Role and effective therapeutic target of gut microbiota in NAFLD/NASH (Review)
- Authors:
- Published online on: July 17, 2019 https://doi.org/10.3892/etm.2019.7781
- Pages: 1935-1944
Abstract
Introduction
Non-alcoholic fatty liver disease (NAFLD) has become the most prevalent chronic liver disease in the world; an estimated 6–35% of the worldwide are affected and the prevalence of NAFLD has been increasing in recent years due to the improvement of living standards (1,2). It is estimated that NAFLD affects one-third of all adults in the US, and attention to NAFLD in Asian populations has also been paid over the past decades (3–6). NAFLD is characterized by the accumulation of hepatic triglyceride (TG) that exceeds 5% of the total liver weight (7–9). Hepatic steatosis is regarded as the benign beginning of NAFLD, as no severe liver injury is observed during this stage, but certain patients with hepatic steatosis progress to non-alcoholic steatohepatitis (NASH), NASH-associated cirrhosis and hepatocellular carcinoma (HCC) (6,10). Biopsy remains the golden standard for the diagnosis of NAFLD, and in addition, various non-invasive diagnostic methods, including hepatic ultrasonography, computed tomography, and magnetic resonance imaging have been used for detecting hepatic fatty infiltration. Furthermore, magnetic resonance elastography and transient elastography have been frequently used for diagnosing NAFLD with advanced fibrosis (11–13).
NAFLD is a complex disease that may be attributed to a combination of factors, including genetics, diet and gut microbiota (14,15); however, the detailed pathogenesis of NAFLD has remained to be fully elucidated. Common risk factors of NAFLD include aging, lifestyle, central obesity-associated insulin resistance (IR) and the development of metabolic syndrome (16–18). In 1998, Day and James (19) published a theory on the pathogenesis of NAFLD called the ‘two hits’ hypothesis, according to which lipid deposition in the liver was the first hit, while the subsequent second hit includes oxidative stress, lipid peroxidation, lipotoxicity and mitochondrial dysfunction. It was the second hit that contributed to the development of NASH. The more recently postulated ‘multiple parallel’ hits hypothesis suggested that inflammatory mediators from various tissues, particularly the adipose and gut tissues, participate in the activation of inflammation, progression of fibrosis and the tumorigenesis (20,21). There is a clear understanding that carriers of certain common genetic variants, including patatin-like phospholipase domain-containing 3, transmembrane 6 superfamily member 2, membrane-bound O-acyltransferase domain-containing 7 and microsomal TG transfer protein, increase the risk of developing severe forms of NAFLD (22–25).
In recent years, increasing attention has been paid to the role of the gut microbiota in the pathogenesis of NAFLD. Accumulated data proved the significant effects of the gut microbiota on liver function in vivo, which may contribute to numerous physiological processes, including energy metabolism, obesity, type 2 diabetes mellitus (T2DM) and NAFLD (26–28). In the present review, recent evidence for the key roles of the gut microbiota in the development and progression of NAFLD was summarized and the underlying mechanisms were discussed. In addition, the therapeutic potential of anti-diabetic drugs on NAFLD via targeting the gut microbiota was reviewed.
Gut microbiota
An estimated 10–100 trillion microbes exist in the human body and most of them are settled in the gut, particularly in the large intestine, reaching a maximum number of up to ~1014 and a weight of ~1.5 kg (29,30). In spite of the wide microbial diversity, only 4 bacterial phyla dominate the gut: Firmicutes, Bacteroidetes, Actinobacteria and Proteobacteria, with Firmicutes and Bacteroidetes accounting for ~90% of all gut microbes (31,32). The composition of gut microbiota is variational among different individuals. The ratio of Firmicutes vs. Bacteroidetes has been frequently reported to be associated with the susceptibility to diseases (33,34). It is difficult for traditional microbial technology to obtain a detailed list of gut microbiota, but with the adoption of 16S ribosomal RNA gene sequencing, an unprecedented view of the diversity of gut microbiota has been gained (34).
