Green tea catechin, epigallocatechin‑3‑gallate, attenuates the cell viability of human non‑small‑cell lung cancer A549 cells via reducing Bcl‑xL expression

  • Authors:
    • Jun‑Ichiro Sonoda
    • Ryuji Ikeda
    • Yasutaka Baba
    • Keiko Narumi
    • Akio Kawachi
    • Erisa Tomishige
    • Kazuya Nishihara
    • Yasuo Takeda
    • Katsushi Yamada
    • Keizo Sato
    • Toshiro Motoya
  • View Affiliations

  • Published online on: May 19, 2014     https://doi.org/10.3892/etm.2014.1719
  • Pages: 59-63
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Abstract

Clinical and epidemiological studies have indicated that the consumption of green tea has a number of beneficial effects on health. Epigallocatechin‑3‑gallate (EGCg), the major polyphenolic compound present in green tea, has received much attention as an active ingredient. Among the numerous promising profiles of EGCg, the present study focused on the anticancer effects. Apoptosis induced by EGCg and subsequent cell growth suppression have been demonstrated in a number of cell culture studies. However, the underlying mechanism of apoptotic cell death remains unclear. Thus, the aim of the present study was to identify the major molecule that mediates proapoptotic cell death by EGCg. The effect of EGCg on cell proliferation and the induction of mRNA that modulates apoptotic cell death was evaluated in the A549 human non‑small‑cell lung cancer cell line. In addition, morphological changes were assessed by microscopy in A549 cells that had been treated with 100 µM EGCg for 24 h. The MTT assay revealed that cell proliferation was significantly reduced by EGCg in a dose‑dependent manner (3‑100 µM). The mRNA expression level of B‑cell lymphoma‑extra large (Bcl‑xL) was decreased in A549 cells following 24 h incubation with 100 µM EGCg. Therefore, the results indicated that the inhibition of cell proliferation by EGCg may be achieved via suppressing the expression of the cell death‑inhibiting gene, Bcl‑xL.
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July-2014
Volume 8 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Sonoda JI, Ikeda R, Baba Y, Narumi K, Kawachi A, Tomishige E, Nishihara K, Takeda Y, Yamada K, Sato K, Sato K, et al: Green tea catechin, epigallocatechin‑3‑gallate, attenuates the cell viability of human non‑small‑cell lung cancer A549 cells via reducing Bcl‑xL expression. Exp Ther Med 8: 59-63, 2014.
APA
Sonoda, J., Ikeda, R., Baba, Y., Narumi, K., Kawachi, A., Tomishige, E. ... Motoya, T. (2014). Green tea catechin, epigallocatechin‑3‑gallate, attenuates the cell viability of human non‑small‑cell lung cancer A549 cells via reducing Bcl‑xL expression. Experimental and Therapeutic Medicine, 8, 59-63. https://doi.org/10.3892/etm.2014.1719
MLA
Sonoda, J., Ikeda, R., Baba, Y., Narumi, K., Kawachi, A., Tomishige, E., Nishihara, K., Takeda, Y., Yamada, K., Sato, K., Motoya, T."Green tea catechin, epigallocatechin‑3‑gallate, attenuates the cell viability of human non‑small‑cell lung cancer A549 cells via reducing Bcl‑xL expression". Experimental and Therapeutic Medicine 8.1 (2014): 59-63.
Chicago
Sonoda, J., Ikeda, R., Baba, Y., Narumi, K., Kawachi, A., Tomishige, E., Nishihara, K., Takeda, Y., Yamada, K., Sato, K., Motoya, T."Green tea catechin, epigallocatechin‑3‑gallate, attenuates the cell viability of human non‑small‑cell lung cancer A549 cells via reducing Bcl‑xL expression". Experimental and Therapeutic Medicine 8, no. 1 (2014): 59-63. https://doi.org/10.3892/etm.2014.1719