Hereditary breast and ovarian cancer susceptibility genes (Review)
- Authors:
- Published online on: June 19, 2013 https://doi.org/10.3892/or.2013.2541
- Pages: 1019-1029
Abstract
1. Introduction
Breast cancer and ovarian cancer develop through multiple molecular pathways guided by genetic and epigenetic clonal selections. Both cancers have been recognized as a heterogeneous disease with regard to clinical and biological properties. The majority of cancer cases are considered sporadic-appearing tumors in nature because there is no obvious family history, but a number of families with a known genetic cause or inherited predisposition to cancer have been identified (1). Individuals who carry an inherited genetic mutation and epigenetic aberrations in the tumor suppressor genes have an increased lifetime risk of developing cancer. Germline mutations in cancer susceptibility genes cause cancer if the wild-type allele is lost or inactivated. Breast and ovarian cancers (5–10%) may be hereditary and occur in cancer prone syndromes (2–7). Patients predisposed to breast and ovarian cancers are known as the hereditary breast and ovarian cancer (HBOC) syndrome.
HBOC syndrome is an autosomal dominantly inherited disease characterized by a young age of onset, more than one synchronous or metachronous tumor, and a family history of first and second degree relatives with similar cancers (8). Mainly HBOC syndrome results from germline mutations in breast cancer genes BRCA1 or BRCA2. Other genes or low penetrance alleles might be associated with the HBOC phenotype (9). There is an increasing understanding that the interrelationship between BRCA gene cluster and Fanconi anemia (FA), mismatch repair (MMR) and DNA repair gene status plays a key role in the pathogenesis of cancer predisposition syndromes. We reviewed the HBOC syndrome and current knowledge of inherited susceptibility genes.
2. Materials and methods
A computerized literature search was performed to identify relevant studies reported in the English language. We searched MEDLINE electronic databases (http://www.ncbi.nlm.nih.gov/sites/entrez) published between January 1994 and October 2012, combining the keywords ‘hereditary breast and ovarian cancer’, ‘pathogenesis’, ‘BRCA’ and ‘DNA repair’. Various combinations of the terms were used, depending on the database searched. Each gene was also linked to NCBI Entrez Gene pages (http://www.ncbi.nlm.nih.gov/sites/entrez). Additionally, references in each article were searched to identify potentially missed studies.
3. Current understanding of HBOC susceptibility genes
BRCA1 and BRCA2
The breast cancer-associated genes BRCA1 on chromosome 17q and BRCA2 on chromosome 13q are the most well-known breast cancer susceptibility genes (4,5). The HBOC syndrome is linked to the BRCA1 gene and, to a lesser extent, to the BRCA2 gene. Germline mutations in these genes account for 2–5% (up to 10%) of all breast cancers and all ovarian cancers. Mutations were present with a frequency of more than 10% in the high risk populations, including patients with a family history of breast or first-degree ovarian cancer, those with bilateral breast cancer, multiple organ cancer including younger breast cancer patients (aged <35 years). Mutations in the BRCA1 and BRCA2 genes are responsible for ~60% (up to 85%) of HBOC. Women carrying a BRCA1 or BRCA2 genetic mutation have 60–80% and 20–40% lifetime risk of developing breast cancer and ovarian cancer, respectively. More modest increases in risk for other cancers have been noted: additional sites included stomach, pancreas, prostate and colon. The cancer risk ranged from 20 to 60%, with the greatest increases in cancer risk in stomach and pancreas. BRCA mutations were also associated with increased risks for leukemia and lymphoma (10).
A recent study showed that breast cancer patients belonging to a population with a high probability of being BRCA1 carriers showed a better prognosis compared with those with sporadic breast cancer (11). Furthermore, BRCA1 and BRCA2-related invasive epithelial ovarian cancers have a better 5-year overall survival compared with sporadic ovarian cancers (12). The 5-year overall survival was better in BRCA2 carriers compared to BRCA1 carriers (36% for non-carriers, 44% for BRCA1 carrier and 52% for BRCA2 carriers).