Normal gut microbiota may produce numerous substances that provide a benefit for the health of the host via regulation of immunity, supplementation of nutrition and homeostasis, and the gut microbiota has co-evolved with immune system in the regulation of several metabolic pathways (35–37). Accumulated evidence has indicated that the gut microbiota interacts with the liver via the so-called the ‘liver-gut axis’, and certain specific metabolites are involved, including bile acids (BAs), lipopolysaccharides (LPS) and short-chain fatty acids (SCFAs) (38). Lipid and carbohydrate metabolism involving several types of bacteria are associated with obesity-associated energy metabolism. Therefore, disorders linked to energy metabolism, including hyperlipidemia, atherosclerosis, diabetes and inflammation, may be regulated by the gut microbiota and their metabolites (35,39,40).
Gut dysbiosis and NAFLD
Although an enormous number of microbiota exist in the gut, the proportion and quantity of each species is relatively stable under normal conditions. Changes in the internal and external environment of the host, which may include diet, alcohol intake, antibiotics and genetic factors, may affect the stability of the gut microbiota, resulting in gut microbiota dysbiosis (41). Emerging evidence points out that gut microbiota dysbiosis has a significant role in the pathogenesis of human liver diseases, particularly in NAFLD and associated metabolic disorders (15).
Animal experiments provide strong evidence to support the role of gut microbiota dysbiosis in the development of obesity and NAFLD. In an earlier study, it was indicated that the microbiome in obese mice was associated with increased gain of intestinal energy from the diet. When these mice were co-housed with lean adult germ-free mice for 14 days, this feature was also observed in the previously germ-free mice (42). Membrez et al (43) indicated that the gut microbiota alters the expression of hepatic and intestinal genes involved in the inflammatory, hormonal and metabolic status in mice and improved IR, a key feature of NAFLD. Furthermore, exposure of infants to antibiotics may have a long-term effect on the composition of the gut microbiota and increase the susceptibility to adiposity and NAFLD (44). Cho et al (45) generated a mouse model of obesity to evaluate changes in the capabilities and composition of the gut microbiota by administering antibiotics at subtherapeutic doses. The results indicated that marked changes in gut microbiota components and hepatic metabolism of cholesterol and lipids occurred, along with an increased production of colonic SCFA (45). Similar studies have been performed in humans. One study performed by Vrieze et al (46) indicated that allogenic gut microbiota infusion improved the peripheral insulin sensitivity. Furthermore, low-calorie diet therapy in obese individuals decreased the relative abundance of Firmicutes and increased the relative abundance of Bacteroidetes (33).
Although the changes in the gut microbiota were not entirely consistent among studies, it is definite that the gut microbiota contributes to the development of metabolic syndrome through multiple modes, which are discussed in the chapter below and summarized in Table I (47–80).
Mechanisms of gut microbiota dysbiosis in NAFLD/NASH
Gut-liver axis
The liver and intestine originate from the ventral foregut endoderm during the process of embryonic development; therefore, an intrinsic association exists between the anatomical and biological functions of the liver and intestine (47,48). Anatomically, the liver and intestine are interconnected through the portal vein, and 70–75% of the liver's blood supply comes from the intestine through the portal vein. At the same time, an array of bacteria, bacterial metabolites and environmental toxins, which are derived from the intestine, may reach the liver through the portal vein (49). In recent years, emerging evidence has indicated that dysfunction of the gut-liver axis, including mucosa permeability alteration, bacterial overgrowth and intestinal dysbiosis, significantly contribute to the development and progression of NAFLD (50). Therefore, the systemic endotoxin concentration, increased permeability of the intestinal epithelium and endogenous ethanol levels may reflect an abnormal gut-liver axis function in hepatic diseases. In addition, all of the above-mentioned factors may trigger the production of a cascade of cytokines, activating the uncontrolled immune response and causing the release of multiple inflammatory mediators (48,51).
Small intestinal bacterial overgrowth (SIBO)
SIBO is usually defined as the presence of >105 colony-forming units (CFU)/ml in duodenal aspirate cultures (52). Dyspepsia, abdominal discomfort, watery diarrhea, bloating, nutrient malabsorption, flatulence and hepatic steatosis are all clinical manifestations of SIBO (53,54). Accumulated evidence suggested that SIBO is common in NAFLD patients and has an important role in the pathogenesis of NAFLD. In 2001, Wigg et al (55) investigated SIBO in NASH patients and healthy controls. They reported that SIBO was observed in 50% of NASH patients and 22% of healthy controls, and the mean levels of tumor necrosis factor (TNF)-α in NASH patients and controls were 14.2 and 7.5 pg/ml, respectively. Ghoshal et al (56) determined the amount of intestinal bacteria using quantitative jejunal aspirate culture and the glucose hydrogen breath test, and the results indicated low-grade SIBO (≥103 CFU/ml) in NASH patients compared with that in controls. These results confirmed that patients with NASH have an increased prevalence of SIBO.
