Effects of two organotin(IV)(sulfonatophenyl)porphinates on MAPKs and on the growth of A375 human melanoma cells

  • Authors:
    • Maria Assunta Costa
    • Letizia Gulino
    • Lorenzo Pellerito
    • Tiziana Fiore
    • Claudia Pellerito
    • Giovanna Barbieri
  • View Affiliations

  • Published online on: March 1, 2009     https://doi.org/10.3892/or_00000261
  • Pages: 593-599
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Abstract

Previously we showed apoptotic induction in A375 human melanoma cells using two complexes of the meso-tetra(4-sulfonatophenyl)porphinate (TPPS), (Bu2Sn)2TPPS and (Bu3Sn)4TPPS. To understand how these compounds activate apoptosis in melanoma cells we studied MAPKs and the (Bu2Sn)2TPPS and (Bu3Sn)4TPPS cellular uptake. Western blotting experiments showed activated protein kinases ERK 1/2, JNK and p38 in 10 µM (Bu2Sn)2TPPS- and 1 µM (Bu3Sn)4TPPS-treated melanoma cells, which suggests that the three MAP kinases are involved in the apoptotic death of A375-treated cells. By taking advantage of the porphyrin fluorescence, we found a fast concentration of (Bu2Sn)2TPPS and (Bu3Sn)4TPPS in the nucleus and in the nucleoli compared to TPPS. A significantly reduced growth of A375 human melanoma cells was also observed after only 48 h treatment by using 500 nM of (Bu2Sn)2TPPS or 80 nM of (Bu3Sn)4TPPS. A strong slowdown of cell growth and loss of cell-cell interactions were visible by in vitro wound repair assay.

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March 2009
Volume 21 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Costa MA, Gulino L, Pellerito L, Fiore T, Pellerito C and Barbieri G: Effects of two organotin(IV)(sulfonatophenyl)porphinates on MAPKs and on the growth of A375 human melanoma cells. Oncol Rep 21: 593-599, 2009.
APA
Costa, M.A., Gulino, L., Pellerito, L., Fiore, T., Pellerito, C., & Barbieri, G. (2009). Effects of two organotin(IV)(sulfonatophenyl)porphinates on MAPKs and on the growth of A375 human melanoma cells. Oncology Reports, 21, 593-599. https://doi.org/10.3892/or_00000261
MLA
Costa, M. A., Gulino, L., Pellerito, L., Fiore, T., Pellerito, C., Barbieri, G."Effects of two organotin(IV)(sulfonatophenyl)porphinates on MAPKs and on the growth of A375 human melanoma cells". Oncology Reports 21.3 (2009): 593-599.
Chicago
Costa, M. A., Gulino, L., Pellerito, L., Fiore, T., Pellerito, C., Barbieri, G."Effects of two organotin(IV)(sulfonatophenyl)porphinates on MAPKs and on the growth of A375 human melanoma cells". Oncology Reports 21, no. 3 (2009): 593-599. https://doi.org/10.3892/or_00000261