapy regimen based on uranium and paclitaxel, which is associated with a relatively improved survival rate (3). Therefore, early diagnosis is a critical factor for the effective treatment of ovarian cancer.
The vascular endothelial growth factor receptor (VEGFR)2/VEGF signaling pathway serves a vital function in the angiogenesis of ovarian cancer (5) and is the primary target for antitumor angiogenesis therapy. VEGF has been demonstrated to be a pro-angiogenic factor (6). It promotes angiogenesis by inducing the growth and migration of endothelial cells, promoting the formation of vascular endothelial cells and increasing the permeability of blood vessels (7).
The downstream effects of EGFR activation primarily comprises two major signal transduction pathways: Ras/Raf/mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) and phosphoinositide 3-kinase (PI3K)/AKT, which are being increasingly studied (8). Previous studies have demonstrated that EGFR signaling primarily induces tumor cell proliferation and differentiation through the Ras/Raf/MEK/ERK signaling pathway and promotes the survival of tumor cells through the PI3K/AKT signal transduction pathway (9). Raf-1 and AKT are two important kinases in the two aforementioned pathways (10), with previous studies identifying that they are hubs for signal transduction and potentially effective targets for the treatment of ovarian cancer (10,11).
Mitogen-activated protein kinase (MAPK) is an important intracellular signal transduction system for the transfer extracellular information to the cell nucleus, thus participating in and affecting a variety of physiology and pathological processes (12). p38MAPK and ERK1/2 are essential members of these signal transduction pathways (13). It has been demonstrated that the p38MAPK and ERK1/2 signaling pathways serve essential functions in tumor incidence, drug resistance and metastasis regulation (14). The p38MAPK signal transduction pathway is associated with drug resistance in patients with ovarian cancer (14) and may also be associated with drug resistance in gastric cancer cells (13).
Diosgenin, commonly known as saponin, is a natural and synthetic steroid sapogenin (Fig. 1) (15). The relative molecular mass of diosgenin is 414.63 g/mol. Diosgenin is present at high levels in leguminosae and dioscoreaceae plants. It is an essential raw material for the synthesis of steroid hormone drugs and steroidal contraceptives (15). In the past few decades, in-depth studies on the pharmacological actions of diosgenin have been performed (16). Diosgenin has exhibited antitumor, blood lipid-regulating, anti-platelet aggregation and bilifaction promotion effects (16). It can be used as a drug for the treatment of cardiovascular disease, encephalitis, dermatosis and tumors (17). The present study aimed to investigate the effect of dioscin treatment on ovarian cancer cell growth and the mechanisms for apoptosis induction by dioscin in ovarian cancer cells.
Materials and methods
Cell line and cell culture
The ovarian cancer cell line SKOV3 was obtained from the Experiment Center of Shandong University (Shandong, China) and was cultured in minimum essential medium α (Promega Corporation, Madison, WI, USA) containing 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 100 U/ml penicillin and 100 µg/ml streptomycin (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) at 5% CO2 and 37°C.
Cell viability and apoptosis assays
SKOV3 cells were seeded into a 96-well-plate (1×103 cells/well) and treated with 0, 1.25, 2.5 and 5 µM of dioscin for 24, 48 and 72 h. Following dioscin stimulation, MTT was added to each well (0.5 mg/ml final concentration) and incubated for 4 h at 37°C. Then, old medium was removed and 150 µl dimethyl sulfoxide (99.99%) was added for the dissolution of formazan crystals. The absorbance was measured at 492 nm by an iMark™ microplate reader (Bio-Rad Laboratories, Inc., Hercules, CA, USA).
To investigate apoptosis, SKOV3 cells were seeded into a 6-well-plate (1×106 cells/well) and treated with 0, 1.25, 2.5 and 5 µM of dioscin for 48 h. Subsequently, cells were washed with PBS and stained with Annexin V-FITC/PI (5 µl/well; Nanjing KeyGen Biotech Co., Ltd., Nanjing, China) for 15 min in darkness at room temperature. The rate of apoptosis was measured using a FACSCalibur™ machine with Image Lab (version 3.0; Bio-Rad Laboratories, Inc.) and analyzed with ModFIT software (Verity Software House, Inc., Topsham, ME, USA).
Caspase-3 and caspase-9 activity
SKOV3 cells were seeded into a 6-well-plate (1×106 cells/well) and treated with 0, 1.25, 2.5 and 5 µM of dioscin for 48 h. Subsequently, cells were washed with PBS and lysed in radioimmunoprecipitation assay (RIPA) buffer (Beyotime Institute of Biotechnology, Haimen, China). Following 30 min of incubation at 37°C, the lysed cells were then centrifuged at 3,000 × g for 10 min at 4°C to pellet large cellular debris. A total of 5 µg of the pellet was incubated with caspase-3 and caspase-9 activity kits (Beyotime Institute of Biotechnology) for 1.5 h at 37°C, according to the manufacturer's protocol. The absorbance was measured using an iMark™ microplate reader at 405 nm.