The mutations include partial or complete gene deletions, duplications, large insertions, splice alteration, frameshifts as well as missense and nonsense mutations. Deletions or insertions usually lead to abnormal structure and function. Germline mutations are usually pathogenic point mutations, and are scattered throughout their coding regions. The potential hot-spot mutations within BRCA 1 and BRCA2 are uncommon. The previously described mutations were identified in the Breast Cancer Information Core website (BIC, http://research.nhgri.nih.gov/bic/). More than 3500 mutations have been reported throughout both genes. Data from subjects with a variety of ethnic backgrounds altered the overall odds of BRCA mutation carrier status. The spectrum of mutations is different depending on the race. The mutations are detected in 10–12% of Ashkenazi Jewish women diagnosed with breast cancer. Ashkenazi Jewish subjects are observed at increased frequency compared to other Caucasian, because this population harbors ancient BRCA1 and BRCA2 mutant alleles. Mutation analysis revealed the c. 5266dup (until recently referred in the literature as 5382insC), c. 68_69del (185delAG) and 4153delA mutations in BRCA1 and c. 5946del (6174delT) mutation in BRCA2 (13). BRCA gene founder mutations such as BRCA1c. 5266dup mutation, BRCA2999del5 mutation and BRCA1delexon17 have also been described in other populations, including the Slavic, Finnish, Icelandic and German populations, respectively.
The tumor suppressor BRCA1 and BRCA2 genes are essential components of the double-strand break (DSB) repair by homologous recombination (HR) system. Targeting tumor suppressor loss-of-function is possible based on the concept of synthetic lethality. Thus, the synthetically lethal effect might be observed in tumors defective in BRCA1 or BRCA2 that are required for efficient HR, indicating that ovarian cancer patients carrying germ-line mutations had improved rates of progression-free and overall survival (12). Chromosomal rearrangements might be formed as a consequence of these error-prone DSB repairs and lead to the development of genomic instability. Large genomic rearrangements have been identified in HBOC families and account for 8–15% of deleterious BRCA mutations, but these rearrangements may escape detection (14). The issue is that BRCA genetic testing done through sequencing will not capture large rearrangements in these genes.
BRCA1 functions as a tumor suppressor gene, but paradoxically, BRCA1 knockout mice are embryonically lethal in homozygous state. Lack of BRCA1 is thought to result in cellular lethality, suggesting that BRCA1 regulates stem/progenitor cell proliferation and differentiation. For cell differentiation, BRCA1 regulates apicobasal polarity, together with several genes such as RHAMM (hyaluronan-mediated motility receptor), AURKA (aurora kinase A) and TPX2 (microtubule-associated, homolog). Intracellular RHAMM associates with BRCA1 and BARD1 (BRCA1 associated RING domain 1). The complex attenuates the mitotic-spindle-promoting activity of RHAMM that might contribute to tumor progression. BRCA1 also binds and regulates AURKA, a cell cycle-regulated kinase that appears to be strongly involved in centrosome regulation. Genetic variants in the AURKA gene may contribute to breast cancer development (5). BRCA1 further accumulates TPX2 and is required for mitotic spindle-pole assembly. BRCA-associated nuclear core complex proteins are required for the functional integrity of the pathway of not only DNA damage response and repair, but also cell differentiation
From a clinical point of view, the triple-negative breast cancer characterized by the absence of estrogen receptor (ER), progesterone receptor (PgR), and HER2 (also known as ERBB2) accounts for ~15% of breast cancers and is diagnosed more frequently in younger women (15). Compared with the ER/PR-positive tumors, triple-negative breast cancershowed a greater risk for recurrence and shortened survival. Triple-negative breast cancer was frequently associated with mutations in BRCA genes: the incidence was 12.5–20% (16). Women with an early age-of-onset triple-negative breast cancer are more likely to be associated with deleterious mutations in BRCA1 and BRCA2 genes (17). Even in non-BRCA gene mutations, a subset of triple-negative tumors shares multiple clinicopathologic features and phenotype with BRCA-mutated breast cancers. They harbor dysfunctional DNA repair mechanisms, but the nature of this link remains opaque. A subset of HBOC syndrome also contain mutations in the TP53 gene, and the TP53 loss-of-function tumors have a low frequency of HER2 expression (18).
