Expression levels of cytokines and chemokines increase in human peripheral blood mononuclear cells stimulated by activation of the Toll-like receptor 5 pathway
- Authors:
- Yu Ma
- Li Zhang
- Quansheng Li
-
View Affiliations
Affiliations: Department of Thyroid and Breast Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Laboratory of Pathology, Department of Pathology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Biliary Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
- Published online on: December 4, 2015 https://doi.org/10.3892/etm.2015.2914
-
Pages:
588-592
Metrics:
Total
Views: 0 (Spandidos Publications: | PMC Statistics:
)
Metrics:
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics:
)
This article is mentioned in:
Abstract
Recognition of pathogen-associated molecular patterns by Toll-like receptors (TLRs) activates innate and adaptive immune responses. Among the 11 members of the human TLR family, TLR-5 is known to play an important role in the defense against bacterial invasion by binding to flagellin, a conserved component of bacteria. Previous studies have demonstrated that the activation of TLR‑5 induces the expression of interleukin (IL)‑10, IL‑12 and interferon‑β. However, the aim of the present study was to analyze the expression of a wider range of immune‑related molecules upon stimulation with a TLR‑5 agonist. Following isolation from healthy volunteers, peripheral blood mononuclear cells (PBMCs) were stimulated with flagellin, a TLR‑5 agonist. At 4 h after stimulation, quantitative polymerase chain reaction (PCR) and an antibody chip array were conducted to determine the mRNA expression levels of immune molecules and the protein secretion of immune molecules in the supernatant, respectively. The PCR results revealed that activation of TLR‑5 significantly influenced the expression of a number of important molecules. In addition, the antibody chip array demonstrated the induction (IL‑8) and inhibition [monocyte chemoattractant protein (MCP)‑1, MCP‑3 and macrophage inflammatory protein‑1α) of protein secretion following TLR‑5 stimulation. Therefore, the present study demonstrated the importance of TLR‑5 in regulating the biological function of PBMCs. In the future, research should focus on the roles of the candidate molecules in TLR‑5-mediating functions.
View References
|
1
|
Akira S, Uematsu S and Takeuchi O:
Pathogen recognition and innate immunity. Cell. 124:783–801. 2006.
View Article : Google Scholar : PubMed/NCBI
|
|
2
|
Takeda K, Kaisho T and Akira S: Toll-like
receptors. Annu Rev Immunol. 21:335–376. 2003. View Article : Google Scholar : PubMed/NCBI
|
|
3
|
Ozinsky A, Underhill DM, Fontenot JD, et
al: The repertoire for pattern recognition of pathogens by the
innate immune system is defined by cooperation between Toll-like
receptors. Proc Natl Acad Sci USA. 97:13766–13771. 2000. View Article : Google Scholar : PubMed/NCBI
|
|
4
|
Iwasaki A and Medzhitov R: Regulation of
adaptive immunity by the innate immune system. Science.
327:291–295. 2010. View Article : Google Scholar : PubMed/NCBI
|
|
5
|
Merio A, Calcaterra C, Mènard S and
Balsari A: Cross-talk between Toll-like receptors 5 and 9 on
activation of human immune responses. J Leukoc Biol. 82:509–518.
2007. View Article : Google Scholar : PubMed/NCBI
|
|
6
|
Akira S and Takeda K: Toll-like receptor
signalling. Nat Rev Immunol. 4:499–511. 2004. View Article : Google Scholar : PubMed/NCBI
|
|
7
|
Beutler B: Inferences questions and
possibilities in Toll-like receptor signaling. Nature. 430:257–263.
2004. View Article : Google Scholar : PubMed/NCBI
|
|
8
|
Balander JM and Medzhitov R: Regulation of
phagosome maturation by signals from Toll-like receptors. Science.
304:1014–1018. 2004. View Article : Google Scholar : PubMed/NCBI
|
|
9
|
Hawn TR, Verbon A, Letinga KD, et al: A
common dominant TLR5 stop codon polymorphism abolishes flagellin
signaling and is associated with susceptibility to legionnaires'
disease. J Exp Med. 198:1563–1572. 2003. View Article : Google Scholar : PubMed/NCBI
|
|
10
|
Hayashi F, Smith KD, Ozinsky A, et al: The
innate immune response to bacterial flagellin is mediated by
Toll-like receptor 5. Nature. 410:1099–1103. 2001. View Article : Google Scholar : PubMed/NCBI
|
|
11
|
Andersen-Nissen E, Smith KD, Strobe KL, et
al: Evasion of Toll-like receptor 5 by flagellated bacteria. Proc
Natl Acad Sci USA. 102:9247–9252. 2005. View Article : Google Scholar : PubMed/NCBI
|
|
12
|
Lin Y, Zhang L, Cai AX, Lee M, Zhang W,
Neuberg D, Canning CM, Soiffer RJ, Alyea EP, Ritz J, Hacohen N,
Means TK and Wu CJ: Effective posttransplant antitumor immunity is
associated with TLR-stimulating nucleic acid-immunoglobulin
complexes in humans. J Clin Invest. 121:1574–1584. 2011. View Article : Google Scholar : PubMed/NCBI
|
