Effect of elastin-derived peptides on the production of tissue inhibitor of metalloproteinase-1, -2, and -3 and the ratios in various endothelial cell lines

  • Authors:
    • Krzysztof Siemianowicz
    • Wirginia Likus
    • Tomasz Francuz
    • Wojciech Garczorz
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  • Published online on: April 17, 2015     https://doi.org/10.3892/etm.2015.2429
  • Pages: 2245-2250
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Abstract

Tissue inhibitors of metalloproteinases (TIMPs) control the activity of metalloproteinases. Elastin-derived peptides (EDPs) are generated as a result of the degradation of elastin fibers. The EDPs bind to the elastin receptor and exert numerous biological effects. The aim of the present study was to compare the production of TIMP‑1, TIMP‑2 and TIMP‑3 and their ratios in human endothelial cells (ECs) derived from three clinically important vascular localizations (coronary arteries, aorta and iliac artery), and evaluate the influence of a well‑known EDP, κ‑elastin. The highest concentration of TIMP‑1 was identified in the aortic ECs, while the lowest concentration was observed in the ECs derived from the coronary artery. The opposite pattern was observed for TIMP‑2 production. When the TIMP‑3 concentration was analyzed in the three EC lines, no statistically significant differences were observed. Application of κ‑elastin was found to decrease the TIMP‑1 concentration in the aortic ECs, while an increase in the TIMP‑1 concentration was observed in the ECs derived from the iliac artery. The most significant decrease in TIMP‑2 concentration following κ‑elastin administration was observed in the ECs obtained from the coronary arteries. Furthermore, the highest concentration of κ‑elastin resulted in an increase in TIMP‑3 production in the ECs derived from the coronary arteries. The following ratios of the TIMP concentrations were calculated: TIMP‑1/TIMP‑2, TIMP‑1/TIMP‑3 and TIMP‑2/TIMP‑3. Each ratio presented different values for the ECs obtained from the various localizations. In the majority of cases, the addition of κ‑elastin did not significantly change these proportions. Therefore, these indicators may be characteristic features that can be used to describe ECs in various clinically important vascular localizations.
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June-2015
Volume 9 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Siemianowicz K, Likus W, Francuz T and Garczorz W: Effect of elastin-derived peptides on the production of tissue inhibitor of metalloproteinase-1, -2, and -3 and the ratios in various endothelial cell lines. Exp Ther Med 9: 2245-2250, 2015.
APA
Siemianowicz, K., Likus, W., Francuz, T., & Garczorz, W. (2015). Effect of elastin-derived peptides on the production of tissue inhibitor of metalloproteinase-1, -2, and -3 and the ratios in various endothelial cell lines. Experimental and Therapeutic Medicine, 9, 2245-2250. https://doi.org/10.3892/etm.2015.2429
MLA
Siemianowicz, K., Likus, W., Francuz, T., Garczorz, W."Effect of elastin-derived peptides on the production of tissue inhibitor of metalloproteinase-1, -2, and -3 and the ratios in various endothelial cell lines". Experimental and Therapeutic Medicine 9.6 (2015): 2245-2250.
Chicago
Siemianowicz, K., Likus, W., Francuz, T., Garczorz, W."Effect of elastin-derived peptides on the production of tissue inhibitor of metalloproteinase-1, -2, and -3 and the ratios in various endothelial cell lines". Experimental and Therapeutic Medicine 9, no. 6 (2015): 2245-2250. https://doi.org/10.3892/etm.2015.2429