Mutational analysis of 9 different tumour-associated genes in human malignant mesothelioma cell lines
- Authors:
- Published online on: September 1, 2005 https://doi.org/10.3892/or.14.3.743
- Pages: 743-750
Metrics: Total
Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Abstract
Seven tumour suppressor genes (Chk1, Chk2, Apaf1, Rb1, p53, p16INK4a and p14ARF) and two oncogenes (N-ras and BRAF) were screened in nine human malignant melanoma (HMM) cell lines for point mutations or small deletions/insertions by DGGE, TGGE and SCCP analysis. For the first time in human mesothelioma, Chk1 gene mutations were detected in two of the nine investigated HMM cell lines. P53 gene mutations were found in three cell lines and p16INK4a mutations in 5. Mutation of the Chk1 gene implies a novel disruption mechanism of the p53 pathway in HMM, without affecting p53 itself. According to our knowledge, this is the first mutation screening of Chk1, Chk2, Apaf1 and Rb1 in human malignant mesothelioma.
