Humulus japonicus extract exhibits antioxidative and anti‑aging effects via modulation of the AMPK‑SIRT1 pathway

Sung,Bokyung; Chung,Ji Won; Bae,Ha Ram; Choi,Jae Sue; Kim,Cheol Min; Kim,Nam Deuk

doi:10.3892/etm.2015.2302

Humulus japonicus extract exhibits antioxidative and anti‑aging effects via modulation of the AMPK‑SIRT1 pathway

Authors:
- Bokyung Sung
- Ji Won Chung
- Ha Ram Bae
- Jae Sue Choi
- Cheol Min Kim
- Nam Deuk Kim
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Affiliations: Department of Pharmacy, Molecular Inflammation Research Center for Aging Intervention, Pusan National University, Busan 609‑735, Republic of Korea, Faculty of Food Science and Biotechnology, Pukyong National University, Busan 608‑737, Republic of Korea, Department of Biochemistry, School of Medicine, Pusan National University, Yangsan, Gyeongsangnam‑do 626‑770, Republic of Korea
Published online on: February 17, 2015 https://doi.org/10.3892/etm.2015.2302
Pages: 1819-1826

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Abstract

The perennial herb, Humulus japonicus, has been previously described as possessing potential antituberculosis and anti‑inflammatory properties. In the present study, the anti‑aging activity of ethanol extracts from the leaves of H. japonicus (HJE) was evaluated in yeast and human fibroblast cells. In addition, the antioxidant activity of HJE was analyzed using free radical scavenging assays. Furthermore, the mechanism underlying the hypothesized HJE‑associated extension of lifespan was investigated, and the results indicated that HJE was able to extend the lifespan of yeast cells. Further experiments demonstrated that HJE upregulated the longevity‑associated proteins, sirtuin 1 and AMP‑activated protein kinase, and effectively inhibited the generation of reactive oxygen species (ROS). In addition, the antioxidative potential of the active constituents of HJE, including luteolin, luteolin 7‑glycoside, quercetin and quercitrin, was evaluated and the results demonstrated that these flavonoids were able to scavenge ROS in cell‑free and intracellular systems. In summary, the results revealed that HJE possessed the potential for antioxidative activity; however, further in vivo investigations are required with the aim of developing safe, high‑efficacy anti‑aging agents.

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