Various observational studies in NAFLD patients and experimental studies in animals have been performed to explore the detailed pathogenesis of SIBO in NAFLD. Wigg et al (55) and Boulangé et al (57) reported that SIBO increased intestinal permeability and endotoxin absorption, thereby leading to gut inflammation, dysmotility and various immunological changes in the gut, which may contribute to the development of liver injury. Shanab et al (58) proved that SIBO in patients with NASH was associated with enhanced hepatic release of interleukin-8 and expression of Toll-like receptor (TLR)4, contributing to the development and progression of NASH. Zucker rats were fed on a diet rich in LPS to simulate the endotoxin coming from the intestinal microbiota, and significantly higher levels of plasma insulin and glucose were observed after 24 weeks of feeding. These results suggested that LPS aggravated the development of IR (59). Gut-derived endogenous endotoxin, including LPS, may have an important role in the activation of Kupffer cells to produce cytokines, including TNF-α, which has been suggested to cause IR. IR increases the accumulation of hepatic TG by promoting peripheral lipolysis, TG synthesis and hepatic uptake of free FAs (59,60). From the above, it is apparent that SIBO participates in the pathogenesis of NAFLD by increasing intestinal permeability to induce inflammation and IR.
Microbial metabolites
Interactions among the gut microflora, the liver and the immune system trigger the inflammatory response pathway of the intestinal flora in the body (61). Under normal conditions, less intestinal bacterial products, including endotoxin, enter the portal circulation by permeating the normal intestinal barrier and reach the liver (62). Permeated endotoxin may be detected by pattern recognition receptors, including TLRs, which are located on Kupffer cells, and the Kupffer cells then eliminate the endotoxin in the liver (46,63). LPS is the major structural component of Gram-negative bacteria and the major component of endotoxin. LPS may be recognized by LPS-binding protein (LBP) in serum and is the major activator of the innate immune response (64). Ruiz et al (65) indicated that the serum levels of LBP were increased in obese patients with NASH compared to patients with simple steatosis (P<0.05), and the increased serum LBP level was correlated to an upregulated expression of TNF-α in the liver tissue. This result supported that endotoxin has a key role in the development of steatohepatitis in obese patients (65). Furthermore, TLR4-mutant mice induced by high fat and high fructose were more susceptible to steatohepatitis and overexpression of inflammation-associated factors than wild-type mice, which suggested that the TLR4 signaling pathway participates in the pro-inflammatory response in the liver (66). Of note, abnormal levels of adipokines and pro-inflammatory cytokines were observed in almost all NAFLD patients, and endoplasmic reticulum, mitochondria and cytokine-mediated oxidative stress were indicated to promote the development of NASH via the TLR signaling pathway (67–70). Thus, TLR antagonists may be effective therapeutic agents for NASH that are worthy of further study (71).
