Clinical value of TV-CDS combined with serum tumor markers in diagnosis of ovarian cancer
- Authors:
- Published online on: June 5, 2020 https://doi.org/10.3892/ol.2020.11705
- Pages: 2028-2034
Abstract
Introduction
Ovarian cancer (OC) is one of the common malignant tumors in the female reproductive system. Its incidence rate ranks third below cervical cancer and carcinoma of corpus uteri, and its mortality rate ranks first among all kinds of gynecologic tumors, posing a serious threat to women's health (1). As the ovary is located in the deep part of the pelvic cavity, early symptoms are not typical. Most patients have developed middle and late stage OC when they seek medical treatment, missing the best treatment opportunity (2).
At present, the conventional methods for clinical screening of OC are trans-abdominal sonography and the determination of serum tumor marker carbohydrate antigen 125 (CA125). However, trans-abdominal sonography has many influencing factors, and the ratio of false positive and false negative in diagnosis of OC are high, resulting in poor stability and accuracy. The detection of serum CA125 alone has poor sensitivity and accuracy, so the early diagnosis of OC is not ideal (3). In recent years, transvaginal color doppler ultrasound (TV-CDS) technology is an emerging examination method, which can obtain clearer mass morphology, internal echo and blood flow images, providing reference for the diagnosis of OC by measuring blood flow parameters (4). At the same time, with the rapid development of molecular biology, an increasing number of tumor markers are closely related to OC, such as CA125, vascular endothelial growth factor (VEGF), and osteopontin (OPN).
In this study, TV-CDS combined with serum CA125, VEGF and OPN were selected to diagnose OC, so as to provide basis for early diagnosis, monitor of disease progression and evaluation of prognosis of OC.
Patients and methods
Clinical materials
From July 2017 to December 2018, 106 patients with ovarian cancer (MTG) and 50 patients with benign ovarian diseases (BCG) who received initial treatment in The Third People's Hospital of Qingdao (Qingdao, China) were selected as the study subjects. Patients in MTG were aged 35-83 years, with an average age of 56.72±9.34. Clinical staging referred to the Federation International of Gynecology and Obstetrics (FIGO) staging standard in 2009: A total of 11 cases in stage I, 39 cases in stage II, 44 cases in stage III and 12 cases in stage IV. The histopathological types were 69 cases of ovarian serous adenocarcinoma, 19 cases of ovarian mucinous adenocarcinoma, 11 cases of endometrial carcinoma and 7 cases of clear cell carcinoma. According to WHO classification criteria, there were 23 cases of well differentiated, 35 cases of middle differentiation and 48 cases of undifferentiated. Patients in BCG were 34-86 years of age, with an average age of 57.26±10.98, including 29 cases of serous cystadenoma, 10 cases of mucinous cystadenoma, 6 cases of ovarian teratoma and 5 cases of endometrial cyst.
Inclusion criteria were: i) Patients treated for the first time without radiotherapy and chemotherapy or endocrine therapy; ii) patients who underwent TV-CDS, serum CA125, VEGF and OPN; iii) patients who underwent surgery or laparoscopic treatment in the hospital and were diagnosed by pathology and iv) patients with complete follow-up data.
Exclusion criteria were: i) Incomplete medical history data; ii) other important organ dysfunction and iii) combined with other tumors.
The study was approved by the Ethics Committee of The Third People's Hospital of Qingdao. All patients who participated in this research had complete clinical data. Patients provided a signed informed consent and volunteered to participate in the study. Age and other baselines of the two groups were not statistically significant, and thus, the groups were comparable.
