Expression levels of TWIST1 are associated with the clinicopathological stage of B‑cell non‑Hodgkin lymphoma

  • Authors:
    • Cundong Jia
    • Liping Liang
    • Lili Yang
    • Feng Zhao
    • Jingping Bai
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  • Published online on: September 15, 2014     https://doi.org/10.3892/etm.2014.1962
  • Pages: 1489-1493
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Abstract

The aim of the present study was to investigate the expression level of TWIST1 in B‑cell non‑Hodgkin lymphoma (BNHL) and its association with the clinicopathological characteristics of BNHL. Expression levels of TWIST1 were analyzed in patients with BNHL (n=45) and lymphadenosis (n=21) using immunohistochemical staining and western blot analysis. In addition, the mRNA expression levels of TWIST1 in the peripheral blood were detected by fluorescent quantitative polymerase chain reaction. The positive rate of TWIST1 expression in the BNHL tissue was 82.2%, which was significantly higher compared with the lymphadenosis tissue (5%; P<0.05). In addition, the protein expression level of TWIST1 in the BNHL tissue was higher compared with the lymphadenosis tissue. TWIST1 expression was also higher in stage III/IV BNHL tissues than in stage I/II tissues (P<0.05). The tissues were staged following the Ann Arbor system. Furthermore, the mRNA expression level of TWIST1 in the peripheral blood of the BNHL tissue (3.03±0.03) was higher compared with the lymphadenosis tissue, and the mRNA expression level of TWIST1 was higher in stage III/IV (4.41±0.12) tissues than in stage I/II BNHL (2.03±0.08) tissues. In conclusion, TWIST1 expression was higher in the tissue and peripheral blood of patients with BNHL when compared with those with lymphadenosis. Thus, TWIST1 expression was associated with the clinicopathological stage of BNHL.
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November-2014
Volume 8 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Jia C, Liang L, Yang L, Zhao F and Bai J: Expression levels of TWIST1 are associated with the clinicopathological stage of B‑cell non‑Hodgkin lymphoma. Exp Ther Med 8: 1489-1493, 2014.
APA
Jia, C., Liang, L., Yang, L., Zhao, F., & Bai, J. (2014). Expression levels of TWIST1 are associated with the clinicopathological stage of B‑cell non‑Hodgkin lymphoma. Experimental and Therapeutic Medicine, 8, 1489-1493. https://doi.org/10.3892/etm.2014.1962
MLA
Jia, C., Liang, L., Yang, L., Zhao, F., Bai, J."Expression levels of TWIST1 are associated with the clinicopathological stage of B‑cell non‑Hodgkin lymphoma". Experimental and Therapeutic Medicine 8.5 (2014): 1489-1493.
Chicago
Jia, C., Liang, L., Yang, L., Zhao, F., Bai, J."Expression levels of TWIST1 are associated with the clinicopathological stage of B‑cell non‑Hodgkin lymphoma". Experimental and Therapeutic Medicine 8, no. 5 (2014): 1489-1493. https://doi.org/10.3892/etm.2014.1962