Protective effects of quercetin and hyperoside on renal fibrosis in rats with unilateral ureteral obstruction

Yan,Yang; Feng,Yuan; Li,Wei; Che,Jian-Ping; Wang,Guang-Chun; Liu,Min; Zheng,Jun-Hua

doi:10.3892/etm.2014.1841

Protective effects of quercetin and hyperoside on renal fibrosis in rats with unilateral ureteral obstruction

Authors:
- Yang Yan
- Yuan Feng
- Wei Li
- Jian-Ping Che
- Guang-Chun Wang
- Min Liu
- Jun-Hua Zheng
View Affiliations

Affiliations: Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai, P.R. China, Department of Nephrology, Nanjing University Affiliated Drum Tower Hospital, Nanjing, Jiangsu, P.R. China
Published online on: July 14, 2014 https://doi.org/10.3892/etm.2014.1841
Pages: 727-730

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Prevention of renal fibrosis is an important therapeutic strategy in the treatment of obstructive nephropathy. The purpose of the present study was to identify whether the combination of two natural plant‑derived flavanoids, quercetin and hyperoside (QH), could inhibit renal fibrosis in the model of unilateral ureteral obstruction (UUO) in rats. QH mixtures (1:1) were fed to Wistar rats, and UUO ligation was performed on all the rats with the exception of the sham group. Masson's trichrome staining was used for interstitial fibrosis, while immunohistochemistry and western blot analysis were used to detect the expression of α‑smooth muscle actin (SMA) and fibronectin (FN). In the QH group, the expression of SMA and FN was significantly lower than that in the untreated UUO group. In addition, QH administration significantly inhibited the SMA and FN expression of mesangial cells induced by interleukin-1β. Consequently, it was evident that combinational QH therapy prevented UUO‑induced renal fibrosis.Based on these findings, the combinatorial intervention of phytomedicine may present an improved treatment strategy for renal fibrotic disease.

