Open Access

Notoginsenoside R1 attenuates glucose-induced podocyte injury via the inhibition of apoptosis and the activation of autophagy through the PI3K/Akt/mTOR signaling pathway

  • Authors:
    • Guodong Huang
    • Bingyu Zou
    • Jianzhen Lv
    • Tongyu Li
    • Guoli Huai
    • Shaowei Xiang
    • Shilong Lu
    • Huan Luo
    • Yaping Zhang
    • Yi Jin
    • Yi Wang
  • View Affiliations

  • Published online on: Friday, January 20, 2017
  • Pages:559-568 DOI: 10.3892/ijmm.2017.2864
0

Abstract

Injury to terminally differentiated podocytes contributes ignificantly to proteinuria and glomerulosclerosis. The aim of this study was to examine the protective effects of notoginsenoside R1 (NR1) on the maintenance of podocyte number and foot process architecture via the inhibition of apoptosis, the induction of autophagy and the maintenance pf podocyte biology in target cells. The effects of NR1 on conditionally immortalized human podocytes under high glucose conditions were evaluated by determining the percentage apoptosis, the percentage autophagy and the expression levels of slit diaphragm proteins. Our results revealed that NR1 protected the podocytes against high glucose-induced injury by decreasing apoptosis, increasing autophagy and by promoting cytoskeletal recovery. The phosphoinositide 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling pathway was further investigated in order to elucidate the mechanisms responsible for the protective effects of NR1 on podocytes. Our data indicated that treatment with NR increased the phosphorylation levels of PI3K, Akt and mTOR, leading to the activation of the PI3K/Akt/mTOR signaling pathway in podocytes. To the best of our knowledge, this is the first in vitro study to demonstrate that NR1 protects podocytes by activating the PI3K/Akt/mTOR pathway.

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Journal Cover

March 2017
Volume 39 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

2015 Impact Factor: 2.348
Ranked #22/123 Medicine Research and Experimental
(total number of cites)

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APA
Huang, G., Zou, B., Lv, J., Li, T., Huai, G., Xiang, S. ... Wang, Y. (2017). Notoginsenoside R1 attenuates glucose-induced podocyte injury via the inhibition of apoptosis and the activation of autophagy through the PI3K/Akt/mTOR signaling pathway. International Journal of Molecular Medicine, 39, 559-568. http://dx.doi.org/10.3892/ijmm.2017.2864
MLA
Huang, G., Zou, B., Lv, J., Li, T., Huai, G., Xiang, S., Lu, S., Luo, H., Zhang, Y., Jin, Y., Wang, Y."Notoginsenoside R1 attenuates glucose-induced podocyte injury via the inhibition of apoptosis and the activation of autophagy through the PI3K/Akt/mTOR signaling pathway". International Journal of Molecular Medicine 39.3 (2017): 559-568.
Chicago
Huang, G., Zou, B., Lv, J., Li, T., Huai, G., Xiang, S., Lu, S., Luo, H., Zhang, Y., Jin, Y., Wang, Y."Notoginsenoside R1 attenuates glucose-induced podocyte injury via the inhibition of apoptosis and the activation of autophagy through the PI3K/Akt/mTOR signaling pathway". International Journal of Molecular Medicine 39, no. 3 (2017): 559-568. http://dx.doi.org/10.3892/ijmm.2017.2864