Preventive activity of banana peel polyphenols on CCl4-induced experimental hepatic injury in Kunming mice

Wang,Rui; Feng,Xia; Zhu,Kai; Zhao,Xin; Suo,Huayi

doi:10.3892/etm.2016.3155

Preventive activity of banana peel polyphenols on CCl4-induced experimental hepatic injury in Kunming mice

Authors:
- Rui Wang
- Xia Feng
- Kai Zhu
- Xin Zhao
- Huayi Suo
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Affiliations: Chongqing Collaborative Innovation Center of Functional Food, Chongqing University of Education, Chongqing 400067, P.R. China, Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China, College of Food Science, Southwest University, Chongqing 400715, P.R. China
Published online on: March 11, 2016 https://doi.org/10.3892/etm.2016.3155
Pages: 1947-1954

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Abstract

The aim of the present study was to evaluate the preventive effects of banana peel polyphenols (BPPs) against hepatic injury. Mice were divide into normal, control, 100 mg/kg and 200 mg/kg banana peel polyphenol and silymarin groups. All the mice except normal mice were induced with hepatic damage using CCl4. The serum and tissue levels of mice were determined by a kit and the tissues were further examined by reverse transcription-quantitative polymerase chain reaction (RT‑qPCR) and western blot analysis. BPPs reduced the serum levels of aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase in a CCl4‑induced mouse model of hepatic injury. Furthermore, BPPs reduced the levels of malondialdehyde and triglyceride, while increasing glutathione levels in the serum and liver tissues of mice. In addition, the effects of 200 mg/kg treatment were more evident, and these effects were comparable to those of the drug silymarin. Serum levels of the cytokines, interleukin (IL)‑6, IL‑12, tumor necrosis factor (TNF)‑α and interferon-γ, were reduced in the mice treated with BPPs compared with injury control group mice, and these levels were comparable to those of the normal and silymarin‑treated groups. Histopathological examination indicated that BPPs were able to reduce the extent of CCl4‑induced liver tissue injury and protect the liver cells. Furthermore, the mRNA and protein expression levels of the inflammation‑associated factors cyclooxygenase-2, nitric oxide synthase, TNF‑α and IL‑1β were reduced in mice treated with BPPs compared with the control group mice. Mice that received 200 mg/kg BPP exhibited reduced expression levels of these factors compared with mice that received 100 mg/kg BPP. In conclusion, the results of the present study suggested that BPPs exert a good preventive effect against hepatic injury.

