Morphological study of bone marrow to assess the effects of lead acetate on haemopoiesis and aplasia and the ameliorating role of Carica papaya extract

Tham,Ching  S.; Chakravarthi,Srikumar; Haleagrahara,Nagaraja; De Alwis,Ranjit

doi:10.3892/etm.2012.851

Morphological study of bone marrow to assess the effects of lead acetate on haemopoiesis and aplasia and the ameliorating role of Carica papaya extract

Authors:
- Ching S. Tham
- Srikumar Chakravarthi
- Nagaraja Haleagrahara
- Ranjit De Alwis
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Affiliations: Faculty of Medicine, University of Queensland, Brisbane, Australia, Department of Pathology, Faculty of Medicine, International Medical University, Kuala Lumpur, Malaysia, Faculty of Physiology, James Cook University, Townsville, Australia, Department of Community Medicine, Faculty of Medicine, International Medical University, Kuala Lumpur, Malaysia
Published online on: December 5, 2012 https://doi.org/10.3892/etm.2012.851
Pages: 648-652

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Abstract

Lead causes damage to the body by inducing oxidative stress. The sites of damage include the bone marrow, where marrow hypoplasia and osteosclerosis may be observed. Leaves of Carica papaya, which have antioxidant and haemopoietic properties, were tested against the effect of lead acetate in experimental rats. The rats were divided into 8 groups; control, lead acetate only, Carica papaya (50 mg and 200 mg), post‑treatment with Carica papaya (50 mg and 200 mg) following lead acetate administration and pre‑treatment with Carica papaya (50 mg and 200 mg) followed by lead acetate administration. The substances were administered for 14 days. The effects were evaluated by measuring protein carbonyl content (PCC) and glutathione content (GC) in the bone marrow. Histological changes in the bone marrow were also observed. The results showed that Carica papaya induced a significant reduction in the PCC activity and significantly increased the GC in the bone marrow. Carica papaya also improved the histology of the bone marrow compared with that of the lead acetate‑treated group. In summary, Carica papaya was effective against the oxidative damage caused by lead acetate in the bone marrow and had a stimulatory effect on haemopoiesis.

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1.	Bellinger DC: Very low lead exposures and children’s neurodevelopment. Curr Opin Pediatr. 20:172–177. 2008.
2.	El-Nekeety AA, El-Kady AA, Soliman MS, Hassan NS and Abdel-Wahhab MA: Protective effect of Aquilegia vulgaris (L.) against lead acetate-induced oxidative stress in rats. Food Chem Toxicol. 47:2209–2215. 2009.
3.	Sivaprasad R, Nagaraj M and Varalakshmi P: Combined efficacies of lipoic acid and 2,3-dimercaptosuccinic acid against lead-induced lipid peroxidation in rat liver. J Nutr Biochem. 15:18–23. 2004. View Article : Google Scholar : PubMed/NCBI
4.	Bradberry S and Vale A: Lead. Medicine. 31:56–57. 2003. View Article : Google Scholar
5.	Lee KH, Chen YS, Judson JP, Chakravarthi S, Sim YM and Er HM: The effect of water extracts of Euphorbia hirta on cartilage degeneration in arthritic rats. Malays J Pathol. 30:95–102. 2008.
6.	Haleagrahara N, Chakravarthi S, Kulur AB and Radhakrishnan A: Effects of chronic lead acetate exposure on bone marrow lipid peroxidation and antioxidant enzyme activities in rats. AJPP. 5:923–929. 2011.
7.	Haw KY, Chakravarthi S, Haleagrahara N and Rao M: Effects of Etlingera elatior extracts on lead acetate-induced testicular damage: A morphological and biochemical study. Exp Ther Med. 3:99–104. 2012.
8.	Jackie T, Haleagrahara H and Chakravarthi S: Antioxidant effects of Etlingera elatior flower extract against lead acetate-induced perturbations in free radical scavenging enzymes and lipid peroxidation in rats. BMC Res Notes. 4:672011.
9.	Owoyele BV, Adebukola OM, Funmilayo AA and Soladoye AO: Anti-inflammatory activities of ethanolic extract of Carica papaya leaves. Inflammopharmacology. 16:168–173. 2008. View Article : Google Scholar : PubMed/NCBI
10.	Otsuki N, Dang NH, Kumagai E, Kondo A, Iwata S and Morimoto C: Aqueous extract of Carica papaya leaves exhibits anti-tumor activity and immunomodulatory effects. J Ethnopharmacol. 127:760–767. 2010.
11.	Seigler DS, Pauli GF, Nahrstedt A and Leen R: Cyanogenic allosides and glucosides from Passiflora edulis and Carica papaya. Phytochemistry. 60:873–882. 2002. View Article : Google Scholar : PubMed/NCBI
12.	Sathasivam K, Ramanathan S, Mansor SM, Haris MR and Wernsdorfer WH: Thrombocyte counts in mice after the administration of papaya leaf suspension. Wien Klin Wochenschr. 121(Suppl 3): 19–22. 2009. View Article : Google Scholar : PubMed/NCBI
13.	Ahmad N, Fazal H, Ayaz M, Abbasi BH, Mohammad I and Fazal L: Dengue fever treatment with Carica papaya leaves extracts. Asian Pac J Trop Biomed. 330–333. 2011. View Article : Google Scholar
14.	Adonaylo VN and Oteiza PI: Lead intoxication: antioxidant defences and oxidative damage in rat brain. Toxicology. 135:77–85. 1999. View Article : Google Scholar : PubMed/NCBI
15.	Upasani CD, Khera A and Balaraman R: Effect of lead with Vitamins E, C, or Spirulina on malondialdehyde: conjugated dienes and hydroperoxides in rats. Indian J Exp Biol. 39:70–74. 2001.PubMed/NCBI
16.	Sharma V, Sharma A and Kansal L: The effect of oral administration of Allium sativum extracts on lead nitrate induced toxicity in male mice. Food Chem Toxicol. 48:928–936. 2010.
17.	Reznick AZ, Cross CE, Hu ML, et al: Modification of plasma proteins by cigarette smoke as measured by protein carbonyl formation. Biochem J. 286:607–611. 1992.PubMed/NCBI
18.	Haleagrahara N, Jackie T, Chakravarthi S, Rao M and Pasupathi T: Protective effects of Etlingera elatior extract on lead acetate-induced, changes in oxidative biomarkers in bone marrow of rats. Food Chem Toxicol. 48:2688–2694. 2010.
19.	Indran M, Mahmood AA and Kuppusamy UR: Protective effect of Carica papaya L leaf extract against alcohol induced acute gastric damage and blood oxidative stress in rats. West Indian Med J. 57:323–326. 2008.