The predominant histologic type of ovarian cancers associated with the HBOC syndrome was high-grade serous carcinomas of the ovary. There were no significant differences in ovarian cancer morphology between BRCA1 and BRCA2 carriers (19). Ovarian cancer patients with BRCA mutations were associated with an increased chemosensitivity and improved overall survival, but some investigators failed to confirm improved survival.
Modifying hereditary breast and ovarian cancer risks
Mutations in BRCA1 and BRCA2 do not account for all cases of HBOC, implicating that the remaining cases can be attributed to the involvement of other susceptibility genes. Other genes, including Fanconi anemia (FA) cluster (FANCD2, FANCA and FANCC), MMR cluster (MLH1, MSH2, PMS1, PMS2 and MSH6), DNA checkpoint cluster (ATM, ATR and CHK1/2), and tumor suppressor cluster (TP53, SKT11 and PTEN) have been associated with increased risk of breast and ovarian cancer as part of other cancer syndromes. The contribution of mutations in other genes to the burden of breast or ovarian cancer is indicated in Table I.
Poly(ADP-ribose) polymerase (PARP)
Poly(ADP-ribose) polymerase (PARP) is an enzyme involved in the recovery of cells from DNA damage and the regulation of the molecular events such as BER, a key pathway in the repair of DNA single-strand breaks (SSB). The inhibition of PARP leads to the induction of synthetic lethality and cell death by targeting HR-mediated DNA repair deficient tumors (20). Tumors that lack functional BRCA1, BRCA2, or TP53 are hypersensitive to inhibition of PARP. Several proteins involved in HR on sensitivity to PARP inhibition may include BRCA cluster (RAD51C, RAD51D and RAD54), FA cluster (FANCD2, FANCA and FANCC), Cdk cluster, nucleotide excision repair (NER) cluster (RPA1 and NBN), DNA repair checkpoint cluster (ATR, ATM, CHK1 and CHK2) and TP53 cluster. Therefore, therapeutic approach using PARP inhibitors may be feasible for BRCA dysregulated tumors and appear promising in a variety of cancer types, including breast and ovarian cancers. The presence of these germline mutations and epimutations types might be a hallmark of BRCAness and a potential biomarker for sensitivity to PARP inhibition.
Recently, Kaye et al(21) reported the results of a phase II study comparing the efficacy and safety of olaparib, a potent oral PARP inhibitor, in patients with germ-line BRCA mutations and recurrent ovarian cancer. Unfortunately, there was no remarkable differences in PFS between the olaparib and pegylated liposomal doxorubicin groups. Another clinical study demonstrated that olaparib applied as maintenance treatment prolonged PFS, but not OS, in patients with advanced and platinum-sensitive, recurrent, high-grade serous ovarian cancer (22).
BER and NER
There are two types of DNA repair proteins: the nucleotide excision repair (NER) pathway (ERCC1, excision repair cross-complementing rodent repair deficiency, complementation group 1) and the base excision repair (BER) pathway (XRCC1, X-ray repair complementing defective repair in Chinese hamster cells 1) (23). The NER pathway is responsible for the removal of bulky DNA lesions. NER is a defense system against various types of DNA damage and necessary for maintaining genomic stability. RPA1, replication protein A1, is a single-stranded DNA binding protein and participates in the recruitment of the two structure-specific DNA endonucleases, XPG (xeroderma pigmentosum, also known as ERCC5) and XPF (xeroderma pigmentosum, complementation group F)-ERCC1 complex, which makes the 5′ incision in NER. The XPF-ERCC1 complex is essential for cutting the damaged DNA strand and the DNA repair by the NER pathway. NBN (nibrin, also known as NBS1) was involved in DNA DSB repair and DNA damage-induced checkpoint activation as a component of the MRE11-RAD50-NBS1 complex. Mutations in NBN is thought to be associated with breast-cancer risk.
Furthermore, XRCC1 is involved in the repair of DNA SSB and oxidative damage (23). This protein interacts with DNA ligase III, polymerase β and PARP1 participating in the BER pathway. Reduced XRCC1 expression may confer chemoradiation and leads to improved patient survival.