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1	Sang LW, Li L and Zheng FC: Study on the comprehensive utilization of banana by-product. Hei Long Jiang Nong Ye Ke Xue. 4:96–98. 2006.(In Chinese).
2	Liu S, Tang YF, Zhao QL, Li Y, Chen J, Li Ml, Zhao H and Zhou Y: Extraction of polyphenols from banana peel and anti-fungal effect research. Hu Nan Sheng Chang Sha Shi Xian Jia Hu Hu Nan Shi Fan Da Xue Yi Xue. 6:12–14. 2009.(In Chinese).
3	Kaviarasan S and Anuradha CV: Fenugreek (Trigonella foenum-graecum) seed polyphenols protect liver from alcohol toxicity: a role on hepatic detoxification system and apoptosis. Pharmazie. 62:299–304. 2007.PubMed/NCBI
4	Kang MC, Kang SM, Ahn G, Kim KN, Kang N, Samarakoon KW, Oh MC, Lee JS and Jeon YJ: Protective effect of a marine polyphenol, dieckol against carbon tetrachloride-induced acute liver damage in mouse. Environ Toxicol Pharmacol. 35:517–523. 2013. View Article : Google Scholar : PubMed/NCBI
5	Sebai H, Sani M, Yacoubi MT, Aouani E, Ghanem-Boughanmi N and Ben-Attia M: Resveratrol, a red wine polyphenol, attenuates lipopolysaccharide-induced oxidative stress in rat liver. Ecotoxicol Environ Saf. 73:1078–1083. 2010. View Article : Google Scholar : PubMed/NCBI
6	Cauwels A and Brouckaert P: Survival of TNF toxicity: dependence on caspases and NO. Arch Biochem Biophys. 462:132–139. 2007. View Article : Google Scholar : PubMed/NCBI
7	Zhao X: Hawk tea (Litsea coreana Levl. var. lanuginose) attenuates CCl₄-induced hepatic damage in Sprague-Dawley rats. Exp Ther Med. 5:555–560. 2013.PubMed/NCBI
8	Weber LW, Boll M and Stampfl A: Hepatotoxicity and mechanism of action of haloalkanes: Carbon tetrachloride as a toxicological model. Crit Rev Toxicol. 33:105–136. 2003. View Article : Google Scholar : PubMed/NCBI
9	Shang D, Xu XQ and Wu WJ: Study of extraction of polyphenols from different states banana peels. Food Sci Technol. 35:204–208. 2010.(In Chinese).
10	Jahan S, Khan M, Imran S and Sair M: The hepatoprotective role of Silymarin in isoniazid induced liver damage of rabbits. J Pak Med Assoc. 65:620–622. 2015.PubMed/NCBI
11	Li GJ, Sun P, Wang Q, Qian Y, Zhu K and Zhao X: Dendrobium candidum Wall. ex Lindl. attenuates CCl₄-induced hepatic damage in imprinting control region mice. Exp Ther Med. 8:1015–1021. 2014.PubMed/NCBI
12	Melgar S, Karlsson L, Rehnström E, Karlsson A, Utkovic H, Jansson L and Michaëlsson E: Validation of murine dextran sulfate sodium-induced colitis using four therapeutic agents for human inflammatory bowel disease. Int Immunopharmacol. 8:836–844. 2008. View Article : Google Scholar : PubMed/NCBI
13	Chen SS, Zhu K, Wang R and Zhao X: Preventive effect of polysaccharides from the large yellow croaker swim bladder on HCl/ethanol induced gastric injury in mice. Exp Ther Med. 8:316–322. 2014.PubMed/NCBI
14	Liu D, He H, Yin D, Que A, Tang L, Liao Z, Huang Q and He M: Mechanism of chronic dietary iron overload-induced liver damage in mice. Mol Med Rep. 7:1173–1179. 2013.PubMed/NCBI
15	Liu H, Qi X, Cao S and Li P: Protective effect of flavonoid extract from Chinese bayberry (Myrica rubra Sieb. et Zucc.) fruit on alcoholic liver oxidative injury in mice. J Nat Med. 68:521–529. 2014. View Article : Google Scholar : PubMed/NCBI
16	Poortahmasebi V, Alavian SM, Keyvani H, Norouzi M, Mahmoodi M and Jazayeri SM: Hepatic steatosis: prevalence and host/viral risk factors in Iranian patients with chronic hepatitis B infection. Asian Pac J Cancer Prev. 15:3879–3884. 2014. View Article : Google Scholar : PubMed/NCBI
17	Nyblom H, Berggren U, Balldin J and Olsson R: High AST/ALT ratio may indicate advanced alcoholic liver disease rather than heavy drinking. Alcohol Alcohol. 39:336–9. 2004. View Article : Google Scholar : PubMed/NCBI
18	Mabrouk M, El-Raziky M, Zayed N, Salama R, El-Akel W, Awad T, El Beshlawy M and Esmat G: Clinical, biochemical and pathological profiles of 5464 Egyptian chronic hepatitis C-infected patients. Hepatogastroenterology. 60:1731–1735. 2013.PubMed/NCBI
