Expression of porcine protegrin-1 in Pichia pastoris and its anticancer activity in vitro

Corrigendum in: /10.3892/etm.2022.11713

  • Authors:
    • Mingfu Niu
    • Shumao Chai
    • Xiaoyan You
    • Wenhui Wang
    • Cuili Qin
    • Qiang Gong
    • Tingting Zhang
    • Peng Wan
  • View Affiliations

  • Published online on: January 22, 2015     https://doi.org/10.3892/etm.2015.2202
  • Pages: 1075-1079
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Abstract

Protegrin‑1 (PG‑1), a β‑hairpin antimicrobial peptide (AMP), is amongst the shortest AMPs in sequence length while remaining active against a variety of microorganisms. The aim of this study was produce recombinant PG‑1 and investigate its anticancer activity. A DNA sequence encoding the mature PG‑1, fused with a 6His‑tag, was cloned into the pPICZα‑A vector and transformed into Pichia pastoris. Expression was induced following culture for ~96 h with 1% methanol at 28˚C, and ~15.6 mg PG‑1 was expressed in 100 ml culture medium. Following purification using a Ni‑chelating Sepharose column, ~20 mg pure active PG‑1 was obtained from 500 ml culture broth supernatant. The expressed PG‑1/6His exhibited strong dose‑ and time‑dependent anticancer activity against HepG2 cells in vitro.
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March-2015
Volume 9 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Niu M, Chai S, You X, Wang W, Qin C, Gong Q, Zhang T and Wan P: Expression of porcine protegrin-1 in Pichia pastoris and its anticancer activity in vitro Corrigendum in /10.3892/etm.2022.11713. Exp Ther Med 9: 1075-1079, 2015.
APA
Niu, M., Chai, S., You, X., Wang, W., Qin, C., Gong, Q. ... Wan, P. (2015). Expression of porcine protegrin-1 in Pichia pastoris and its anticancer activity in vitro Corrigendum in /10.3892/etm.2022.11713. Experimental and Therapeutic Medicine, 9, 1075-1079. https://doi.org/10.3892/etm.2015.2202
MLA
Niu, M., Chai, S., You, X., Wang, W., Qin, C., Gong, Q., Zhang, T., Wan, P."Expression of porcine protegrin-1 in Pichia pastoris and its anticancer activity in vitro Corrigendum in /10.3892/etm.2022.11713". Experimental and Therapeutic Medicine 9.3 (2015): 1075-1079.
Chicago
Niu, M., Chai, S., You, X., Wang, W., Qin, C., Gong, Q., Zhang, T., Wan, P."Expression of porcine protegrin-1 in Pichia pastoris and its anticancer activity in vitro Corrigendum in /10.3892/etm.2022.11713". Experimental and Therapeutic Medicine 9, no. 3 (2015): 1075-1079. https://doi.org/10.3892/etm.2015.2202