Recruitment and significance of Th22 cells and Th17 cells in malignant ascites
- Authors:
- Published online on: August 16, 2018 https://doi.org/10.3892/ol.2018.9316
- Pages: 5389-5397
Abstract
Introduction
Malignant ascites (MA) is a pathological condition due to tumor cell invasion and metastasis to the abdominal cavity. The appearance of MA indicates progression of cancer, poor quality of life and decreased life expectancy for patients (1). Hepatocellular carcinoma (HCC), gastric cancer and ovarian cancer typically lead to the occurrence of MA (2). Similar to other types of immune microenvironment, MA contains a large number of lymphocytes, particularly cluster of differentiation (CD)4+ T helper (Th) lymphocytes, tumor cells and other types of immune cell (3). CD4+ T cells may inhibit the T cell response to exogenous- and auto-antibodies to maintain their own composition of immune tolerance in the tumor and prevent the body mounting an immune response. In addition, CD4+ T cells participate in tumor growth and metastasis via the secretion of a variety of soluble inflammatory, and immune factors (4).
Th22 cells have been identified as a novel CD4+ T cell subset, and primarily secrete interleukin (IL-)22, IL-6 and IL-1β, but not IL-17 or interferon (IFN-)γ (5). IL-22 is the effector cytokine of Th22 cells and belongs to the IL-10 cytokine family (6). Th22 cells and IL-22 has been implicated in the pathogenesis of liver cancer (7). In addition, the overexpression of IL-22 in primary tumor tissue and malignant pleural effusion may be involved in the occurrence, and progression of lung cancer (8). Furthermore, Th17 cells are a novel subset of CD4+ T cells and produce IL-17, but not IFN-γ (9). Th17 cells serve a dominant function in a variety of autoimmune diseases and cancers, including rheumatoid arthritis and gastric cancer (10,11). An increased number of Th17 cells have been observed in patients with lung cancer exhibiting malignant pleural effusion (12,13). In a previous study of multiple sclerosis lesions and lung inflammation, it was demonstrated that IL-22 and IL-17 increased vascular endothelial permeability (14,15). However, studies regarding the function of Th22 and Th17 cells in MA is limited, and the biological function of Th22 and Th17 cells in MA remains unknown. Therefore, the aim of the present study was to investigate the distribution and the phenotypic characteristics of Th22 and Th17 cells in patients with HCC. The present study additionally aimed to determine the molecular mechanism underlying the recruitment of peritoneal Th22 and Th17 cells into MA in patients with HCC. Finally, the prognostic value of peritoneal Th17 and Th22 cells was examined in patients with MA.
Patients and methods
Patients
The present study was approved by the Ethics Committee of the First Affiliated Hospital of Guangxi Medical University for human studies and all patients provided written informed consent. MA samples were selected from 26 patients with newly diagnosed HCC with MA (AJCC stage IV) at The First Affiliated Hospital of Guangxi Medical University (Nanning, China) between February 2013 and December 2015. The mean age of the patients was 53.7±7.6 years. In addition, 15 healthy volunteers as controls (mean age, 50.9±5.5 years) during the same period were recruited in the present study. A diagnosis of MA was established by the demonstration of malignant cells in peritoneal effusion and/or in closed peritoneal biopsy specimen. The patients were excluded if they had received any invasive procedures directed into the peritoneal cavity or if any abdominal trauma had occurred within 3 months prior to hospitalization. No patients received antitumor treatment and or glucocorticoid or other non-steroidal anti-inflammatory drugs prior to participating in the present study. The clinical information for the MA patients and healthy controls are summarized in Table I.
Sample collection and processing
Within 24 h of hospitalization, between 400 and 800 ml MA samples were selected from each patient and placed in heparinization tubes, using a standard abdominocentesis technique, and 10 mm peripheral blood was drawn. The MA specimens were immersed in ice immediately and subsequently centrifuged at 800 × g for 10 min at 4°C. The supernatants of MA and plasma were frozen at −80°C immediately for use in subsequent experiments. The cell pellets of MA were resuspended in PBS and mononuclear cells were isolated using Ficoll-Hypaque gradient centrifugation (Solarbio, Beijing, China) at 4°C, 800 × g to determine the T cell subsets within 1 h.