19	Dickens H, Ullrich A, Runge D, Mueller B, Olszewski U and Hamilton G: Anticancer drug cis-4-hydroxy-L-proline: Correlation of preclinical toxicology with clinical parameters of liver function. Mol Med Rep. 1:459–464. 2008.PubMed/NCBI
20	Srivastava SP, Chen NQ and Holtzman JL: The in vitro NADPH-dependent inhibition by CCl₄ of the ATP-dependent calcium uptake of hepatic microsomes from male rats. Studies on the mechanism of the inactivation of the hepatic microsomal calcium pump by the CCl₃ radical. J Biol Chem. 265:8392–8399. 1990.PubMed/NCBI
21	Liu L, Fan H, Qi P, Mei Y, Zhou L, Cai L, Lin X and Lin J: Synthesis and hepatoprotective properties of Acanthus ilicifolius alkaloid A and its derivatives. Exp Ther Med. 6:796–802. 2013.PubMed/NCBI
22	Karabulut AB, Kafkas ME, Kafkas AS, Onal Y and Kiran TR: The effect of regular exercise and massage on oxidant and antioxidant parameters. Indian J Physiol Pharmacol. 57:378–383. 2013.PubMed/NCBI
23	Yin L, Wei L, Fu R, Ding L, Guo Y, Tang L and Chen F: Antioxidant and hepatoprotective activity of Veronica ciliata Fisch. extracts against carbon tetrachloride-induced liver injury in mice. Molecules. 19:7223–7236. 2014. View Article : Google Scholar : PubMed/NCBI
24	Ranjbar A, Sharifzadeh M, Karimi J, Tavilani H, Baeeri M, Shayesteh Heidary T and Abdollahi M: Propofol attenuates toxic oxidative stress by CCl₄ in liver mitochondria and blood in rat. Iran J Pharm Res. 13:253–262. 2014.PubMed/NCBI
25	Tomizawa M, Kawanabe Y, Shinozaki F, Sato S, Motoyoshi Y, Sugiyama T, Yamamoto S and Sueishi M: Elevated levels of alanine transaminase and triglycerides within normal limits are associated with fatty liver. Exp Ther Med. 8:759–762. 2014.PubMed/NCBI
26	Song HY, Mao ZM, Yang LL, Liu T, Li DF, Zhang L, Ge YL, Zheng PY, Liu P, Zhang XQ and Ji G: Dangfei liganning capsules attenuate the susceptibility of rat nonalcoholic fatty liver to carbon tetrachloride toxicity. J Tradit Chin Med. 31:327–333. 2011. View Article : Google Scholar : PubMed/NCBI
27	Sun Y, Yang X, Lu X, Wang D and Zhao Y: Protective effects of Keemun black tea polysaccharides on acute carbon tetrachloride-caused oxidative hepatotoxicity in mice. Food Chem Toxicol. 58:184–192. 2013. View Article : Google Scholar : PubMed/NCBI
28	Schett G, Elewaut D, McInnes IB, Dayer JM and Neurath MF: How cytokine networks fuel inflammation: Toward a cytokine-based disease taxonomy. Nat Med. 19:822–824. 2013. View Article : Google Scholar : PubMed/NCBI
29	Bae UJ, Yang JD, Ka SO, Koo JH, Woo SJ, Lee YR, Yu HC, Cho BH, Zhao HY, Ryu JH, Lee SM, et al: SPA0355 attenuates ischemia/reperfusion-induced liver injury in mice. Exp Mol Med. 46:e1092014. View Article : Google Scholar : PubMed/NCBI
30	Yang Y, Yang J and Jiang Q: The protective effect of huperzine A against hepatic ischemia reperfusion injury in mice. Transplant Proc. 46:1573–1577. 2014. View Article : Google Scholar : PubMed/NCBI
31	Bodienkova GM, Alekseev R, Boklazhenko E and Kurchevenko S: Inflammation mediators in employees in chronic exposure to neurotoxicants. Int J Occup Med Environ Health. 27:619–626. 2014. View Article : Google Scholar : PubMed/NCBI
32	Malekinejad H, Rezabakhsh A, Rahmani F and Razi M: Paraquat exposure up-regulates cyclooxygenase-2 in the lungs, liver and kidneys in rats. Iran J Pharm Res. 12:887–896. 2013.PubMed/NCBI
33	Ibrahim MA, Abdel-Gaber SA, Amin EF, Ibrahim SA, Mohammed RK and Abdelrahman AM: Molecular mechanisms contributing to the protective effect of levosimendan in liver ischemia-reperfusion injury. Eur J Pharmacol. 741:64–73. 2014. View Article : Google Scholar : PubMed/NCBI
34	Dong M, Hong T, Liu S, Zhao J, Meng Y and Mu J: Hepatoprotective effect of the flavonoid fraction isolated from the flower of Inula britannica against D-Galactosamine-induced hepatic injury. Mol Med Rep. 7:1919–1923. 2013.PubMed/NCBI
35	Huang GJ, Deng JS, Huang SS, Lee CY, Hou WC, Wang SY, Sung PJ and Kuo YH: Hepatoprotective effects of eburicoic acid and dehydroeburicoic acid from Antrodia camphorata in a mouse model of acute hepatic injury. Food Chem. 141:3020–3027. 2013. View Article : Google Scholar : PubMed/NCBI