Human umbilical cord mesenchymal stem cells increase interleukin-9 production of CD4+ T cells
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- Published online on: August 17, 2017 https://doi.org/10.3892/etm.2017.4952
- Pages: 3541-3548
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Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
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Abstract
Mesenchymal stem cells (MSC) are able to differentiate into cells of multiple lineage, and additionally act to modulate the immune response. Interleukin (IL)‑9 is primarily produced by cluster of differentiation (CD)4+ T cells to regulate the immune response. The present study aimed to investigate the effect of human umbilical cord derived‑MSC (UC‑MSC) on IL‑9 production of human CD4+ T cells. It was demonstrated that the addition of UC‑MSC to the culture of CD4+ T cells significantly enhanced IL‑9 production by CD4+ T cells. Transwell experiments suggested that UC‑MSC promotion of IL‑9 production by CD4+ T cells was dependent on cell‑cell contact. Upregulated expression of CD106 was observed in UC‑MSC co‑cultured with CD4+ T cells, and the addition of a blocking antibody of CD106 significantly impaired the ability of UC‑MSC to promote IL‑9 production by CD4+ T cells. Therefore, the results of the present study demonstrated that UC‑MSC promoted the generation of IL‑9 producing cells, which may be mediated, in part by CD106. The findings may act to expand understanding and knowledge of the immune modulatory role of UC‑MSC.