Western blot analysis
SKOV3 cells were seeded into a 6-well-plate (1×106 cells/well) and treated with 0, 1.25, 2.5 and 5 µM of dioscin for 48 h. Subsequently, cells were washed with PBS and lysed in RIPA buffer (Beyotime Institute of Biotechnology). Following 30 min of incubation, the lysed cells were centrifuged at 3,000 × g for 10 min at 4°C to pellet large cellular debris. Total protein extracted was quantified with a BCA Protein Assay kit (Beyotime Institute of Biotechnology). The samples (50 µg/per lane) were heated at 100°C for 5 min, loaded onto 6–12% SDS-PAGE gels for electrophoresis and then transferred onto a polyvinylidene difluoride membrane (Bio-Rad Laboratories, Inc.). Membrane was blocked with 5% non-fat milk in TBST for 1 h at 37°C. Following incubation with the primary antibodies, including anti-Bax (cat. no. sc-6236; 1:500), anti-cleaved poly(ADP-ribose) polymerase (PARP; cat. no. sc-1562; 1:500; both Santa Cruz Biotechnology, Inc., Dallas, TX, USA), anti-VEGFR2 (cat. no. 9698; 1:2,000; Cell Signaling Technology, Inc., Danvers, MA, USA), anti-PI3K (cat. no. sc-7174; 1:500), anti-p-AKT (cat. no. sc-7985-R; 1:500), anti-phosphorylated p38MAPK (cat. no. sc-17852-R; 1:500) and anti-GAPDH (cat. no. sc-25778; 1:5,000; all Santa Cruz Biotechnology, Inc.) overnight at 4°C, the membranes were washed with TBST and incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibody (1:5,000; cat. no. BA1054; Wuhan Boster Biological Technology, Ltd. Wuhan, China) for 1 h at 37°C. Protein bands were detected by an enhanced chemiluminescence kit (Beyotime Institute of Biotechnology) and quantified by LabWorks software (version 4.0; Bio-Rad Laboratories, Inc.).
Statistical analysis
Statistical significance throughout the present study was analyzed using one-way analysis of variance and Tukey's post hoc test. Values are presented as the mean ± standard deviation from three replicates using SPSS 17.0 (SPSS, Inc., Chicago, IL, USA). P<0.05 was considered to indicate a statistically significant difference.
Results
Dioscin treatment decreased the viability of SKOV3 cells
The effects of different doses (0, 1.25, 2.5 or 5 µM for 24, 48 and 72 h) of dioscin on the viability of human ovarian cancer SKOV3 cells was investigated using an MTT assay. As presented in Fig. 2, dioscin treatment significantly decreased reduced the viability of human ovarian cancer SKOV3 cell in a dose- and time-dependent manner compared with the untreated control (P<0.01).
Dioscin treatment induced apoptosis in SKOV3 cells
To study the anticancer effects of dioscin, the rate of apoptosis in SKOV3 human ovarian cancer cells was measured following treatment with 0, 1.25, 2.5 and 5 µM of dioscin for 48 h with flow cytometry. Compared with the 0 µM control group, dioscin significantly increased cellular apoptosis in a dose-dependent manner (P<0.01; Fig. 3).
Dioscin treatment promoted caspase-3 and caspase-9 activity in SKOV3 cells. In order to investigate the endogenous regulatory function of caspase-3 and caspase-9 in human ovarian cancer, the present study measured caspase-3 and caspase-9 activity in SKOV3 cells. As presented in Fig. 4, caspase-3 and caspase-9 activity in the SKOV3 cells was significantly increased following treatment with 1.25, 2.5 or 5 µM dioscin, compared with the untreated control (P<0.01).
Dioscin treatment increased Bax and cleaved PARP protein expression in SKOV3 cells
To explore the anticancer effects of dioscin in human ovarian cancer, Bax protein expression level was determined using a western blot analysis. Western blot analysis demonstrated that the effects of dioscin (1.25, 2.5 or 5 µM) significantly increased Bax and cleaved PARP protein expression levels in SKOV3 cells, compared with the untreated control group (P<0.01; Fig. 5).
Dioscin treatment suppressed PI3K/AKT, VEGFR2 protein expression and induced p-p38 protein expression in SKOV3 cells
To investigate the role of PI3K/AKT, VEGFR2 and p-p38 in the anticancer effects of dioscin treatment of SKOV3 cells, the present study performed western blot analysis. As presented in Fig. 6, treatment with 2.5 µM or 5 µM dioscin significantly suppressed PI3K and phosphorylated (p)-AKT, VEGFR2 protein expression, and induced p-p38 protein expression in SKOV3 cell, compared with the control group (P<0.01).
