Effect of surgical intervention on the expression of leukemia inhibitory factor and L‑selectin ligand in the endometrium of hydrosalpinx patients during the implantation window

Zhong,Yiping; Li,Jin; Wu,Haitao; Ying,Ying; Liu,Yafeng; Zhou,Canquan; Xu,Yanwen; Shen,Xiaoting; Qi,Quan

doi:10.3892/etm.2012.728

Effect of surgical intervention on the expression of leukemia inhibitory factor and L‑selectin ligand in the endometrium of hydrosalpinx patients during the implantation window

Authors:
- Yiping Zhong
- Jin Li
- Haitao Wu
- Ying Ying
- Yafeng Liu
- Canquan Zhou
- Yanwen Xu
- Xiaoting Shen
- Quan Qi
View Affiliations

Affiliations: Reproductive Medicine Center, The First Affiliated Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China, Guangdong No. 2 Provincial People's Hospital, Guangzhou, Guangdong 510000, P.R. China
Published online on: September 28, 2012 https://doi.org/10.3892/etm.2012.728
Pages: 1027-1031

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The aim of this study was to investigate the effect of surgical intervention on the expression of leukemia inhibitory factor (LIF) and L‑selectin ligand in the endometrium of patients with hydrosalpinx during the implantation window. A total of 60 patients with hydrosalpinx and 30 patients with tubal obstruction were recruited, and immunohistochemistry was performed to detect the expression of LIF and L‑selectin ligand in the endometrium of hydrosalpinx patients before and after surgery and in the endometrium of patients with tubal obstruction. The expression of LIF and L‑selectin ligand in the endometrium of hydrosalpinx patients before surgery was markedly lower than that of patients with tubal obstruction (P<0.05). Following surgery, the expression of LIF and L‑selectin ligand in the endometrium of hydrosalpinx patients was comparable to that of patients with tubal obstruction (P>0.05). In addition, there was a pronounced difference in the expression of LIF and L‑selectin ligand in the endometrium before and after surgery in patients with hydrosalpinx (P<0.05). Hydrosalpinx reduces the expression of LIF and L‑selectin ligand in the endometrium during the implantation window. LIF and L‑selectin ligand may be important factors influencing the endometrial receptivity of hydrosalpinx patients, and surgery is capable of improving the expression of LIF and L‑selectin ligand in the endometrium during the implantation window.

