Epidemiology of Ureaplasma urealyticum and Mycoplasma hominis in the semen of male outpatients with reproductive disorders

Zhu,Xiaofei; Li,Min; Cao,Huiling; Yang,Xuewen; Zhang,Chunbing

doi:10.3892/etm.2016.3409

Epidemiology of Ureaplasma urealyticum and Mycoplasma hominis in the semen of male outpatients with reproductive disorders

Authors:
- Xiaofei Zhu
- Min Li
- Huiling Cao
- Xuewen Yang
- Chunbing Zhang
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Affiliations: Department of Laboratory Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, P.R. China
Published online on: May 31, 2016 https://doi.org/10.3892/etm.2016.3409
Pages: 1165-1170

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Abstract

The aim of the present study was to investigate the association between Mycoplasma infection and infertility in male outpatients among a Chinese population. Epidemiological data, including prevalence, age distribution and antibiotic resistance profile of patients with an Ureaplasma urealyticum or Mycoplasma hominis infection were collected between 2009 and 2012. Among the 7,374 individuals analyzed, 3,225 patients (43.7%) were determined to be positive for infection with U. urealyticum, M. hominis or for both Mycoplasmas. Among the positive cultures, U. urealyticum was detected most frequently, while M. hominis was rarely found. The age range of 25‑34 years was the preferred period for the positive detection. Tetracyclines and josamycin were the most effective agents against both genital Mycoplasmas, including in the case of co‑infection. Macrolides (erythromycin, roxithromycin, azithromycin, clarithromycin except for josamycin) were effective against the majority of U. urealyticum clinical isolates, but were naturally resisted by M. hominis in this study. Fluoroquinolones had the lowest activity against U. urealyticum, particularly in cases of M. hominis co‑infection. Furthermore, fluoroquinolones showed a similar pattern of drug resistance against M. hominis to that of U. urealyticum. Antibiotic resistance did not vary significantly over the test period. Notably, an elevated multi‑drug resistance rate was observed in patients co‑infected with both Mycoplasmas. In light of the epidemiological characteristics of genital Mycoplasmas in male infertility patients, the present results may aid Chinese clinicians to implement rational drug usage and avoid the overuse of antibiotics.