Flow cytometry
Following surface or intracellular staining with anti-human-specific antibodies (Abs) conjugated with Peridinin-Chlorophyll-protein (PerCP)-cysteine (cy)5.5, allophycocyanin (APC), phycoerythrin (PE), Alexa Fluor (AF)647, AF488 or fluorescein isothiocyanate (FITC). The expression of markers on T cells from MA and peripheral blood were determined using flow cytometry as previously described (12). The human Abs against anti-CD4, -IL-22, -IL-17A and -IFN-γ were purchased from BD Biosciences (Franklin Lakes, NJ, USA; cat. nos. 561844, 12-7229-41, 559502 and 551221, respectively). Human antibodies against -C-C motif chemokine receptor (CCR)4, -CCR6 and -CCR10 were purchased from Thermo Fisher Scientific, Inc. (Waltham, MA, USA; cat. nos. PA5-11866, CSB-E04660h and 720308). The anti-CD4, -IL-22, -IL-17A and -IFN-γ were diluted at 1:200, and the CCR4, -CCR6 and -CCR10 were diluted at 1:500. Intracellular staining for IL-22 or IL-17 was performed on T cells stimulated with phorbol myristate acetate (50 ng/ml; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) and ionomycin (1 µg/ml; Sigma-Aldrich; Merck KGaA) in the presence of GolgiStop (1.7 ug/ml; BD Biosciences) at 4°C for 5 h, and subsequently stained with PerCP-cy5.5-CD4, PE-CCR4, FITC-CCR6 or PE-CCR10 at room temperature in the dark for 30 min. Cells were washed twice with staining buffer (1 ml/wash for tubes) and pellet by centrifugation (250 × g). Cells were resuspended in 250 µl tubes of Fixation/Permeabilization solution (BD Biosciences) for 20 min at 4°C. Cells were washed two times in 1X BD Perm/Wash™ buffer (1 ml/wash for staining in tubes). The cells were stained with APC or PE-IL-22, AF647-IL-17A and AF488-IFN-γ at 4°C for 30 min, following fixation (100 µl of Fixation Buffer and pulse vortex) at 4°C in the dark room for 20 min. The permeabilization was performed according to the manufacturer's protocol. Mouse immunoglobulin G irrelevant isotype control (BD Biosciences; cat. no. MAB0032; dilution 1:100) served as isotype control. Stained cells were washed 2 times with 1× BD Perm/Wash™ buffer (1 ml/tubes), resuspend in Staining Buffer and subsequently analyzed by flow cytometric analysis using BD FCSDiva Software and FCS Epress 4 software (De Novo Software, Los Angeles, CA).
ELISA
MA and peripheral blood specimens were collected into heparinization tubes. The cell-free supernatants of MA and plasma were obtained from all subjects by centrifugation 800 × g for 10 min at room temperature and stored at −80°C immediately for determination of cytokines. The concentration of cytokines IL-22 and IL-17, chemokines C-C motif chemokine ligand (CCL)20, CCL22 and CCL27 in MA, and plasma were determined using an ELISA kit (cat. no. CSB-E09125h; R&D Systems, Inc., minneapolis, MN, USA,) in accordance with the manufacturer's protocol (lower detection limit 1 pg/ml; eBioscience; Thermo Fisher Scientific, Inc.). All samples were assayed in duplicate.
