Gene expression profiling of oral submucous fibrosis using oligonucleotide microarray

  • Authors:
    • Yanjia Hu
    • Xinchun Jian
    • Jieying Peng
    • Xiaowen Jiang
    • Ning Li
    • Shanghui Zhou
  • View Affiliations

  • Published online on: August 1, 2008     https://doi.org/10.3892/or_00000005
  • Pages: 287-294
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Although oral submucous fibrosis (OSF) is the most common precancerous lesion of the oral cavity in Southeast Asia where the habit of betel quid (BQ) chewing is popular, its molecular biological properties are largely unknown. The aim of this study was to identify the genes responsible for its pathogenesis and malignant transformation using oligonucleotide microarray. The expression profiles of 14,500 genes in human oral submucous fibrosis and normal control were analyzed using Affymetrix U133A 2.0 GeneChip arrays. The results revealed that 716 genes were upregulated and 149 genes were downregulated in OSF. Hierarchical clustering revealed that the gene expression profiles of normal and OSF were clearly distinct by these different expression genes. Gene Ontology (GO) and relevant bioinformatics tools identified a list of significant differentially expressed genes involved in immune response, inflammatory response and epithelial-mesenchymal transition (EMT) induced by TGF-β signaling pathway. Five EMT-related genes including SFRP4, THBS1, MMP2, ZO-1, and CK18 were validated with RT-PCR. Our data suggested that gene abnormalities in immune response, inflammatory response and EMT induced by TGF-β might play an important role in the pathogenesis and malignant transformation of OSF.

Related Articles

Journal Cover

August 2008
Volume 20 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Hu Y, Jian X, Peng J, Jiang X, Li N and Zhou S: Gene expression profiling of oral submucous fibrosis using oligonucleotide microarray. Oncol Rep 20: 287-294, 2008.
APA
Hu, Y., Jian, X., Peng, J., Jiang, X., Li, N., & Zhou, S. (2008). Gene expression profiling of oral submucous fibrosis using oligonucleotide microarray. Oncology Reports, 20, 287-294. https://doi.org/10.3892/or_00000005
MLA
Hu, Y., Jian, X., Peng, J., Jiang, X., Li, N., Zhou, S."Gene expression profiling of oral submucous fibrosis using oligonucleotide microarray". Oncology Reports 20.2 (2008): 287-294.
Chicago
Hu, Y., Jian, X., Peng, J., Jiang, X., Li, N., Zhou, S."Gene expression profiling of oral submucous fibrosis using oligonucleotide microarray". Oncology Reports 20, no. 2 (2008): 287-294. https://doi.org/10.3892/or_00000005