TV-CDS examination
PHILIPS iU22 (Koninklijke Philips N.V.) was used for color doppler ultrasound diagnosis, with a transducer frequency of 5-7 MHz. The patient was instructed to empty the bladder before examination. During examination, the patient lay on the back in a lithotomy position. The transvaginal ultrasound probe was covered with a disposable latex cot or condom. The cot was coated with disinfectant couplant. The probe was placed in the deepest part of the vagina. The size, morphology, boundary, internal echo, calcification, side-acoustic images and other imaging conditions of the lesion were explored (5). Then the blood flow were observed using color doppler flow imaging (CDFI) technology, including flow classification, resistance index (RI) and pulsability index (PI). Flow classification (6): Grade 0: there was no blood flow signal; Grade I: There was punctiform or short line blood flow around the tumor but there was no blood flow signal inside the tumor. Class II: There was punctiform or short line blood flow inside the tumor, with regular blood vessel course; Class III: the blood flow inside the tumor was dendritic and reticular, with abundant short and long blood flow, tortuosity and disordered blood vessels. RI and PI were directly read out and counted by ultrasonic instruments. The ultrasonography, recording and image diagnosis of all patients were determined by two deputy directors of the ultrasonography department.
Examination of serum tumor markers
A total of 4 ml fasting elbow venous blood was extracted from OC patients before treatment and at 3 months after treatment, and from the subjects in the BCG group at 6.00-9.00 in the morning. The serum was self-coagulated at room temperature, and then centrifuged using DT5-4 automatic decap centrifuge (Beijing Era Beili Centrifuge Co., Ltd.) at 2,264 × g, at room temperature for 30 min. CA125 was detected by electrochemiluminescence using Roche ELecsys-2010 and Roche original reagents. VEGF and OPN were detected by enzyme-linked immunosorbent assay (ELISA). Reagents were provided by R&D Systems, Inc. Tecan Infinite M1000 PRO multi-function enzyme-labeling instrument (Tecan Group, Ltd.) was used according to the manufacturer's instructions. The reference values were CAl25 ≤35.00 U/ml, VEGF ≤148.60 pg/ml and OPN ≤49.90 ng/ml.
Result estimation
One or more positives in the combined examination were considered positive. All negatives were considered as negative.
Evaluation index
The standard method of diagnostic test evaluation: Subjects were divided into patients and non-patients by gold standard diagnosis, a certain test method was used to measure the positive and negative results, and then various statistical analysis were carried out. Based on this, the evaluation indexes commonly used in diagnostic tests can be calculated. The results of the test diagnosis were divided into (a) true positive, (b) false positive, (c) false negative and (d) true negative. Calculation formula: Sensitivity = a/(a+c); Specificity = d/(d+b); Accuracy = (a+d)/(a+b+c+d); Positive predictive value = a/(a+b); Negative predictive value = d/(d+c).
Statistical analysis
Excel 2007 was used to establish a database. SPSS17.0 statistical software, t-test and χ2 test were applied. The concentration level of the measured data was expressed as mean ± standard deviation (SD). The comparison of the mean number between groups was conducted by independent sample t-test. The comparison of the counting data rate was conducted by χ2 test, with statistical significance (P<0.05).
Results
Sonographic comparison of OC and benign ovarian diseases by TV-CDS
According to the histopathological ‘gold standard’ and the diagnostic value of TV-CDS for benign and malignant ovarian tumors, the sensitivity of TV-CDS in the diagnosis of OC was 75.47% (80/106) and the specificity was 90.00% (45/50). TV-CDS showed that the incidence of lesions in conventional two-dimensional ultrasound including irregular morphology, unclear boundary, uneven echo, microcalcification and side-acoustic images in OCG was significantly higher than that in BCG (P<0.01), as shown in Table I. Regarding blood flow grading, most patients in the MTG were in grade II and III, while most patients in the BCG were in grade 0. Compared with RI (0.73±0.09) and PI (1.25±0.15) in BCG, RI (0.38±0.04) and PI (0.63±0.09) in the OCG were significantly decreased (t= −3.893, −3.883, P<0.01) (Table II). The ultrasonic acoustic and blood flow display images of OC are shown in Fig. 1.