Chemotaxis assay of Th22 and Th17 cells in vitro
The chemotaxis assay was observed using light microscope with magnification of ×200. Briefly, the 8-µm pore polycarbonate filters in 24-well Transwell chambers (Corning Incorporated, Corning, NY, USA) were used. Transwell membranes were coated with fibronectin (5 µg/ml; Chemicon International; EMD Millipore, Billerica, MA, USA) for 30 min at 37°C. Purified CD4+ T cells from blood (2×105) were isolated using an EasySep™ Direct Human CD4+ T Cell Isolation kit (StemCell Technologies Inc., Vancouver, BC, Canada), then added into the top chamber and resuspended in RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) with 0.5% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.) (final volume, 100 µl). MA supernatants were placed in the bottom chamber in a volume of 600 µl and the chambers were incubated at 37°C in an atmosphere containing 5% CO2 for 3 h. Finally, all the cells that migrated into the bottom chamber were harvested, intracellularly stained for IL-22 or IL-17 and analyzed using flow cytometry as aforementioned. The chemotaxis index for Th22 and Th17 cells was calculated individually as follows: (Number of cells that migrated in response to MA or supernatants)/(number of cells that migrated in response to medium alone). To investigate whether CCL20, CCL22 and CCL27 contributed to Th22 or Th17 cell migration, inhibition experiments were performed by mixing the MA or supernatants with 100 ng/ml anti-CCL20 mAb (cat. no. 500-M28), anti-CCL22 mAb (cat. no. MAB336), anti-CCL27 mAb (cat. no. MAB376) or mouse immunoglobulin G irrelevant isotype control (cat. no. MAB0032) (R&D systems, Inc., Minneapolis, MN, USA), the anti-body was diluted for 1:100, and incubated at 4°C for 20 min.
Survival analysis for MA patients
To estimate the survival time of MA patients with different Th22 or Th17 cell, the patients were divided into cell high group or cell low group based on the median value of cell. The median value of Th17 cells and Th22 cells was 2.75, 3.02%, respectively, and patient had the median value was considered in high group in this study.
Statistical analysis
Data are expressed as the mean ± standard deviation. Comparisons of Th22, Th17, CCL20, CCL22, CCL27, IL-17 and IL-22 levels in MA, corresponding blood and peripheral blood from healthy controls were determined using a one-way analysis of variance (ANOVA). Newman-Keuls multiple comparison test (q test) was used to determine the difference between two groups when the one-way ANOVA analysis indicated a significant difference. Comparsion of Th22, Th17, CCL20, CCL22, CCL27, IL-17 and IL-22 levels in MA and corresponding blood was determined using Student's t-test. The Pearson or Spearman correlation test was used for correlation analysis, depending on the data distribution. Overall survival was estimated using the Kaplan-Meier method and compared using the log-rank test. Analysis was performed using SPSS version 16.0 statistical software (SPSS, Inc., Chicago, IL, USA) and P<0.05 was considered to indicate a statistically significant difference.
Results
Th22, Th17 and Th1 cells are increased in MA
It is reported that Th22 cells are associated with Th17 (16) and Th1 cells (17); therefore, in the present study, the distribution of Th22, Th17 and Th1 cells were studied in patients with MA, and healthy controls. Representative flow cytometry scatter plots of Th22, Th17 and Th1 cells in MA, and healthy controls are presented in Fig. 1A. The proportions of Th22 cells exhibited significantly increased values in MA in comparison with the corresponding peripheral blood and healthy controls. In addition, Th17 and Th1 cells in MA were significantly increased compared with the corresponding peripheral blood and healthy controls (Fig. 1B). The proportions of peritoneal Th22 cells were positively associated with Th17 or Th1 cells, and Th17 cells were positively associated with Th1 cells in MA (all P<0.01; Fig. 1C).
Th22 and Th17 cells exhibit similar phenotypic characteristics in MA and peripheral blood samples
The flow cytometry scatter plots of the expression levels of CCR4, CCR6 and CCR10 on Th22 cells in MA, and the corresponding peripheral blood are presented in Fig. 2A. The phenotypic analysis revealed that Th22 cells expressed high concentrations of CCR4, CCR6 and CCR10 in MA compared with the corresponding peripheral blood (Fig. 2B). The expression levels of CCR4 and CCR6 in Th17 cells from MA, and the peripheral blood are presented in Fig. 3A. The results revealed that Th17 cells expressed high concentrations of CCR4 (Fig. 3B) and CCR6 (Fig. 3C) in MA compared with the corresponding peripheral blood.