4. Epigenetic silencing
Between 50–80% of HBOC syndrome can be explained by defective germline mutations in BRCA1 and BRCA2 as well as, to a lesser degree, other genes described above, but for the remaining families the factors driving susceptibility remain unknown (24). Approximately one third of the HBOC families do not have evidence of the germline mutations in BRCA1 and BRCA2. The loss of BRCA function might be due to either germline/somatic mutation or epigenetic silencing. Since little is known about the contribution of epimutations to the remaining BRCA1/2 mutation-negative cases, epigenetic silencing has been explored in HBOC syndrome. The activities of tumor suppressor genes and cancer susceptibility genes could be influenced by genetic and epigenetic alterations. Decreased expression of cancer susceptibility genes has been observed in sporadic breast and ovarian cancer where it is often associated with the aberrant epimutations or hypermethylation of the BRCA1 and BRCA2 genes. The loss of BRCA1 function due to somatic hypermethylation explained ~10% of sporadic breast cancer cases. A subset of the sporadic tumor patients demonstrated hypermethylation of BRCA2 and their interacting protein including HP1γ (heterochromatin protein 1gamma), RAD51C, ATM and PALB2 (25). Based on BRCA1 deletion, TP53 mutations, ER- and PgR-negative status, young age at diagnosis and high grade tumor, phenotypic features of sporadic breast cancers resemble BRCA1 mutated cancers termed ‘BRCAness’. Phenotypic similarities were most closely observed in BRCAness, epigenetic silencing and deletion of the BRCA1 and BRCA2 genes.
5. Lynch syndrome
Many genes have been implicated in the DNA damage response pathways where the BRCA1 and BRCA2 genes are involved. Genetic susceptibility to cancer is attributed to deleterious germline mutations in the DNA mismatch repair (MMR) genes. Another important non-BRCA1/BRCA2 hereditary condition is hereditary non-polyposis colorectal cancer (HNPCC) syndrome, also known as ‘Lynch syndrome’. Two manifestations of hereditary ovarian cancer are currently recognized: the HBOC syndrome and the HNPCC syndrome. Lynch syndrome has been defined clinically and genetically and is an autosomal-dominant cancer predisposition syndrome that increases the risk for several forms of malignancy, including colorectal (lifetime cancer risk, 70–80%), endometrial (50–60%), stomach cancer (13–19%), ovarian cancer (9–14%), small intestine, liver and biliary tract, brain, as well as transitional cell carcinoma of the ureters and renal pelvis. Mutations in four MMR genes [MLH1 (mutL homolog 1), MSH2 (mutS homolog 2), MSH6 (mutS homolog 6) and PMS2 (postmeiotic segregation increased 2)] are associated with Lynch syndrome and account for another 10% of hereditary ovarian cancer (26). The MMR genes encode proteins involved in the same pathway of DNA mismatch repair. These genetic defects in the DNA MMR system result in DNA replication errors, including base substitutions and insertion-deletion loops, known as microsatellite instability (MSI). MutS α complex (composed of MSH2 and MSH6) or MutS β complex (MSH2–MSH3) recognizes single-base mismatches and small insertion-deletion loops, binds to mismatched DNA, and recruits the MutL α complex (MLH1–PMS2), which leads to strand discrimination and removal of the errors and coordinates the remaining steps in MMR (27–29).
Both HNPCC and HBOC associated ovarian cancer develop along distinct genetic pathways (30). Although some deviating reports exist, breast cancer incidence has been found to be elevated in Lynch syndrome patients (31). Furthermore, germline mutations associated with Lynch syndrome has been described in 2–3% of patients diagnosed with endometrial cancer. Among Lynch syndrome-related cancers, endometrial cancer is riskier than colorectal cancer in terms of estimated lifetime cumulative risk (32). The overall 5-year survival rate for endometrial (88 vs. 82%) or ovarian cancer (64 vs. 58%) was not significantly different between patients with endometrial or ovarian cancer that are associated with Lynch syndrome and the controls with sporadic cases (33,34). Although immunohistochemical analysis of tumor tissue proved to be a good pre-screening test before proceeding to germline mutation analysis, the discordant results are sometimes observed between immunohistochemistry and replication error phenotyping. Notwithstanding this limitation, immunohistochemistry may be helpful in the evaluation of women with a likely diagnosis of Lynch syndrome. Immunohistochemistry for DNA MMR showed loss of proteins in 9% of women with synchronous endometrial and ovarian cancer (35).