Discussion
Primary epithelial ovarian cancer is the most fatal type of malignant gynecological tumor, with a global fatality rate of ~6.2% (4). In 2012, there were ~22,000 new cases of ovarian cancer and ~15,500 associated mortalities in the United States (4). This high mortality rate is due to chemotherapy resistance and the lack of effective screening methods (18). Although >70% of patients with ovarian cancer can be treated effectively with surgery, chemotherapy and second-line chemotherapy, novel and more effective pharmacological intervention is required due to the low overall cure rates and serious adverse effects associated with chemotherapy (19). The results of the present study indicated that dioscin significantly promoted caspase-3 and caspase-9 activity, increased Bax protein expression and enhanced cleaved PARP protein expression in SKOV3 cells.
VEGF mediates cell synthesis, stimulates cellular proliferation and migration, and enhances the permeability of blood vessels through the effects of VEGFR on vascular endothelial cells (20). Local increases in VEGF concentration may promote the upregulation of the expression of vascular endothelial cell VEGFR, which is an important mechanism to allow VEGF in tumor tissue to promote angiogenesis (20). VEGFR2 is highly expressed on the endothelial cell surface of new blood vessels in a tumor, and promotes the growth of the new endothelial cells required by tumor angiogenesis (21). The expression percentage and expression intensity of VEGFR2 is considered to be an important indicator for the evaluation of tumor prognosis (22). Consequently, VEGFR2 is an essential factor for tumor anti-angiogenesis therapy (22). The results of the present study demonstrated that treatment with dioscin significantly suppressed VEGFR2 protein expression in SKOV3 human ovarian cancer cells compared with the untreated control group. A previous report by Tong et al (15) suggested that dioscin inhibits colon tumor growth through regulating VEGFR2 signaling pathways.
EGFR and its ligands extensively exist on epithelium, stroma, original nerve cells and various microstructures (23). EGFR is activated by dimerization and phosphorylation following the binding of exogenous substrates; in turn, it activates the downstream Ras/Raf/MAPK and Ras/PI3K/AKT/mTOR signaling pathways, thus controlling a variety of the biological responses of mature tissues (24). In addition, EGFR and the associated pathways are essential in embryonic development and tissue differentiation (24). EGFR serves an important function in regulating the growth and differentiation of normal cells as well as promoting the growth of tumor and mesenchymal cells, tumor cell adhesion and apoptosis resistance, and tumor metastasis and angiogenesis (25). In addition, previous studies have demonstrated that the overexpression of EGFR is associated with drug resistance in ovarian cancer (25). The results of the present study revealed that dioscin significantly suppressed PI3K and p-AKT protein expression in SKOV3 cells, compared with the untreated control group. Hsieh et al (26) demonstrated that dioscin induced human lung cancer cell apoptosis through modulation of the PI3K/Akt signaling pathway.
The effect of number of growth factors and receptors associated with tumor growth and metastasis processes is mediated via the p38MAPK signaling pathway (27). Tumors are generated as a response to the dysregulation of intracellular signaling (27); the p38MAPK pathway is an essential signaling pathway for the transduction of signals to the nucleus (28), with functions in cell growth, proliferation, cell death, cell cycle, inflammation and stress reactions (29). In addition, the p38MAPK signal transduction pathway may inhibit tumor formation (30) and regulate the synthesis of interleukin (IL)-1β precursor, thus promoting IL-1β synthesis (31). IL-1β coordinates immunological function, activating lymphocytes to promote proliferation, promoting the generation of antibodies and activated cytokines, enhancing the cytotoxicity of natural killer cells and creating a positive feedback effect that effectively kills tumor cells (31). The results of the present study indicated that treatment with dioscin significantly induced the p-p38 MAPK protein expression of SKOV3 human ovarian cancer cells compared with the untreated control group. In accord with this result, Wang et al (17) demonstrated that dioscin induced the apoptosis of HL-60 acute myeloid leukemia cells through the activation of p38MAPK and Jun N-terminal kinase.
In conclusion, the results of the present study demonstrated for the first time, to the best of our knowledge, that dioscin suppresses cellular viability and induces apoptosis in ovarian cancer cells through the suppression of the VEGFR2 and PI3K/AKT/p38 MAPK signaling pathways. Thus, dioscin may have potential as an anticancer drug, and the VEGFR2 and PI3K/AKT/p38 MAPK signaling pathway may represent a potential target for anticancer agents.
Acknowledgements
Not applicable.
Funding
No funding was received.
Availability of data and materials
The analyzed data sets generated during the study are available from the corresponding author on reasonable request.
Authors' contributions
XD designed the study. XG performed the experiments. XG and XD analyzed the data. XD wrote the manuscript.
Ethics approval and consent to participate
Not applicable.
Consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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