Antifatigue effect of Gracilaria eucheumoides in mice

Shao,Jin‑Ting; Wang,Mei‑Yan; Zheng,Lu-Bin

doi:10.3892/etm.2013.1346

Antifatigue effect of Gracilaria eucheumoides in mice

Authors:
- Jin‑Ting Shao
- Mei‑Yan Wang
- Lu-Bin Zheng
View Affiliations

Affiliations: Physical and Military Training Department, Zhejiang University of Finance and Economics, Hangzhou, Zhejiang 310018, P.R. China, Sports Center, Zhejiang University of Finance and Economics Dongfang College, Hangzhou, Zhejiang 314408, P.R. China, Physical Education Department, Shanghai University of Sport, Shanghai 200438, P.R. China
Published online on: October 15, 2013 https://doi.org/10.3892/etm.2013.1346
Pages: 1512-1516

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Gracilaria eucheumoides Linn (Gracilariaceae; G. eucheumoides) is abundant in dietary fiber, which aids the clearance of excess cholesterol from the blood and maintains stable blood glucose levels. The aim of the present study was to investigate the antifatigue effect of G. eucheumoides in mice and the physiological and molecular mechanisms underlying this effect. Mice were randomly divided into four groups and three of the groups were administered different doses of G. eucheumoides extract. A loaded swimming test demonstrated that the swimming times of the low‑, medium‑ and high‑dose groups were longer than those of the control group. Examinations revealed that the liver and muscle glycogen, lactate dehydrogenase and blood glucose concentration levels of the treatment groups were higher than those of the control group (P<0.05). However, this was not the case for lactic acid concentration (P>0.05). Quantitative polymerase chain reaction showed that the gene expression levels of glucose transport protein 4 and AMP‑activated protein kinase in the medium‑dose group exhibited the largest increases, compared with the other treatment groups, and were 3.0- and 1.8‑fold higher than those in the control group, respectively. The results of the present study indicated that G. eucheumoides exerts an antifatigue effect on mice.