Chemokines CCL20, CCL22 and CCL27 are increased in MA, and may be associated with the migration of Th17 and Th22 cells
The concentrations of chemokines CCL20, CCL22 and CCL27 in MA were significantly increased, compared with that in the corresponding peripheral blood, determined using ELISA (Fig. 4A-C), which are receptors for CCR4, CCR6, and CCR10, respectively. On the basis of these results, it was hypothesized that peripheral Th22 and Th17 cells may infiltrate into the peritoneal cavity in response to these chemokines. In the chemotaxis assays, it was revealed that the anti-CCL20, anti-CCL22 and anti-CCL27 mAbs significantly suppressed Th22 cell migration; whereas, the anti-CCL20 mAb markedly inhibited Th17 cell migration (Fig. 4D and E).
Increased concentrations of IL-22 and IL-17 in MA
The concentration of IL-22 was significantly increased in MA, compared with the corresponding peripheral blood and healthy controls, determined using ELISA (Fig. 5A). Additionally, the concentration of IL-17 was significantly increased in MA compared with that in the corresponding peripheral blood and healthy controls (Fig. 5B). There was a positive association identified between Th22 cells and the concentrations of IL-22 in MA (Fig. 5C). Furthermore, there was a positive association revealed between IL-22 and Th17 cells in MA (P=0.001; Fig. 5D). However, no association was identified between IL-22 and Th22 or Th17 cells in either the peripheral blood or healthy controls (all P>0.05; data not shown).
Increased number of Th17 cells indicates an increased overall survival time
On the basis of the median value of Th17 cells (2.75%), the 26 patients with MA were divided into either the Th17 cell high group (n=13) and Th17 cell low group (n=13). As presented in Fig. 6A, patients with in the high Th17 cell group exhibited an increased overall survival time, compared with that in the low Th17 cell group [hazard ratio (HR), 1.75; 95% confidence interval (CI), 1.29–2.21; P=0.012]. The median Th22 cells value was 3.02%; however, no significant difference was identified between the Th22 cell high group (n=13) and the Th22 cells low group (n=13) (HR, 0.48; 95% CI, 0.19–1.23; P=0.127) (Fig. 6B).
Discussion
To the best of our knowledge, the present study was the first to investigate the distribution, the phenotypic characteristics and the recruitment of Th22, and Th17 cells in patients with HCC and MA. The results of the present study demonstrated that the proportion of Th22 and Th17 cells were significantly increased in MA compared with the corresponding peripheral blood and healthy controls, indicating the involvement of Th22 and Th17 cells in the development of MA. In addition, there was a positive association identified between IL-22 and Th22 cells, Th17 cells in MA, suggesting that Th22 and Th17 cells may have a similar effect through the regulation of IL-22. However, the association between Th22 and Th17 cells requires additional study.
IL-22 and IL-17 may induce Th cell differentiation, and IL-22 may be more widely involved in IFN-γ-producing CD4+ T cell (Th1 cell)-mediated immune responses (15). Furthermore, it is IL-22, not IL-17, that may induce wound-healing responses, and tissue-repair protecting from tuberculosis (13,18). IL-22 is predominantly secreted by Th22, Th17 and Th1 cells (19). Additionally, of the IL-22-producing T cells, the proportion of Th22 cells accounts for between 37 and 63%, Th17 cells accounts for between 10 and 18%, and Th1 cells accounts for ~35% (20). It has been demonstrated that IL-6 promotes the expression of IL-22, and is required for IL-17 induction from naïve T cells (21,22); this may explain the positive association between the concentration of peritoneal IL-22 and the number of peritoneal Th22 cells, and the positive association between IL-22 and Th17 cells identified in the present study. In addition, the results of the present study indicated that proinflammatory cytokines in MA may promote the secretion of IL-22.
