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Protease activity induced by nicotine in human cells.

  • Authors:
    • X L Wang
    • K Kita
    • S Takahashi
    • R Hasegawa
    • X J Chi
    • H Yamamori
    • N Nakajima
    • H Yokoe
    • H Tanzawa
    • N Suzuki

  • View Affiliations

  • Published online on: November 1, 1999     https://doi.org/10.3892/ijmm.4.5.537
  • Pages: 537-577
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
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Abstract

Nicotine has a wide range of biological effects, and proteases have been extensively studied for their biological roles in living creatures. The aim of this study is to determine whether nicotine can induce proteolytic protease activity in cultures of various human cell lines. Plasminogen activator-like fibrinolytic protease activity, using 125I-fibrin as substrate in the presence of plasminogen, was estimated in cells with and without nicotine treatment. Among 16 cell lines tested, APr-1 cells were found to have the highest induced protease activity. Partial purification of the proteases was carried out by high performance liquid chromatography gel filtration on TSKG2000SW. Protease inhibitor tests indicated that the proteases induced by nicotine are serine proteases.

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Journal Cover

Nov 1999
Volume 4 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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  • Journal Metrics
  • Impact Factor: 5.7
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 12.3
  • CiteScore Rank: Q1: #28/347 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 1.022
  • SCImago Journal Rank (SJR): 1.167
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Spandidos Publications style
Wang X, Kita K, Takahashi S, Hasegawa R, Chi X, Yamamori H, Nakajima N, Yokoe H, Tanzawa H, Suzuki N, Suzuki N, et al: Protease activity induced by nicotine in human cells.. Int J Mol Med 4: 537-577, 1999.
APA
Wang, X., Kita, K., Takahashi, S., Hasegawa, R., Chi, X., Yamamori, H. ... Suzuki, N. (1999). Protease activity induced by nicotine in human cells.. International Journal of Molecular Medicine, 4, 537-577. https://doi.org/10.3892/ijmm.4.5.537
MLA
Wang, X., Kita, K., Takahashi, S., Hasegawa, R., Chi, X., Yamamori, H., Nakajima, N., Yokoe, H., Tanzawa, H., Suzuki, N."Protease activity induced by nicotine in human cells.". International Journal of Molecular Medicine 4.5 (1999): 537-577.
Chicago
Wang, X., Kita, K., Takahashi, S., Hasegawa, R., Chi, X., Yamamori, H., Nakajima, N., Yokoe, H., Tanzawa, H., Suzuki, N."Protease activity induced by nicotine in human cells.". International Journal of Molecular Medicine 4, no. 5 (1999): 537-577. https://doi.org/10.3892/ijmm.4.5.537