A case of distal extrahepatic cholangiocarcinoma with two positive resection margins

  • Authors:
    • Wayne A. Warner
    • Wesley Ramcharan
    • Dave Harnanan
    • Srikanth Umakanthan
    • Ravi Maharaj
  • View Affiliations

  • Published online on: September 22, 2016     https://doi.org/10.3892/ol.2016.5174
  • Pages:4075-4079
0

Abstract

Cholangiocarcinoma is an uncommon primary malignancy of the biliary tract that is challenging to diagnose and treat effectively due to its relatively silent and late clinical presentation. The present study reports a case of a 60‑year‑old male with distal extrahepatic cholangiocarcinoma with a 3‑week history of painless obstructive jaundice symptoms and subjective weight loss. Imaging revealed an obstructing lesion in the common bile duct, just distal to the entrance of the cystic duct. Pathology revealed moderately differentiated cholangiocarcinoma with two positive proximal resection margins. The two positive resection margins presented a challenge during surgery and points to an urgent need for further studies to better illuminate diagnostic and therapeutic options for patients with similar clinicopathological presentation.

Introduction

Cholangiocarcinoma (CCA), an extremely rare and highly aggressive malignant tumor was first described in 1957 (1). They are cancers of the epithelial cells in the extrahepatic or intrahepatic biliary tree of the bile duct from the bile ductules to the Ampulla of Vatar (24). CCA is often diagnosed in patients >60 years and the prognosis is poor in the majority of cases (5,6). Furthermore, CCA is often difficult to diagnose and treat due to its growth pattern, silent nature (wherein its symptoms go unnoticed by the patient until the advanced stage), anatomical location, non-specific clinical presentation and limited clinical approaches. CCA is the second most common primary hepatic tumor globally, accounting for 3% of all gastrointestinal tumors, though that rate is increasing (7,8). Worldwide, the highest CCA rates have been reported in Eastern Asia, particularly Thailand, where its high incidence is attributable to liver fluke infection (9). To the best of our knowledge, case reports of distal extrahepatic CCA with two positive resection margins are rare. This is the first reported CCA case from the Caribbean (30 island nations, population, 39.8 million) and more specifically, Trinidad and Tobago (population, 1.3 million).

Case report

The present study presents a case of a distal extrahepatic cholangiocarcinoma with its imaging and clinicopathological details, as well as the epidemiology of CCA in Trinidad and Tobago. A 60-year-old male non-smoker of African and Indian ethnicity, with no previously known medical conditions presented to the Eric Williams Medical Sciences Complex, Champ Fleurs (Trinidad and Tobago) with a 3-week history of painless obstructive jaundice symptoms and subjective weight loss. The patient has a family history of breast, prostate and lung cancer (Fig. 1). The patient's mother and an aunt succumbed to lung and breast cancer, respectively; the patient's father has prostate cancer and several of his uncles have prostate and/or colon cancer.

On examination, the patient had icterus and a palpable gallbladder. Complete blood count, renal function and international normalized ratio tests were normal, but his liver function tests were abnormal: Total bilirubin, 9.1 mg/dl and bilirubin, 4.5 mg/dl. Computed tomography (CT) of the chest, abdomen and pelvis scan revealed dilated intra-hepatic ducts, gallbladder, cystic duct and common hepatic duct, just distal to entrance of cystic duct (Fig. 2). Magnetic resonance cholangiopancreatography (MRCP) revealed an obstructing lesion in the common bile duct with proximal extent just distal to the cystic duct and distal extent just superior to the upper border of the pancreas (Fig. 3).

An initial bile duct excision was made 1 cm proximal to the tumor and distally down to the upper border of the pancreas. Kocherization of the duodenum allowed exposure of the retroperitoneum and visualization of the neighboring structures. A lymph node at station 8A was excised. Following bile duct excision, imprint cytology demonstrated that both superior and inferior margins were invaded by the tumor. The proximal bile duct was re-excised to the hepatic ductal confluence, however, this margin was also positive. A pancreaticoduodenectomy (Whipple procedure) was performed to gain a clear distal margin but no further resection was done on the proximal bile duct, due to the lack of intra-operative imprint cytology at that time leaving us unable to perform a right vs. left hepatectomy, as we were unsure which hepatic duct was involved. The MRCP demonstrated it was free of tumor and macroscopically both ducts appeared normal.

Macroscopically, the cut surface of common bile duct revealed an ulcero-proliferative lesion (3.0×2.0×1.5 cm), 0.2 cm from the proximal bile duct margin and 1.0 cm from the distal bile duct margin. Histology revealed T2N1M0 moderately differentiated CCA with a positive proximal resection margin and metastatic deposits in station 8A lymph node along with peri-neural invasion (Fig. 4). Post-operatively, the patient had bile noted from the drains with resolution after two weeks. Six-months postoperative, the patient is receiving adjuvant chemotherapy (capecitabine) and has completed 30 cycles of external beam radiotherapy to the porta hepatis due to the positive proximal margin. At six months follow up, the patient showed small hepatic metastasis upon CT screening.

Discussion

In Trinidad and Tobago there were 65 reported CCA cases from 1995–2007, accounting for 0.41% of all incident cancer cases (personal communication from the Trinidad and Tobago National Cancer Registry). There was a slight male predilection (56.9%) and the overall average incident age was 60–69 years. The present case report, in addition to the majority of cases (66.1%) in TT originate in the extrahepatic bile duct, with the remaining cases occurring in the biliary track NOS (13.8%) followed by the Ampulla of Vater (0.2%). Forty-six percent of cases were diagnosed at autopsy and the majority of the other cases were diagnosed by histology of the primary tumor. The majority of cases occurred in nationals of African (63.1%), Indian (29.2%) then Mixed (African-Indian) ancestry (7.7%). This contrasts with data from the USA, where Hispanics and Asians have higher incident rates compared to African Americans (7).