Effects of BAs
The hepatic load of BAs is derived from the intestine and has numerous functions, including the promotion of gastrointestinal absorption of fat and fat-soluble vitamins, inhibition of overgrowth of the intestinal microflora and gain of weight (72). Therefore, BAs have an important role in the development of NAFLD. In the colon, primary BAs are transformed into secondary BAs through various bacterial-mediated modifications, including deconjugation and dihydroxylation (73). Farnesoid X receptor (FXR), a nuclear receptor known to be involved in hepatic and intestinal BA metabolism, and Takeda G-protein-coupled receptor 5 have key roles in glucose and energy metabolism and are the most significant BA receptors in the liver (74,75). Sphingosine-1-phosphate (S1P) receptor 2/sphingosine kinase 2/S1P signaling lowers the hepatic lipid content by modulating the expression of nuclear receptors (including FXR) and proteins involved in lipid metabolism. The gut microbiota regulates the lipid and lipoprotein metabolism in an FXR-dependent manner, including acting on hepatic lipogenesis, as well as lipoprotein secretion, plasma clearance and intestinal cholesterol absorption. In addition, FXR reduces TG-rich lipoproteins through a variety of mechanisms. For instance, FXR reduces lipogenesis by repressing hepatic sterol regulatory element-binding protein (SREBP)1c expression in small heterodimer partner (SHP)-79- and fibroblast growth factor 15/19-dependent manners, reduces very low-density lipoprotein secretion by repressing microsomal TG transfer protein and apolipoprotein B gene expression, and stimulates intravascular lipolysis of TG-rich lipoproteins by enhancing lipoprotein lipase activity (74). Microbiota-associated BA deconjugation may accelerate fat synthesis by activating the intestinal FXR signaling pathway and blocking the hepatic FXR-SHP signaling pathway. FXR activation may prevent the progression of NAFLD, as it reduces steatosis by inhibiting lipogenesis, decreases chemically induced hepatitis and maintains the integrity of the intestinal barrier, thus protecting the liver from bacteria-derived inflammatory signals. As these factors were put together, downregulation of hepatic FXR receptor led to enhanced lipogenesis and suppressed FA β-oxidation, thereby promoting the development of NAFLD (76,77).
To explore the functional association between changes of the gut microbiota and the host's metabolic syndrome, Zhang et al (78) treated the HFD-fed mice with a potent FXR antagonist, Gly-β-muricholic acid. A marked improvement of obesity-associated metabolic disorders in the liver was observed after 8 weeks of treatment, which suggested that hepatic changes were associated with the significantly modulated gut microbiota (78). Janssen et al (76) reported that elevated BAs in plasma and liver tissue are potentially linked to changes of the gut microbial composition, NAFLD-associated hepatic inflammation and fibrosis in a mouse model, suggesting a possible mechanism through which the gut microbiota promotes the progression of NAFLD by interfering with the normal metabolism of BAs. In summary, gut microbiota dysbiosis promotes NAFLD, likely through regulating BA metabolism by the intestinal flora to target the FXR. In consideration of the key role of FXR in NAFLD, FXR has been identified as a promising pharmacological target for the treatment of abnormal liver BA and lipid metabolism diseases, including cholestasis and NAFLD (79,80).
Gut microbiota-based treatments for NAFLD/NASH
Probiotics, prebiotics and synbiotics supplementation
Studies on animal models have provided evidence that probiotics, prebiotics and synbiotics possess significant modulatory effects on the intestinal microbiota. Oral administration of probiotics and prebiotics, as well as supplementation of synbiotics, contributed to the treatment of NAFLD by improving the abnormal lipid metabolism and gut microbiota dysbiosis (81). Alves et al (82) reported that prebiotic and synbiotic supplementation in rats improved hypercholesterolemia-associated hepatic changes by regulating the expression of genes involved in β-oxidation [peroxisome proliferator activated receptor-α (PPAR-α) and carnitine palmitoyltransferase 1 (CPT-1)] and lipogenesis [sterol regulatory element-binding protein 1c (SREBP-1c), fatty acid synthase (FAS) and malic enzyme (ME)]. Another study indicated that supplementation with probiotics (Lactobacillus and Bifidobacterium) attenuated hepatic fat accumulation in rats receiving a high-fat diet (HFD), but no significant differences of intestinal permeability were observed in probiotics group compared to control (83). Raso et al (84) indicated that a HFD induced overexpression of TLR4 and CD14 at the mRNA and protein levels in a rat model. After the rats were treated with synbiotics, hepatic inflammation and IR were improved and the amount of Gram-negative Enterobacteriales and Escherichia coli in colonic mucosa was reduced. In pediatric patients with obesity NAFL and NASH, Nobili et al (85) observed that the amount of Bifidobacterium spp. was significantly decreased compared with that in a control group, suggesting that Bifidobacterium spp. may possess a protective effect against obesity and NAFLD. All of these results highlight that understanding the detailed mechanisms of gut microbiota in NAFLD and identification of beneficial bacteria are important for the exploration of microbiota-targeted therapeutic methods for NAFLD.
