HSP27 regulates TGF-β mediated lung fibroblast differentiation through the Smad3 and ERK pathways

  • Authors:
    • Gang Wang
    • Hao Jiao
    • Jun-Nian Zheng
    • Xia Sun
  • View Affiliations

  • Published online on: November 28, 2016     https://doi.org/10.3892/ijmm.2016.2813
  • Pages:183-190
0

Abstract

Idiopathic pulmonary fibrosis (IPF) is a chronic lethal interstitial lung disease with unknown etiology. Recent studies have indicated that heat-shock protein 27 (HSP27) contributes to the pathogenesis of IPF through the regulation of epithelial-mesenchymal transition (EMT). However, the expression and role of HSP27 in fibroblasts during pulmonary fibrogenesis has not been investigated to date, at least to the best of our knowledge. In this study, we examined the expression of HSP27 in fibrotic lung tissue and fibroblasts from bleomycin (BLM)-challenged mice and human lung fibroblasts treated with transforming growth factor-β (TGF-β). The results revealed that the expression of HSP27 was significantly increased in fibrotic lung tissue and fibroblasts from BLM-challenged mice. In vitro, TGF-β stimulated HSP27 expression in and the differentiation of human lung fibroblasts. The knockdown of Smad3 expression or nuclear factor-κB p65 subunit attenuated the TGF-β-induced increase in HSP27 expression and the differentiation of human lung fibroblasts. In addition, the knockdown of HSP27 expression attenuated the TGF-β-induced activation of ERK and Smad3, and inhibited the differentiation of human lung fibroblasts. On the whole, the findings of our study demonstrate that HSP27 expression is upregulated in lung fibroblasts during pulmonary fibrosis, and subsequently, HSP27 modulates lung fibroblast differentiation through the Smad3 and ERK pathways.

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January 2017
Volume 39 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
Ranked #21/128 Medicine Research and Experimental
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APA
Wang, G., Jiao, H., Zheng, J., & Sun, X. (2017). HSP27 regulates TGF-β mediated lung fibroblast differentiation through the Smad3 and ERK pathways. International Journal of Molecular Medicine, 39, 183-190. https://doi.org/10.3892/ijmm.2016.2813
MLA
Wang, G., Jiao, H., Zheng, J., Sun, X."HSP27 regulates TGF-β mediated lung fibroblast differentiation through the Smad3 and ERK pathways". International Journal of Molecular Medicine 39.1 (2017): 183-190.
Chicago
Wang, G., Jiao, H., Zheng, J., Sun, X."HSP27 regulates TGF-β mediated lung fibroblast differentiation through the Smad3 and ERK pathways". International Journal of Molecular Medicine 39, no. 1 (2017): 183-190. https://doi.org/10.3892/ijmm.2016.2813