The general risk factors for cholangiocarcinoma are primary sclerosing cholangitis (PSC), recurrent pyogenic cholangitis, hepatolithiasis, primary biliary cirrhosis, cholelithiasis, Asian liver flukes, biliary malformation (Caroli's disease, choledochal cysts), toxins (thorotrast, dioxin, polyvinyl chloride and heavy alcohol use), and viral infection (HIV, hepatitis B and hepatitis C and EBV) (1014). There was no evidence that our patient was exposed to these risk factors. In fact, most CCA cases like the one presented in the current study arise de novo (12).

Clinical management of these cases is challenging and requires a multimodality approach. While surgical resection is an optimal treatment approach, many patients are not candidates as the disease is often diagnosed at an advanced stage given that diagnostic symptoms like night sweats, malaise, jaundice, abdominal pain and cachexia are not uniquely informative (15). Another compounding factor is the possibility of metastatic spread to the hepatic vasculature, regional lymph nodes, lung, bones (especially vertebrae), adrenal system and brain.

According to the 8th edition of the Union for International Cancer Control-American Joint Committee on Cancer (UICC-AJCC) classification there are two divisions of extrahepatic cholangiocarcinoma-distal and perihilar. For distal extrahepatic CCA, tumor depth invasion, number of lymph node metastases, perineural, microscopic vascular invasion, R0 resection and pancreatic invasion are reported to be predictors of long term survival (1623). A single positive bile duct resection margin is usually correlated with increased risk of recurrence and poor prognosis, as is a positive lymph node (24,25). A recent study that examined 27 patients with distal bile duct cancer who underwent pancreatoduodenectomy with extended lymphadenectomy identified that factors for improved survival included up to two positive nodes, negative resection margins, and clinical administration of postoperative adjuvant chemotherapy (21).

Generally the prognosis for CCA is poor with 5-year survival of 5–10%, and a median survival of 3–6 months if unresectable (13,26). Post surgery survival time depends on a thorough resection with negative resection margins. It is well established that recurrences occur in 60–80% of patients within two years after the initial surgery (27). In the present case, given that the regional lymph nodes were positive our challenge was deciding how far to resect. We had to weigh the probability of local vs. distant recurrence and the morbidity and potential mortality of trying to gain a R0 proximal margin. It was ultimately decided that the probability of distant recurrence outweighed that of local recurrence.

Adjuvant therapy is often considered as part of the clinical management for post operative CCA where there are positive margins given the high rate of recurrence in these circumstances (28). Studies have reported survival benefits for chemoradiotherapy with incompletely resected tumors (3,29). A recent study looking at chemoradiation with 5-fluorouracil (5-FU) and external beam irradiation reported improved survival for distal tumors particularly in patients with histologically positive resection (30,31). It has been reported that gemcitabine-based approaches have better survival outcomes than 5-FU and should be considered as part of the clinical intervention (13). A recent report demonstrated that a low bilirubin level <10 mg/dl and chemotherapy administration are independent predictors associated with better survival (32).

Despite the genetic heterogeneity of CCA, perturbation of the RAS-MAPK pathway has been increasingly viewed as a potential signature genetic aberration in CCA (3337). In Trinidad and Tobago, CCA provides an opportunity to integrate epidemiological data, genomics, and novel clinical approaches to better screen and treat CCA cases (38). One such schema integrates diagnostic tests, and identification of targetable cancer driver pathways leading to personalized targeted therapy (33,34).

In conclusion, the present case demonstrates clearly that intra-operative assessment of margins in resection of CCA are paramount. Aggressive surgical resection followed by adjuvant therapy shows a definitive survival advantage. This case presented challenges given the difficulties to obtain a correct preoperative diagnosis, and to achieve R0 margins. Further studies are required to identify modalities to better diagnose and clinically intervene in these cases.

Acknowledgements

The authors of the present study appreciate the contributions of the Department of Pathology, Eric Williams Medical Sciences Complex, Trinidad and the assistance of Krishna Vyas, Washington University, St. Louis, USA with figure preparation. WAW was supported by Washington University School of Medicine-St. Louis (grant no., GSAS/CGFP Fund 94028C).

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November 2016
Volume 12 Issue 5

Print ISSN: 1792-1074
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APA
Warner, W.A., Ramcharan, W., Harnanan, D., Umakanthan, S., & Maharaj, R. (2016). A case of distal extrahepatic cholangiocarcinoma with two positive resection margins. Oncology Letters, 12, 4075-4079. https://doi.org/10.3892/ol.2016.5174
MLA
Warner, W. A., Ramcharan, W., Harnanan, D., Umakanthan, S., Maharaj, R."A case of distal extrahepatic cholangiocarcinoma with two positive resection margins". Oncology Letters 12.5 (2016): 4075-4079.
Chicago
Warner, W. A., Ramcharan, W., Harnanan, D., Umakanthan, S., Maharaj, R."A case of distal extrahepatic cholangiocarcinoma with two positive resection margins". Oncology Letters 12, no. 5 (2016): 4075-4079. https://doi.org/10.3892/ol.2016.5174