Fecal microbiota transplantation (FMT)
FMT, a method used to re-populate the gut microbiota of patients with a healthy intestinal flora, is currently being tested as a promising treatment method for various diseases (86–88). Application of FMT in the treatment of NAFLD/NASH has been widely explored. Early studies using mouse models by Zhou et al (89) and Le Roy et al (90) indicated that intrahepatic lipid accumulation, IR and increased pro-inflammatory cytokines were improved by FMT. In a randomized controlled trial, patients with metabolic syndrome received the gut microbiota from lean healthy Caucasian males via duodenal tube. Increased insulin sensitivity (median rate of glucose disappearance, 26.2 vs. 45.3 mol/kg/min; P<0.05) and gut microbial diversity (178±62 vs. 234±40 species; P<0.05) of recipients was observed after 6 weeks of FMT (46). These studies suggest that FMI may be developed as a potential therapeutic method to regulate lipid and glucose metabolism in NAFLD patients. However, the optimal FMT method and bacterial species for transplantation require to be further identified.
Anti-diabetic drugs
IR is one of the major features of T2DM (91). Over the past decade, IR and NAFLD/NASH have been widely elucidated. Patients with abundant obesity-associated rather than genetics-associated NAFLD present with severe IR. Once visceral adipose tissue becomes insufficient to store excess fat, the latter start to ectopically accumulate in key sensitive organs, including the liver, hence causing systemic IR (92). Excessive deposition of TG in the liver may promote lipid peroxidation and accumulation of reactive oxygen species, resulting in a variety of cellular stimuli with subsequent inflammatory response, hepatocellular injury and fibrosis. IR accelerated the above processes that led to the development of NAFLD to NASH, cirrhosis and even HCC. Therefore, novel anti-diabetic drugs may serve as significant candidates for the treatment of NAFLD/NASH by improving IR and gut microbiota dysbiosis (Table II) (93,94).
Metformin is currently the first-line drug against T2DM on account of its good effect to improve insulin sensitivity and reduce blood glucose (95). Previous studies suggested that metformin possesses promising potential in terms of gut-based pharmacology, including the alteration of gut microbiota and BA recirculation (96,97). Shin et al (98) reported that treatment with metformin for 6 weeks modulated the gut microbiota composition in mice with HFD-induced obesity. Lee and Ko (99) reported that metformin improved metabolic disorders, including hyperglycemia, obesity and hypercholesterolemia in mice receiving a HFD, and the composition of the gut microbiota was also changed after metformin treatment. Similarly, another anti-diabetic drug, the α-glucosidase inhibitor voglibose, reversed the increased ratio of Firmicutes vs. Bacteroidetes in the gut microbiota of mice on a HFD (100). In Table II, the detailed effects of anti-diabetic drugs on the composition of the gut microbiota in NAFLD-associated diseases reported by most of the studies published to date are summarized (98,99,101–110). While the detailed mechanisms of action of anti-diabetic drugs on the gut microbiota remain elusive, those drugs have been proven to be a promising therapeutic option to control NAFLD/NASH.
Conclusions
More attention should be paid to the early diagnosis and treatment of NAFLD as the most prevalent chronic liver disease worldwide. For the management of NAFLD as a multi-factorial disease, a healthy lifestyle and reasonable diet should be implemented. The gut microbiota significantly contributes to the development and progression of NAFLD, and a vast amount of evidence has been provided to shed light on the pathogenesis of the gut microbiota in NAFLD (Fig. 1). Although certain changes in the gut microbiota observed by previous studies were not specific and the compositional alterations were not absolutely consistent, it is apparent that gut microbiota-based treatment methods for NAFLD are promising. Future studies should focus on elucidating the detailed mechanisms of the gut microbiota in NAFLD and determining NAFLD-associated microbial species to thus pave the road for the development of more specific medicines and effective therapeutic methods for NAFLD based on the gut microbiota.
Acknowledgements
Not applicable.
Funding
This study was supported by Grants from the National Natural Science Foundation of China (grant no. 31770837) and the Qingdao People's Livelihood Science and Technology Plan (grant no. 18-6-1-68-nsh).
Availability of data and materials
Not applicable.
Authors' contributions
QL, SSL, LZC, ZZZ, SXD and QJD contributed to the acquisition, analysis and systematization of data. QL and SSL wrote the manuscript. The manuscript was critically revised for important intellectual content by YNX and SYX. YNX and SYX conceived the present study. All authors read and approved the final version of the manuscript.
Ethics approval and consent to participate
Not applicable.
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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