Evaluation of the effect of methionine and glucosamine on adjuvant arthritis in rats

Yamagishi,Yoshie; Igarashi,Mamoru; Suzuki,Atsuko; Suguro,Shiori; Hirano,Shin-Ichi; Nagaoka,Isao

doi:10.3892/etm.2012.668

Evaluation of the effect of methionine and glucosamine on adjuvant arthritis in rats

Authors:
- Yoshie Yamagishi
- Mamoru Igarashi
- Atsuko Suzuki
- Shiori Suguro
- Shin-Ichi Hirano
- Isao Nagaoka
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Affiliations: Protein Chemical Co. Ltd., Tokyo 100‑0011, Japan, Department of Host Defense and Biochemical Research, Juntendo University, Graduate School of Medicine, Tokyo 113‑8421, Japan , Mercian Cleantec Corporation, Fujisawa, Kanagawa 250‑0057, Japan
Published online on: August 16, 2012 https://doi.org/10.3892/etm.2012.668
Pages: 640-644

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Abstract

In the present study, we evaluated the effects of individual administration of methionine or glucosamine (GlcN) and compared with the combined administration of methionine and GlcN on the adjuvant arthritis model of rheumatoid arthritis in rats. Adjuvant arthritis was induced in female Lewis rats by injecting Freund's complete adjuvant (FCA) into the right hind paws, and methionine (200 mg/kg body weight/day) and/or GlcN (400 mg/kg/day) were orally administered for 21 days. The progression of the adjuvant arthritis was clinically evaluated for characteristic signs and symptoms by employing an arthritis score. The administration of methionine combined with GlcN suppressed the swelling of FCA-uninjected left hind paws and the arthritis score. Additionally, histopathological examination revealed that the combined administration of methionine and GlcN markedly suppressed synovial hyperplasia and the destruction of the cartilage surface and articular meniscus of the knee joints of FCA‑injected right hind paws. Furthermore, combined methionine and GlcN administration suppressed the increase in the levels of nitric oxide, prostaglandin E2 and hyaluronic acid in the plasma of rats with adjuvant arthritis. By contrast, individual administration of methionine or GlcN suppressed arthritis only slightly. These observations suggest that the combined administration of methionine and GlcN is more effective compared with individual administrations of methionine or GlcN in suppressing the progression of adjuvant arthritis (identified as swelling of joints and arthritis score), possibly by synergistically inhibiting synovial inflammation (identified as synovial hyperplasia and the destruction of the cartilage surface and articular meniscus) and the production of inflammatory mediators.

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