Triptolide reduces the viability of osteosarcoma cells by reducing MKP-1 and Hsp70 expression

Zhao,Lei; Jiang,Bo; Wang,Dong; Liu,Wei; Zhang,Huawu; Liu,Weisheng; Qiu,Zhen

doi:10.3892/etm.2016.3164

Triptolide reduces the viability of osteosarcoma cells by reducing MKP-1 and Hsp70 expression

Authors:
- Lei Zhao
- Bo Jiang
- Dong Wang
- Wei Liu
- Huawu Zhang
- Weisheng Liu
- Zhen Qiu
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Affiliations: Department of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Institute of Radiation Medicine, Shandong Academy of Medical Science, Jinan, Shandong 250062, P.R. China
Published online on: March 15, 2016 https://doi.org/10.3892/etm.2016.3164
Pages: 2005-2010

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Abstract

Osteosarcoma is the most common type of malignant bone tumor found in adolescents and young adults. The aim of the present study was to determine whether triptolide, a diterpene epoxide extracted from the Tripterygium plant, was able effectively decrease the viability of osteosarcoma cells. The underlying molecular mechanisms are also investigated. The human osteosarcoma cell lines U‑2 OS and MG‑63 were used in this study. The U‑2 OS and MG‑63 cells were treated with 0, 5, 10, 25 or 50 nM triptolide. Cells treated with dimethyl sulfoxide only were used as the no drug treatment control. A commercial MTT kit was used to determine the effects of triptolide on cells. Mitogen‑activated protein kinase phosphatase‑1 (MKP‑1) is frequently overexpressed in tumor tissues, possibly related to the failure of a number of chemotherapeutics. Heat shock protein 70 (Hsp70) is a chaperone molecule that is able to increase drug resistance. The protein expression levels of MKP‑1 and Hsp70 were determined using western blot analysis. The results indicate that triptolide effectively reduced the viability of the osteosarcoma cells. Furthermore, triptolide was found to effectively reduce MKP‑1 expression and Hsp70 levels. Further analysis showed that triptolide reduced MKP‑1 mRNA expression in the U‑2 OS and MG‑63 cells. Triptolide reduced Hsp70 mRNA expression levels in U‑2 OS and MG‑63 cells. These results suggest that triptolide effectively decreases the viability of osteosarcoma cells. These effects may be associated with the decreased expression of MKP‑1 and Hsp70 levels. These results suggest that triptolide may be used in the treatments of osteosarcoma.

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