View Figures

View References

1	Lessey BA: Assessment of endometrial receptivity. Fertil Steril. 96:522–529. 2011. View Article : Google Scholar : PubMed/NCBI
2	Taylor E and Gomel V: The uterus and fertility. Fertil Steril. 89:1–16. 2008. View Article : Google Scholar
3	Mijatovic V, Veersema S, Emanuel MH, et al: Essure hysteroscopic tubal occlusion device for the treatment of hydrosalpinx prior to in vitro fertilization-embryo transfer in patients with a contraindication for laparoscopy. Fertil Steril. 93:1338–1342. 2010. View Article : Google Scholar
4	Seli E, Kayisli UA, Cakmak H, et al: Removal of hydrosalpinges increases endometrial leukaemia inhibitory factor (LIF) expression at the time of the implantation window. Hum Reprod. 20:3012–3017. 2005. View Article : Google Scholar : PubMed/NCBI
5	Paiva P, Menkhorst E, Salamonsen L and Dimitriadis E: Leukemia inhibitory factor and interleukin-11: critical regulators in the establishment of pregnancy. Cytokine Growth Factor Rev. 20:319–328. 2009. View Article : Google Scholar : PubMed/NCBI
6	Aghajanova L, Altmäe S, Bjuresten K, et al: Disturbances in the LIF pathway in the endometrium among women with unexplained infertility. Fertil Steril. 91:2602–2610. 2009. View Article : Google Scholar : PubMed/NCBI
7	Aghajanova L: Update on the role of leukemia inhibitory factor in assisted reproduction. Curr Opin Obstet Gynecol. 22:213–219. 2010. View Article : Google Scholar : PubMed/NCBI
8	Dimitriadis E, Nie G, Hannan NJ, et al: Local regulation of implantation at the human fetal-maternal interface. Int J Dev Biol. 54:313–322. 2010. View Article : Google Scholar : PubMed/NCBI
9	van der Gaast MH, Macklon NS, Beier-Hellwig K, et al: The feasibility of a less invasive method to assess endometrial maturation - comparison of simultaneously obtained uterine secretion and tissue biopsy. BJOG. 116:304–312. 2009.
10	Mikolajczyk M, Wirstlein P and Skrzypczak J: The impact of leukemia inhibitory factor in uterine flushing on the reproductive potential of infertile women-a prospective study. Am J Reprod Immunol. 58:65–74. 2007. View Article : Google Scholar : PubMed/NCBI
11	Perrier d’Hauterive S, Charlet-Renard C, Berndt S, et al: Human chorionic gonadotropin and growth factors at the embryonic-endometrial interface control leukemia inhibitory factor (LIF) and interleukin 6 (IL-6) secretion by human endometrial epithelium. Hum Reprod. 19:2633–2643. 2004.
12	Yang ZM, Chen DB, Le SP, et al: Differential hormonal regulation of leukemia inhibitory factor (LIF) in rabbit and mouse uterus. Mol Reprod Dev. 43:470–476. 1996. View Article : Google Scholar : PubMed/NCBI
13	Altmäe S, Martinez-Conejero JA, Salumets A, et al: Endometrial gene expression analysis at the time of embryo implantation in women with unexplained infertility. Mol Hum Reprod. 16:178–187. 2010.PubMed/NCBI
14	Strandell A, Thorburn J and Wallin A: The presence of cytokines and growth factors in hydrosalpingeal fluid. J Assist Reprod Genet. 21:241–247. 2004. View Article : Google Scholar : PubMed/NCBI
15	Copperman AB, Wells V, Luna M, et al: Presence of hydrosalpinx correlated to endometrial inflammatory response in vivo. Fertil Steril. 86:972–976. 2006. View Article : Google Scholar : PubMed/NCBI
16	Piccinni MP, Beloni L, Livi C, et al: Defective production of both leukaemia inhibitory factor and type 2 T-helper cytokines by decidual T cells in unexplained recurrent abortions. Nat Med. 4:1020–1024. 1998. View Article : Google Scholar : PubMed/NCBI
17	Rosen SD: Ligands for L-selectin: homing, inflammation, and beyond. Annu Rev Immunol. 22:129–156. 2004. View Article : Google Scholar : PubMed/NCBI
18	McEver RP: Selectins: lectins that initiate cell adhesion under flow. Curr Opin Cell Biol. 14:581–586. 2002. View Article : Google Scholar : PubMed/NCBI
19	Thiery JP: Cell adhesion in development: a complex signaling network. Curr Opin Genet Dev. 13:365–371. 2003. View Article : Google Scholar : PubMed/NCBI
20	Zhou Y, Genbacev O and Fisher SJ: The human placenta remodels the uterus by using a combination of molecules that govern vasculogenesis or leukocyte extravasation. Ann N Y Acad Sci. 995:73–83. 2003. View Article : Google Scholar : PubMed/NCBI
21	Genbacev OD, Prakobphol A, Foulk RA, et al: Trophoblast L-selectin-mediated adhesion at the maternal-fetal interface. Science. 299:405–408. 2003. View Article : Google Scholar : PubMed/NCBI
22	Shamonki MI, Kligman I, Shamonki JM, et al: Immunohistochemical expression of endometrial L-selectin ligand is higher in donor egg recipients with embryonic implantation. Fertil Steril. 86:1365–1375. 2006. View Article : Google Scholar : PubMed/NCBI