View Figures

View References

1	Bocanegra A, Bastida S, Benedí J, Nus M, Sánchez-Montero JM and Sánchez-Muniz FJ: Effect of seaweed and cholesterol-enriched diets on postprandial lipoproteinaemia in rats. Br J Nutr. 102:1728–1739. 2009. View Article : Google Scholar : PubMed/NCBI
2	Wada K, Nakamura K, Tamai Y, et al: Seaweed intake and blood pressure levels in healthy pre-school Japanese children. Nutr J. 10:832011. View Article : Google Scholar : PubMed/NCBI
3	Blokhuis TJ and Arts JJ: Bioactive and osteoinductive bone graft substitutes: definitions, facts and myths. Injury. 42(Suppl 2): S26–S29. 2011. View Article : Google Scholar : PubMed/NCBI
4	Ahmed HH, Hegazi MM, Abd-Alla HI, Eskander EF and Ellithey MS: Antitumour and antioxidant activity of some Red Sea seaweeds in Ehrlich ascites carcinoma in vivo. Z Naturforsch C. 66:367–376. 2011. View Article : Google Scholar : PubMed/NCBI
5	Wijesinghe WA and Jeon YJ: Exploiting biological activities of brown seaweed Ecklonia cava for potential industrial applications: a review. Int J Food Sci Nutr. 63:225–235. 2012. View Article : Google Scholar : PubMed/NCBI
6	Coura CO, de Araújo IW, Vanderlei ES, et al: Antinociceptive and anti-inflammatory activities of sulphated polysaccharides from the red seaweed Gracilaria cornea. Basic Clin Pharmacol Toxicol. 110:335–341. 2012. View Article : Google Scholar : PubMed/NCBI
7	Siqueira RC, da Silva MS, de Alencar DB, et al: In vivo anti-inflammatory effect of a sulfated polysaccharide isolated from the marine brown algae Lobophora variegata. Pharm Biol. 49:167–174. 2011. View Article : Google Scholar : PubMed/NCBI
8	Yuvaraj N, Kanmani P, Satishkumar R, Paari A, Pattukumar V and Arul V: Seagrass as a potential source of natural antioxidant and anti-inflammatory agents. Pharm Biol. 50:458–467. 2012.PubMed/NCBI
9	Xu HL, Kitajima C, Ito H, et al: Antidiabetic effect of polyphenols from brown alga Ecklonia kurome in genetically diabetic KK-A(y) mice. Pharm Biol. 50:393–400. 2012. View Article : Google Scholar : PubMed/NCBI
10	Lee YS, Shin KH, Kim BK and Lee S: Anti-diabetic activities of fucosterol from Pelvetia siliquosa. Arch Pharm Res. 27:1120–1122. 2004. View Article : Google Scholar : PubMed/NCBI
11	Albuquerque IR, Queiroz KC, Alves LG, Santos EA, Leite EL and Rocha HA: Heterofucans from Dictyota menstrualis have anticoagulant activity. Braz J Med Biol Res. 37:167–171. 2004.
12	Silva TM, Alves LG, de Queiroz KC, et al: Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora. Braz J Med Biol Res. 38:523–533. 2005. View Article : Google Scholar : PubMed/NCBI
13	Bogdanis GC: Effects of physical activity and inactivity on muscle fatigu. Front Physiol. 3:1422012. View Article : Google Scholar
14	Saiki T, Kawai T, Morita K, et al: Identification of marker genes for differential diagnosis of chronic fatigue syndrome. Mol Med. 14:599–607. 2008. View Article : Google Scholar : PubMed/NCBI
15	Finsterer J: Biomarkers of peripheral muscle fatigue during exercise. BMC Musculoskelet Disord. 13:2182012. View Article : Google Scholar : PubMed/NCBI
16	Takano R, Hayashi K and Hara S: Highly methylated agars with a high gel-melting point from the red seaweed, Gracilaria eucheumoides. Phytochemistry. 40:487–490. 1995. View Article : Google Scholar : PubMed/NCBI
17	Krishnaiah D, Sarbatly R, Prasad DMR and Bono A: Mineral content of some seaweeds from Sabah’s south China sea. Asian J Sci Res. 1:166–170. 2008.
18	Zhang X, Li H, Wu G, Ban S and Park H: Extraction of Eupatorium odoratum and its inhibition on toxic cyanobacteria. J Hainan Norm Uni (Nat Sci). 23:427–432. 2010.(In Chinese).
19	Jiménez-Escrig A, Gómez-Ordóñez E and Rupérez P: Seaweed as a source of novel nutraceuticals: sulfated polysaccharides and peptides. Adv Food Nutr Res. 64:325–337. 2011.PubMed/NCBI
20	Vera J, Castro J, Gonzalez A and Moenne A: Seaweed polysaccharides and derived oligosaccharides stimulate defense responses and protection against pathogens in plants. Mar Drugs. 9:2514–2525. 2011. View Article : Google Scholar : PubMed/NCBI
21	Lewis AS: Organic versus inorganic arsenic in herbal kelp supplements. Environ Health Perspect. 115:A5752007. View Article : Google Scholar : PubMed/NCBI
22	Sun XW, Weng HX and Qin YC: Release of bioactive active iodine in kelp. J Environ Sci (China). 17:241–244. 2005.PubMed/NCBI
23	Greenberg CC, Jurczak MJ, Danos AM and Brady MJ: Glycogen branches out: new perspectives on the role of glycogen metabolism in the integration of metabolic pathways. Am J Physiol Endocrinol Metab. 291:E1–E8. 2006. View Article : Google Scholar : PubMed/NCBI
24	Brook GA, Dubouchard H, Brown M, Sicurello JP and Butz CE: Role of mitochondrial lactate dehydrogenase and lactate oxidation in the intracellular lactate shuttle. Proc Natl Acad Sci USA. 96:1129–1134. 1999. View Article : Google Scholar : PubMed/NCBI
25	Scott CB: Contribution of anaerobic energy expenditure to whole body thermogenesis. Nutr Metab (Lond). 2:142005. View Article : Google Scholar : PubMed/NCBI
26	Heidrich F, Schotola H, Popov AF, et al: AMPK - activated protein kinase and its role in energy metabolism of the heart. Curr Cardiol Rev. 6:337–342. 2010. View Article : Google Scholar : PubMed/NCBI
27	Oakhill JS, Steel R, Chen ZP, et al: AMPK is a direct adenylate charge-regulated protein kinase. Science. 332:1433–1435. 2011. View Article : Google Scholar : PubMed/NCBI
28	Song H, Guang Y, Zhang L, Li K and Dong C: SPARC interacts with AMPK and regulates GLUT4 expression. Biochem Biophys Res Commun. 396:961–966. 2010. View Article : Google Scholar : PubMed/NCBI