As shown in Fig. 1, BRCA1 forms a multi-subunit protein complex, which includes DNA damage repair proteins, including FA and MMR proteins. Recent studies showed a functional interaction between FANCJ and the MMR complex MutL α, which is essential for establishment of DNA interstrand cross-links (36). FANCD2 is required for binding between MSH2 and MLH1, which are involved in the mono-ubiquitination of FANCD2, leading to recruitment of ATR and then activation of CHK1 and TP53. Furthermore, MutS α and MutL α complex have been shown to be required for the recruitment of ATR to DNA damage lesion. Taken together, these results support that there might be a functional overlap between the MMR and FA-BRCA pathways.
6. Prevention
Attention has been paid to the role of modifiable risk factors like reproductive histories and exogenous hormones. Potential modifying factors include age of menarche, parity, breastfeeding and oophorectomy. Oral contraceptives (OCs) have a significant protective effect on the risk of ovarian cancer by ~50% in the general population (28). OCs reduce the risk of ovarian cancer also in BRCA1/BRCA2 mutation carriers. The effect of parity may be different in BRCA1 and BRCA2 carriers. Parity protects against breast cancer in BRCA1 mutation carriers. Multiparity may be associated with an increase in risk in BRCA2 carriers. However, the effect of multiparity on ovarian cancer risk for BRCA2 mutation carriers has only been investigated in a small number of studies. Therefore, the association is controversial. Women who took OCs before the age of 30 years and long-term user of 5 or more years have been associated with a slight increase in risk of breast cancer among BRCA1 mutation carriers (29). Furthermore, prolonged hormone replacement therapy (HRT) use is an established risk factor of breast cancer. HRT also affects ovarian cancer risk. Among a subgroup of patients who had a familial history of breast cancer, tamoxifen and raloxifene, selective estrogen receptor modulators, reduced breast cancer risk. Tamoxifen and raloxifene reduced the risk of invasive breast cancer: compared with placebo, raloxifene reduced breast cancer risk by 38%, tamoxifen showed a 50% reduction (37). Additionally, use of PARP inhibitors is a potential synthetic lethal therapeutic strategy and may be considered as targeted chemoprevention in patients with specific DNA-repair defects. Other agents under preclinical and clinical investigation include cyclooxygenase-2 inhibitors, aromatase inhibitors, tyrosine kinase inhibitors, and difluoromethylornithine (a polyamine inhibitor) (38,39). Future efficacy studies are expected.
Prophylactic surgeries are appropriate treatment options for BRCA mutation-associated cancer (4). Prophylactic bilateral mastectomy reduces the risk of breast cancer in BRCA mutation carriers by 90% at any age. Prophylactic bilateral salpingo-oophorectomy (BSO) lowers the ovarian cancer risk by 80%. BSO performed before age 50 years also exhibits a 50% reduction in subsequent breast cancer risk. In conclusion, prophylactic surgeries lead to a reduction in breast and ovarian cancer-specific mortality. Knowledge of these risk factors and prevention strategies will have a great impact on the management of hereditary breast and ovarian cancers.