 | Table I.Comparison of conventional ultrasonographic features of lesion location of patients in the two groups [n (%)]. |
Comparison of serum CA125, VEGF, OPN levels of patients between OCG and the control group
The levels of serum CA125, VEGF and OPN of patients in OCG were significantly higher than those in BCG, with statistically significant difference (P<0.01) (Table III).
| Table III.Comparison of serum CA125, VEGF and OPN levels of patients between OCG and the control group (mean ± SD). |
Comparison of expression levels of serum CA125, VEGF and OPN in different clinicopathological factors of OC
The expression levels of serum CA125, VEGF and OPN in OC patients with clinical high stage (stage III and IV), poorly differentiated, ascites, recurrence and metastasis were significantly higher than those in patients with clinical low stage (stage I and II), well differentiated, no ascites and no recurrence and metastasis (P<0.05). With the disappearance of the tumor or the decrease of tumor load, the serum marker levels after treatment were significantly lower than those before treatment (P<0.05) (Table IV).
| Table IV.Comparison of expression levels of serum CA125, VEGF and OPN in different clinicopathological factors of ovarian cancer (mean ± SD). |
Comparison of diagnostic value of transvaginal ultrasound, CA125, VEGF, OPN single and combined examination in OC
The sensitivity, accuracy and negative predictive value of the combination of transvaginal ultrasound, CA125, VEGF and OPN in the diagnosis of OC were significantly improved compared with those of individual and partial combination tests. The differences were statistically significant (P<0.05) (Table V).
| Table V.Diagnostic value of transvaginal ultrasound, CA125, VEGF, OPN single and combined examination in ovarian cancer [% (ratio)]. |
Discussion
Compared with the traditional transvaginal color Doppler imaging (TV-CDS), the transvaginal ultrasound is closer to the ovary. The frequency of the probe is higher without interference from abdominal wall fat and flatulence, which significantly improves image quality and resolution and makes it more objective and accurate to judge whether the ovarian tumors are benign or malignant. Schelling et al (4) stated that two important factors for the diagnosis of OC by ultrasound are the determination of the solid component of the lesion and the presence of blood flow signals in the solid component. The benign and malignant ovarian masses were judged by color Doppler ultrasound two-dimensional image and blood flow imaging (7). The results of this study showed that the incidence of irregular lesion shape, unclear boundary, uneven internal echo, microcalcification and lateral sound shadow in the OC group was significantly higher than that in the benign lesion group. Due to the lack of muscle layer in the neovasal wall of ovarian malignant tumor, the neovasal wall is highly permeable and the vascular wall is thin, presenting a low-resistance spectrum, and the RI and PI of OC are significantly lower than those of benign ovarian tumors (8). Kurjak et al (9) proposed that the sensitivity of RI≤0.4 in the diagnosis of ovarian malignancy was 86.4%. The results of this study showed that the sensitivity of TV-CD in the diagnosis of OC was 75.47%, slightly lower than those in previous reports, which may be related to the low threshold of RI (≤0.35). A study on early detection of OC screening was carried out in the United States, 4,526 women at high risk of developing OC were screened by transvaginal ultrasound. The value of single detection method of screening was limited, in particular, early ovarian patients with low blood flow was easily misdiagnosed (10). Therefore, combined detection with other markers is needed.
