Reduced radiosensitivity and increased CD40 expression in cyclophosphamide-resistant subclones established from human cervical squamous cell carcinoma cells

  • Authors:
    • Tetsuji Tanaka
    • Tao Bai
    • Kazunori Yukawa
    • Tomoko Utsunomiya
    • Naohiko Umesaki
  • View Affiliations

  • Published online on: October 1, 2005     https://doi.org/10.3892/or.14.4.941
  • Pages: 941-948
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Abstract

To investigate the interaction between anticancer drug resistance and radioresistance in cervical cancer cells, 3 single cell-derived cyclophosphamide-resistant subclones were established from the drug- and radiosensitive human cervical squamous cell carcinoma cell line ME180 by chronic exposure cultures with 4-hydroxy-cyclophosphamide followed by limiting dilution. The established cyclophosphamide-resistant subclones were also radio- and multidrug-resistant to 7 other anticancer drugs. Flow cytometric analysis revealed significantly increased levels of CD40 expression on the 3 resistant subclones, whereas no CD40 expression was found on the parent ME180 cells. However, there were no changes in the expression levels of CD29, CD49a-CD49f or CD59 between the parent cells and resistant subclones. A recombinant human soluble CD40 ligand had no effect on the proliferation of the resistant subclones. Irradiation had no effect on the 4-hydroxy-cyclophosphamide sensitivity of the parent cells. These results indicate that the established cyclophosphamide-resistant subclones have impaired cell death signals, which are common to both drug- and radiation-induced apoptosis, and cyclophosphamide may not be an adequate drug for use in concurrent chemoradiotherapy. Furthermore, CD40 activation signals may be associated with the multidrug- and radioresistance in these cyclophosphamide-resistant subclones.

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October 2005
Volume 14 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Tanaka T, Bai T, Yukawa K, Utsunomiya T and Umesaki N: Reduced radiosensitivity and increased CD40 expression in cyclophosphamide-resistant subclones established from human cervical squamous cell carcinoma cells. Oncol Rep 14: 941-948, 2005.
APA
Tanaka, T., Bai, T., Yukawa, K., Utsunomiya, T., & Umesaki, N. (2005). Reduced radiosensitivity and increased CD40 expression in cyclophosphamide-resistant subclones established from human cervical squamous cell carcinoma cells. Oncology Reports, 14, 941-948. https://doi.org/10.3892/or.14.4.941
MLA
Tanaka, T., Bai, T., Yukawa, K., Utsunomiya, T., Umesaki, N."Reduced radiosensitivity and increased CD40 expression in cyclophosphamide-resistant subclones established from human cervical squamous cell carcinoma cells". Oncology Reports 14.4 (2005): 941-948.
Chicago
Tanaka, T., Bai, T., Yukawa, K., Utsunomiya, T., Umesaki, N."Reduced radiosensitivity and increased CD40 expression in cyclophosphamide-resistant subclones established from human cervical squamous cell carcinoma cells". Oncology Reports 14, no. 4 (2005): 941-948. https://doi.org/10.3892/or.14.4.941