View Figures

View References

1	Seikaly MG, Ho PL, Emmett L, et al: Chronic renal insufficiency in children: the 2001 Annual Report of the NAPRTCS. Pediatr Nephrol. 18:796–804. 2003. View Article : Google Scholar : PubMed/NCBI
2	Bek K, Akman S, Bilge I, et al: Chronic kidney disease in children in Turkey. Pediatr Nephrol. 24:797–806. 2009. View Article : Google Scholar : PubMed/NCBI
3	Acikgoz Y, Can B, Bek K, et al: The effect of simvastatin and erythropoietin on renal fibrosis in rats with unilateral ureteral obstruction. Ren Fail. 36:252–257. 2014. View Article : Google Scholar : PubMed/NCBI
4	Sagar SK, Zhang C, Guo Q, et al: Role of expression of endothelin-1 and angiotensin-II and hypoxia-inducible factor-1α in the kidney tissues of patients with diabetic nephropathy. Saudi J Kidney Dis Transpl. 24:959–964. 2013.
5	Carew RM, Wang B and Kantharidis P: The role of EMT in renal fibrosis. Cell Tissue Res. 347:103–116. 2012. View Article : Google Scholar : PubMed/NCBI
6	Chevalier RL, Forbes MS and Thornhill BA: Ureteral obstruction as a model of renal interstitial fibrosis and obstructive nephropathy. Kidney Int. 75:1145–1152. 2009. View Article : Google Scholar : PubMed/NCBI
7	Shen Y, Ward NC, Hodgson JM, et al: Dietary quercetin attenuates oxidant-induced endothelial dysfunction and atherosclerosis in apolipoprotein E knockout mice fed a high-fat diet: a critical role for heme oxygenase-1. Free Radic Biol Med. 65:908–915. 2013. View Article : Google Scholar : PubMed/NCBI
8	Kobori M, Masumoto S, Akimoto Y and Oike H: Chronic dietary intake of quercetin alleviates hepatic fat accumulation associated with consumption of a Western-style diet in C57/BL6J mice. Mol Nutr Food Res. 55:530–540. 2011. View Article : Google Scholar : PubMed/NCBI
9	Boesch-Saadatmandi C, Wagner AE, Wolffram S and Rimbach G: Effect of quercetin on inflammatory gene expression in mice liver in vivo-role of redox factor 1, miRNA-122 and miRNA-125b. Pharmacol Res. 65:523–530. 2012. View Article : Google Scholar : PubMed/NCBI
10	Choi YJ, Arzuaga X, Kluemper CT, et al: Quercetin blocks caveolae-dependent pro-inflammatory responses induced by co-planar PCBs. Environ Int. 36:931–934. 2010. View Article : Google Scholar : PubMed/NCBI
11	Noorafshan A, Karbalay-Doust S and Poorshahid M: Stereological survey of the ameliorative effects of sulforaphane and quercetin on renal tissue in unilateral ureteral obstruction in rats. Acta Clin Croat. 51:555–562. 2012.PubMed/NCBI
12	Li W, Liu M, Xu YF, et al: Combination of quercetin and hyperoside has anticancer effects on renal cancer cells through inhibition of oncogenic microRNA-27a. Oncol Rep. 31:117–124. 2014.PubMed/NCBI
13	Tan R, Zhang J, Tan X, et al: Downregulation of SnoN expression in obstructive nephropathy is mediated by an enhanced ubiquitin-dependent degradation. J Am Soc Nephrol. 17:2781–2791. 2006. View Article : Google Scholar : PubMed/NCBI
14	Su J, Yin LP, Zhang X, et al: Chronic allograft nephropathy in rats is improved by the intervention of rhein. Transplant Proc. 45:2546–2552. 2013. View Article : Google Scholar : PubMed/NCBI
15	Papiez MA, Cierniak A, Krzysciak W, et al: The changes of antioxidant defense system caused by quercetin administration do not lead to DNA damage and apoptosis in the spleen and bone marrow cells of rats. Food Chem Toxicol. 46:3053–3058. 2008. View Article : Google Scholar : PubMed/NCBI
16	Lu NH, Chen C, He YJ, et al: Effects of quercetin on hemoglobin-dependent redox reactions: relationship to iron-overload rat liver injury. J Asian Nat Prod Res. 15:1265–1276. 2013. View Article : Google Scholar : PubMed/NCBI
17	Chidambaram U, Pachamuthu V, Natarajan S, et al: In vitro evaluation of free radical scavenging activity of Codariocalyx motorius root extract. Asian Pac J Trop Med. 6:188–194. 2013. View Article : Google Scholar : PubMed/NCBI
18	Lee S, Park HS, Notsu Y, et al: Effects of hyperin, isoquercitrin and quercetin on lipopolysaccharide-induced nitrite production in rat peritoneal macrophages. Phytother Res. 22:1552–1556. 2008. View Article : Google Scholar : PubMed/NCBI
19	Kicel A and Wolbiś M: Phenolic content and DPPH radical scavenging activity of the flowers and leaves of Trifolium repens. Nat Prod Commun. 8:99–102. 2013.PubMed/NCBI
20	Li ZL, Hu J, Li YL, et al: The effect of hyperoside on the functional recovery of the ischemic/reperfused isolated rat heart: potential involvement of the extracellular signal-regulated kinase 1/2 signaling pathway. Free Radic Biol Med. 57:132–140. 2013. View Article : Google Scholar
21	Ito K, Chen J, El Chaar M, et al: Renal damage progresses despite improvement of renal function after relief of unilateral ureteral obstruction in adult rats. Am J Physiol Renal Physiol. 287:F1283–F1293. 2004. View Article : Google Scholar : PubMed/NCBI
22	Tan R, He W, Lin X, et al: Smad ubiquitination regulatory factor-2 in the fibrotic kidney: regulation, target specificity, and functional implication. Am J Physiol Renal Physiol. 294:F1076–F1083. 2008. View Article : Google Scholar : PubMed/NCBI
23	Wu T, Zhang B, Ye F and Xiao Z: A potential role for caveolin-1 in VEGF-induced fibronectin upregulation in mesangial cells: involvement of VEGFR2 and Src. Am J Physiol Renal Physiol. 304:F820–F830. 2013. View Article : Google Scholar : PubMed/NCBI