The detection of serum tumor markers is a common detection method in the diagnosis of malignant tumors, because it is simple and inexpensive, and the samples are easy to collect. CA125 is still the most commonly used biomarker for the diagnosis and efficacy monitoring of OC, providing valuable information for the determination of the benign and malignant ovarian tumors (11). It has been reported that CA125 is elevated in the serum of most ovarian epithelial carcinoma, and continuous elevation of CA125 level indicates a relatively poor prognosis (12). It was better in the prognosis of patients where CA125 level declined to normal after 3 months of treatment, compared with those who did not return to normal. The sensitivity of CA125 in the diagnosis of OC in this study was 60.38%, which was relatively lower than the 73.60% reported by Schummer et al (13), which may be related to the difference in selected cases. The expression level of CA125 in mucous ovarian epithelial carcinoma is low, which is prone to false negatives. In female inflammatory diseases, ovarian chocolate cyst, follicular cyst, pregnancy and menstruation, CA125 is increased to different degrees (14), which affects the accurate diagnosis of OC and results in false positive, indicating that the value of CA125 alone is limited. VEGF is a cytokine that regulates angiogenesis and acts specifically on vascular endothelial cells. The role of VEGF in tumors is not limited to the formation of blood vessels and the regulation of vascular permeability (15). Previous research has shown that the presence of VEGF regulatory signal is found in tumor cells, which not only acts on the formation of tumor cells, but also plays a certain role in the formation of tumor stem cells and tumor metastasis (16). VEGF plays an important role in the formation of lymphatic vessels (17). As an important factor of tumor angiogenesis, VEGF plays a very important role in the occurrence, progression and invasion of tumors (16). Serum expression level is significantly correlated with the clinical stage, invasion, recurrence and metastasis of tumors (18). The results of this study showed that the sensitivity of VEGF in the diagnosis of OC was 58.49%. Li et al (19) reported that the sensitivity of VEGF in the diagnosis of OC was 77.00% in the combined detection of VEGF and CA125, indicating that the value of VEGF alone was limited.
OPN is a glycosylated phosphorylated protein that is associated with the development, invasion and metastasis of tumors. Its expression is significantly up-regulated in a variety of malignant tumor tissues (20). In terms of the mechanism of tumor angiogenesis, VEGF can play a synergistic role with OPN to promote the generation of granulation tissue and induce angiogenesis. The establishment of new blood vessels can promote cell migration, adhesion and prevent cell apoptosis, which is conducive to tumor cell generation (21). The increase of OPN level in peripheral blood of advanced OC is more obvious, and the serum expression level is positively correlated with clinical stage (22). As for the mechanism of tumor invasion, metastasis and relapse, cell adhesion - GRGDS sequence in OPN may interact with integrin and cancer cell surface adhesion molecule CD44 to inhibit endothelial cell apoptosis, and cause a variety of protein dissolving enzyme synthesis and secretion, so as to dissolve the extracellular matrix barrier to tumorigenesis of invasion and metastasis (23). Relevant research has shown that OPN is closely correlated with the development of OC (21). Kim et al (24) studied 107 plasma samples by cDNA array and found that there was a significantly high level of OPN expression in invasive OC and ovarian junction tumors. It has been pointed out that OPN positive expression could be detected in OC patients with negative CA125, and the detection of OPN was complementary (25). The results of this study showed that the sensitivity of OPN in the diagnosis of OC was 55.66%, indicating that the value of detection alone was limited. Combined detection with other markers can enhance the value.
The results of this study showed that transvaginal ultrasound and serum CA125, TSGF and VEGF have their respective advantages and disadvantages in the diagnosis of OC, and the sensitivity and accuracy are not high, which is far from the clinical requirements. Because the combined parallel method was used, that is, if any of the indicators is positive, it is determined to be positive. Although the specificity decreased (84.00%), the sensitivity, accuracy and negative predictive value (97.17, 93.59, and 93.48%, respectively) were significantly higher than those of individual examinations, thereby missed diagnosis was reduced.
In conclusion, TV-CDS combined with serum CA125, TSGF, and VEGF can be used as an effective method for the diagnosis of OC. The selected sample size for this study was limited. Therefore, research on larger sample size and prognostic follow-up will be necessary.
Acknowledgements
Not applicable.
Funding
No funding was received.
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Authors' contributions
CY was involved in the conception of the study and wrote the manuscript. TD and YL collected and analyzed the general data of the patients. RL assisted with the statistical analysis. All authors read and approved the final manuscript.
Ethics approval and consent to participate
The study was approved by the Ethics Committee of The Third People's Hospital of Qingdao (Qingdao, China). All patients who participated in this research had complete clinical data. Signed informed consents were obtained from the patients and/or guardians.
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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