Early application of negative pressure wound therapy to acute wounds contaminated with Staphylococcus aureus: An effective approach to preventing biofilm formation

Li,Tongtong; Zhang,Lihai; Han,Li; Wang,Guoqi; Yin,Peng; Li,Zhirui; Zhang,Licheng; Guo,Qi; Liu,Daohong; Tang,Peifu

doi:10.3892/etm.2016.3008

Early application of negative pressure wound therapy to acute wounds contaminated with Staphylococcus aureus: An effective approach to preventing biofilm formation

Authors:
- Tongtong Li
- Lihai Zhang
- Li Han
- Guoqi Wang
- Peng Yin
- Zhirui Li
- Licheng Zhang
- Qi Guo
- Daohong Liu
- Peifu Tang
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Affiliations: Department of Clinical Medicine, Medical College, Nankai University, Tianjin 300071, P.R. China, Department of Orthopedics, Chinese PLA General Hospital, Beijing 100853, P.R. China, Center for Hospital Infection Control, Chinese PLA Institute for Disease Control and Prevention, Beijing 100071, P.R. China, Department of Orthopedics, The 309th Hospital of PLA, Beijing 100091, P.R. China
Published online on: January 20, 2016 https://doi.org/10.3892/etm.2016.3008
Pages: 769-776
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Negative pressure wound therapy (NPWT) has been demonstrated to be effective at preventing biofilm-associated infections; however, its role in biofilm prevention is unknown. The present study evaluated the effect of NPWT on biofilm prevention when rapidly initiated following wound contamination. Full‑thickness dermal wounds (8 mm) were created in rabbit ears and inoculated with green fluorescent protein‑labeled Staphylococcus aureus (S. aureus). At 6 h following inoculation, continuous NPWT at ‑125 mmHg was initiated, with the wounds on the contralateral ear left untreated in order to serve as self‑controls. S. aureus rapidly formed mature biofilms in the wound beds post-inoculation, with a persistent bacterial burden of ~105‑107 colony‑forming units (CFUs)/wound and impaired wound healing. Compared with the untreated group, NPWT resulted in a significant reduction in biofilm matrix, which was verified by scanning electron microscopy and epifluorescence. A reduction in bacterial counts followed (P<0.05) with ~103 CFUs/wound on postoperative day 13 and improvement in all healing parameters (P<0.05) relative to control wounds. The results of the present investigation suggest that NPWT is an effective strategy to impeding the formation of S. aureus wound biofilms when initiated rapidly following bacterial contamination. The early application of NPWT, aimed at biofilm prevention, may improve wound care.

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