7. Screening
Since identification of the mutation screening is currently labor intensive and expensive, the screening should be directed to asymptomatic individuals only if they belong to high-risk families. Various safe and effective screening protocols have been recommended for early cancer detection and reduction of cancer risk in clinical practice. Women with HBOC syndrome often utilize the latest medical advances in increased surveillance, prevention, early detection, chemoprevention and optimal treatment. Among BRCA1 and BRCA2 mutation carriers, use of screening mammography alone led to increased early detection rates of non-palpable breast cancer, but the rate of interval cancers was high (7,40). Therefore, the effectiveness of mammography alone (sensitivity 40%) is questionable for screening high-risk women. In the high risk group of women, magnetic resonance imaging (unenhanced MR imaging with combined diffusion-weighted and T2-weighted images, sensitivity 50%) surveillance largely out performed mammography. Furthermore, dynamic contrast-enhanced MRI exhibited highest sensitivity (86%) (41). MRI is a better screening method and will detect the majority of breast cancers at an early stage. The addition of MRI to screening mammography increased sensitivity (42), supporting the benefits of breast MRI examination annually (the sensitivity was 80%, the false positive rate was 10%) in BRCA mutation carriers (43). Alternating MRI and mammography screening at 6-month intervals might be a clinically effective approach. The HBOC carriers have two options to reduce their risk of ovarian cancer: periodic screening and risk-reducing surgeries (44). Periodic screening consists of annual or semi-annual pelvic examination with the longitudinal CA125 blood test plus concurrent transvaginal ultrasound. Unfortunately, to date, this screening regimen is ineffective for early detection of ovarian cancer in high-risk women. Risk-reducing salpingo-oophorectomy represents a potentially valuable intervention and is the only way for many women at high-risk by age 40 years, or on completion of childbearing. Preventive surgery can reduce ovarian cancer risk by 80–90% and breast cancer risk by 50–60% in BRCA mutation carriers.
8. Discussion
HBOC syndrome is the inherited tendency to develop breast, ovarian and other cancers and believed to be transmitted by mutations in the specific genes. Clinical characteristics, including the type of tumor and age at occurrence as well as family history, predict the prevalence of BRCA germline mutations. A number of clinicians usually take into account the age of the youngest breast cancer patient and the number of ovarian cancer cases in a family as well as pathological diagnosis. Up to 80% of the HBOC cases are due to mutations in BRCA1 or BRCA2 genes. Both BRCA1 and BRCA2 mutations are scattered throughout the whole coding exons.
To maintain and restore the genomic integrity, normal cells possess DNA repair mechanisms. The structural modifications, such as DNA base damage, DNA strand break, inter- and intra-strand crosslinks and DNA-protein crosslinks, are involved in mutation and cancer. A variety of intelligent mechanisms can activate DNA repair pathways and cell cycle checkpoints and recognize and repair SSB or DSB by the master sensors and regulators of DNA damage response such as BRCA1 and BRCA2. BRCA1 and BRCA2 genes were recruited to the sites of DNA damage. BRCA1 associates with several proteins and is an integral member of the repair of DNA damage by functional HR, NER and possibly NHEJ. BRCA1 activated by cdk1 physically interacts with MutL α, through the interaction of the FA-BRCA pathway (45). BRCA2, also known as FANCD1, has a more specific role in DNA repair and is directly involved in the mechanism of HR, regulating the activity of RAD51, a gene implicated in the HR pathway as well as interacting with PALB2, a gene implicated in the HR repair and checkpoint functions. Interestingly, the nuclear function of BRCA proteins is tightly regulated by the FA-BRCA, MMR, BER and NER pathway. However, the exact mechanisms of the BRCA-associated interaction and accumulation of other DNA repair proteins are not comprehensively known. Therefore, germline mutations in other susceptibility genes, such as FA genes, MMR genes and DNA repair genes, might be the predisposing factors in HBOC cases. These predisposing genes encode for upstream and downstream regulators of BRCA gene products and also may be associated with the BRCA core complex, including mutations in FANCD1, FANCD2, FANCJ, FANCP, TP53, PTEN, STK11, CDH1, CHK2, ATM, ATR, MSH1, M SH2, MLH1 and PMS2 genes. Families affected by other syndromes, such as Lynch syndrome (mutations in MMR genes), Fanconi anemia, Cowden syndrome (mutations in PTEN), Li-Fraumeni syndrome (mutations in TP53), xeroderma pigmentosum and ataxia-telangiectasia, exhibit additional types of cancers outside the previously defined HBOC cancer spectrum.
In conclusion, genetic or epigenetic loss-of-function mutations of genes that are known to be involved in the repair of DNA damage might lead to increased risk of developing a broad spectrum of breast and ovarian cancers.
Acknowledgements
Th present review was supported by grant-in-aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan to the Department of Obstetrics and Gynecology, Nara Medical University (to H.K.).
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