Open Access

The role of cadherin-11 in microcystin-LR-induced migration and invasion in colorectal carcinoma cells

  • Authors:
    • Qiangqiang Zhu
    • Zhen Wang
    • Lihua Zhou
    • Yan Ren
    • Ying Gong
    • Wei Qin
    • Lin Bai
    • Jun Hu
    • Ting Wang
  • View Affiliations

  • Published online on: November 21, 2017     https://doi.org/10.3892/ol.2017.7458
  • Pages:1417-1422
  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to explore whether microcystin‑LR (MC‑LR; a well‑known cyanobacterial toxin produced in eutrophic lakes or reservoirs) induced tumor progression by activating cadherin‑11(CDH11). A previous tumor metastasis PCR array demonstrated that MC‑LR exposure resulted in a significant increase in the expression of CDH11. In the present study, to confirm the effect of the MC‑LR treatment on CDH11 expression, HT‑29 cell migration and invasion following MC‑LR treatment were tested by Transwell assays, and protein levels of CDH11 were tested by immunofluorescence and western blot analysis. The results demonstrated that MC‑LR activated CDH11 expression in addition to cell migration and invasion in HT‑29 cells. To further investigate the association between MC‑LR‑induced CDH11 upregulation, and higher motility and invasiveness in HT‑29 cells, knockdown of CDH11 using small interfering RNA (siRNA) in HT‑29 cells was performed. Subsequent Transwell assays confirmed that MC‑LR‑induced enhancement of migration and invasion was significantly decreased following CDH11 knockdown by CDH11‑siRNA in HT‑29 cells. The results from the present study indicate that MC‑LR may act as a CDH11 activator to promote HT‑29 cell migration and invasion.

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February 2018
Volume 15 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Zhu, Q., Wang, Z., Zhou, L., Ren, Y., Gong, Y., Qin, W. ... Wang, T. (2018). The role of cadherin-11 in microcystin-LR-induced migration and invasion in colorectal carcinoma cells. Oncology Letters, 15, 1417-1422. https://doi.org/10.3892/ol.2017.7458
MLA
Zhu, Q., Wang, Z., Zhou, L., Ren, Y., Gong, Y., Qin, W., Bai, L., Hu, J., Wang, T."The role of cadherin-11 in microcystin-LR-induced migration and invasion in colorectal carcinoma cells". Oncology Letters 15.2 (2018): 1417-1422.
Chicago
Zhu, Q., Wang, Z., Zhou, L., Ren, Y., Gong, Y., Qin, W., Bai, L., Hu, J., Wang, T."The role of cadherin-11 in microcystin-LR-induced migration and invasion in colorectal carcinoma cells". Oncology Letters 15, no. 2 (2018): 1417-1422. https://doi.org/10.3892/ol.2017.7458