Induction of apoptosis by dietary polyunsaturated fatty acids in human leukemic cells is not associated with DNA fragmentation.

  • Authors:
    • L C Chiu
    • J M Wan
    • V E Ooi
  • View Affiliations

  • Published online on: October 1, 2000     https://doi.org/10.3892/ijo.17.4.789
  • Pages: 789-885
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Abstract

Apoptosis is important in anticancer strategy. In this study, bivariate annexin V/PI flow cytometry showed that dietary polyunsaturated fatty acids, arachidonic acid (AA) and eicosapentaenoic acid (EPA), induced apoptosis in human leukemic HL-60 but not K-562 cells. Results from DNA-PI flow cytometry and TUNEL flow cytometry illustrated that neither AA nor EPA induced DNA fragmentation in the leukemic cells. These findings suggested that the AA- and EPA-induced apoptosis might not associate with endonucleases activation, and DNA fragmentation could not be used as a sole criterion to identify apoptotic cells.

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Oct 2000
Volume 17 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Chiu L, Wan J and Ooi V: Induction of apoptosis by dietary polyunsaturated fatty acids in human leukemic cells is not associated with DNA fragmentation.. Int J Oncol 17: 789-885, 2000.
APA
Chiu, L., Wan, J., & Ooi, V. (2000). Induction of apoptosis by dietary polyunsaturated fatty acids in human leukemic cells is not associated with DNA fragmentation.. International Journal of Oncology, 17, 789-885. https://doi.org/10.3892/ijo.17.4.789
MLA
Chiu, L., Wan, J., Ooi, V."Induction of apoptosis by dietary polyunsaturated fatty acids in human leukemic cells is not associated with DNA fragmentation.". International Journal of Oncology 17.4 (2000): 789-885.
Chicago
Chiu, L., Wan, J., Ooi, V."Induction of apoptosis by dietary polyunsaturated fatty acids in human leukemic cells is not associated with DNA fragmentation.". International Journal of Oncology 17, no. 4 (2000): 789-885. https://doi.org/10.3892/ijo.17.4.789