Some mechanism has been identified to increase the number of Th22 and Th17 cells. In addition to the local generation and differentiation, the reason for the increase in peritoneal Th22 and Th17 cells may have been in response to recruitment from the peripheral blood (13). A previous study demonstrated that the migration of lymphocytes is regulated by the chemokine/chemokine receptor axis (23). CCL20/CCR6, CCL22/CCR4 and CCL27/CCR10 chemotaxis axes have been identified to serve functions in the recruitment of lymphocytes to local tissues (24). Previously studies have demonstrated that the recruitment of Th9 cells into malignant pleural effusion may be induced by pleural CCL20 (25), and that CCL20 and CCL22 were responsible for the recruitment of IL-27-producing CD4+T cells to tuberculous pleural effusion (13,18). However, the function of the chemokine/chemokine receptor interaction in the recruitment of peripheral Th22 cells and Th17 cells into MA remain unknown.
In the phenotypic analysis of the present study, the results revealed that Th22 cells expressed increased concentrations of CCR4, CCR6 and CCR10, whereas Th17 cells expressed increased concentrations of CCR4, and CCR6 in MA compared with the corresponding peripheral blood. In addition, the concentrations of CCL20, CCL22 and CCL27 in MA were markedly increased, compared with those in the plasma from corresponding peripheral blood. These results indicated that the axes of CCL20/CCR6, CCL22/CCR4 and CCL27/CCR10 may be involved in the recruitment of Th22, and Th17 cells to the peritoneal cavity from the peripheral blood. Using chemotaxis assays, it was observed that, by inhibiting the CCL20/CCR6, CCL22/CCR4 and CCL27/CCR10 axes, the migration rate of Th22 cells was significantly suppressed, and inhibition of CCL20/CCR6 prevented Th17 cell migration, indicating that these chemotaxis axes may be responsible for the recruitment of peripheral Th22 and Th17 cells into MA.
Previously, it was reported that high expression of IL-17 was associated with poor prognosis, whereas high Th17 cell frequencies were associated with improved prognosis (26). Th17 cells are a subpopulation of IL-17+ cells and exhibit a distinct association with prognosis compared with total IL-17 (27). Th17 cells serve an antitumor function in antitumor immunity and promote the immune response of tumor-specific cytotoxic T cells in the pulmonary melanoma model (28). Accumulation of Th17 cells in malignant pleural effusion indicates the improvement of patient survival (12) and a decrease in Th17 cells in ascites from patients with ovarian cancer indicated a poor prognosis (29). Similar to these aforementioned studies, the present study demonstrated that the accumulation of Th17 cells in MA indicated the improvement of patient survival.
Th22 cells accumulate in a number of different types of tumor, compared with healthy tissues, and the patient outcome may differ from one tumor type to another (30). Previous studies have demonstrated that increased Th22 cells were associated with poor prognosis of patients with pancreatic cancer (31), multiple myeloma (32) and colorectal cancer (33); however, Th22 cells are protective in colorectal cancer (34). In the present study, no significant different was identified in the survival time between the Th22 cell high and Th22 cell low groups of patients. Therefore, it was hypothesized that the different prognostic functions of Th22 and Th17 cells may be due to the cytokines secreted by the different cell types or the tumor type, including HCC or ovarian cancer at stage III or IV; however, the underlying molecular mechanisms require additional study.
The results of the present study demonstrated that Th22 and Th17 cells may serve functions in the pathogenesis of MA, and accumulation of Th22 cells and Th17 cells in MA may be due to locally increased proinflammatory cytokines and chemokines. Furthermore, increased numbers of Th17 cells in MA indicates an improved survival time for patients with HCC. However, due to the limited number of samples included, a study with larger umber samples is warranted, and an in vivo animal experiment to further validate these results is needed.
Acknowledgements
Not applicable.
Funding
The present study was partially supported by the Guangxi Natural Science Foundation (grant no. 2014GXNSFAA118203). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Authors' contributions
SYQ and HXJ designed the study, interpreted the experimental results and modified the manuscript. XWY, RL and WSL collected the samples and performed the experiment. XWY and SHT performed statistical analysis. All authors have read and approved the manuscript.
Ethics approval and consent to participate
The present study was approved by the Ethics Committee of the First Affiliated Hospital of Guangxi Medical University for human studies and all patients provided written informed consent.
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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