Postconditioning with simvastatin decreases myocardial injury in rats following acute myocardial ischemia

Yao,Heng‑Chen; Yang,Lan‑Ju; Han,Qian‑Feng; Wang,Lan‑Hua; Wu,Lei; Zhang,Chun‑Yan; Tian,Ke‑Li; Zhang,Mei

doi:10.3892/etm.2015.2273

Postconditioning with simvastatin decreases myocardial injury in rats following acute myocardial ischemia

Authors:
- Heng‑Chen Yao
- Lan‑Ju Yang
- Qian‑Feng Han
- Lan‑Hua Wang
- Lei Wu
- Chun‑Yan Zhang
- Ke‑Li Tian
- Mei Zhang
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Affiliations: Department of Cardiology, Qilu Hospital of Shandong University School of Medicine, Jinan, Shandong 250012, P.R. China, Department of Cardiology, Liaocheng People's Hospital of Taishan Medical University, Liaocheng, Shandong 252000, P.R. China, Department of Biochemistry and Molecular Biology, Shandong University School of Medicine, Jinan, Shandong 250012 P.R. China
Published online on: February 6, 2015 https://doi.org/10.3892/etm.2015.2273
Pages: 1166-1170
Copyright: © Yao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate whether postconditioning with simvastatin attenuated myocardial ischemia reperfusion injury by inhibiting the expression of high mobility group box 1 (HMGB1) in rat myocardium following acute myocardial ischemia. In total, 30 male Sprague‑Dawley rats were divided into sham operation (sham; n=10), acute myocardial infarction (AMI; n=10) and simvastatin (sim; n=10) groups. The AMI and sim groups were subjected to ischemia for 30 min, followed by reperfusion for 180 min. The rats in the sim group were administered 20 mg/kg simvastatin intravenously 5 min prior to reperfusion. Subsequently, the infarct size, serum cardiac troponin (c‑TnI), tumor necrosis factor (TNF)‑α and myocardial malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity were measured. Western blot analysis was used to detect the protein expression of HMGB1. Postconditioning with simvastatin was shown to decrease the infarct size and HMGB1 expression levels in the myocardium following AMI (P<0.05). In addition, postconditioning with simvastatin not only decreased the serum levels of c‑TnI and TNF‑α (P<0.05), but also inhibited the increase in MDA levels and the reduction in SOD activity (P<0.05). Therefore, postconditioning with simvastatin was shown to attenuate myocardial injury. The underlying mechanism may be associated with the downregulation of HMGB1 expression in the ischemic myocardium.

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