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1	Horner P, Blee K and Adams E: Time to manage Mycoplasma genitalium as an STI: But not with azithromycin 1 g! Curr Opin Infect Dis. 27:68–74. 2014. View Article : Google Scholar : PubMed/NCBI
2	Citti C and Blanchard A: Mycoplasmas and their host: Emerging and re-emerging minimal pathogens. Trends Microbiol. 21:196–203. 2013. View Article : Google Scholar : PubMed/NCBI
3	Waites KB, Katz B and Schelonka RL: Mycoplasmas and ureaplasmas as neonatal pathogens. Clin Microbiol Rev. 18:757–789. 2005. View Article : Google Scholar : PubMed/NCBI
4	McCormack WM: Susceptibility of mycoplasmas to antimicrobial agents: Clinical implications. Clin Infect Dis. 17(Suppl 1): S200–S201. 1993. View Article : Google Scholar : PubMed/NCBI
5	Furneri PM, Rappazzo G, Musumarra MP, Di Pietro P, Catania LS and Roccasalva LS: Two new point mutations at A2062 associated with resistance to 16-membered macrolide antibiotics in mutant strains of Mycoplasma hominis. Antimicrob Agents Chemother. 45:2958–2960. 2001. View Article : Google Scholar : PubMed/NCBI
6	Dégrange S, Renaudin H, Charron A, Bébéar C and Bébéar CM: Tetracycline resistance in Ureaplasma spp. and Mycoplasma hominis: Prevalence in Bordeaux, France, from 1999 to 2002 and description of two tet (M)-positive isolates of M. hominis susceptible to tetracyclines. Antimicrob Agents Chemother. 52:742–744. 2008. View Article : Google Scholar : PubMed/NCBI
7	Pereyre S, Renaudin H, Charron A, Bébéar C and Bébéar CM: Emergence of a 23S rRNA mutation in Mycoplasma hominis associated with a loss of the intrinsic resistance to erythromycin and azithromycin. J Antimicrob Chemother. 57:753–756. 2006. View Article : Google Scholar : PubMed/NCBI
8	Mardassi BB, Aissani N, Moalla I, Dhahri D, Dridi A and Mlik B: Evidence for the predominance of a single tet(M) gene sequence type in tetracycline-resistant Ureaplasma parvum and Mycoplasma hominis isolates from Tunisian patients. J Med Microbiol. 61:1254–1261. 2012. View Article : Google Scholar : PubMed/NCBI
9	Waites KB, Crabb DM, Bing X and Duffy LB: In vitro susceptibilities to and bactericidal activities of garenoxacin (BMS-284756) and other antimicrobial agents against human Mycoplasmas and Ureaplasmas. Antimicrob Agents Chemother. 47:161–165. 2003. View Article : Google Scholar : PubMed/NCBI
10	Wang QY, Li RH, Zheng LQ and Shang XH: Prevalence and antimicrobial susceptibility of Ureaplasma urealyticum and Mycoplasma hominis in female outpatients, 2009–2013. J Microbiol Immunol Infect. 2014.[Epub ahead of print].
11	De Francesco MA, Caracciolo S, Bonfanti C and Manca N: Incidence and antibiotic susceptibility of Mycoplasma hominis and Ureaplasma urealyticum isolated in Brescia, Italy, over 7 years. J Infect Chemother. 19:621–627. 2013. View Article : Google Scholar : PubMed/NCBI
12	Clinical and Laboratory Standards Institute (CLSI): Methods for antimicrobial susceptibility testing for human mycoplasmas; Approved guideline. CLSI Document M43-A. CLSI. (Wayne, PA). 2–5. 2011.
13	Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, Harbarth S, Hindler JF, Kahlmeter G, Olsson-Liljequist B, et al: Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 18:268–281. 2012. View Article : Google Scholar : PubMed/NCBI
14	Wang YX, You L, Zeng Q, Sun Y, Huang YH, Wang C, Wang P, Cao WC, Yang P, Li YF and Lu WQ: Phthalate exposure and human semen quality: Results from an infertility clinic in China. Environ Res. 142:1–9. 2015. View Article : Google Scholar : PubMed/NCBI
15	Hu W, Chen M, Wu W, Lu J, Zhao D, Pan F, Lu C, Xia Y, Hu L, Chen D, et al: Gene-gene and gene-environment interactions on risk of male infertility: Focus on the metabolites. Environ Int. 91:188–195. 2016. View Article : Google Scholar : PubMed/NCBI
16	Gimenes F, Souza RP, Bento JC, Teixeira JJ, Maria-Engler SS, Bonini MG and Consolaro ME: Male infertility: A public health issue caused by sexually transmitted pathogens. Nat Rev Urol. 11:672–687. 2014. View Article : Google Scholar : PubMed/NCBI
17	Crăcea E, Măicănescu-Georgescu M, Constantinescu S and Botez D: Genital Mycoplasmas and Chlamydiae in male infertility. Arch Roum Pathol Exp Microbiol. 41:219–224. 1982.PubMed/NCBI
18	de Barbeyrac B, Bernet-Poggi C, Fébrer F, Renaudin H, Dupon M and Bébéar C: Detection of Mycoplasma pneumoniae and Mycoplasma genitalium in clinical samples by polymerase chain reaction. Clin Infect Dis. 17(Suppl 1): S83–S89. 1993. View Article : Google Scholar : PubMed/NCBI
19	Oriel JD: Role of genital mycoplasmas in nongonococcal urethritis and prostatitis. Sex Transm Dis. 10(Suppl 4): S263–S270. 1983.
20	Al-Sweih NA, Al-Fadli AH, Omu AE and Rotimi VO: Prevalence of Chlamydia trachomatis, Mycoplasma hominis, Mycoplasma genitalium and Ureaplasma urealyticum infections and seminal quality in infertile and fertile men in Kuwait. J Androl. 33:1323–1329. 2012. View Article : Google Scholar : PubMed/NCBI
21	Gdoura R, Kchaou W, Chaari C, Znazen A, Keskes L, Rebai T and Hammami A: Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis and Mycoplasma genitalium infections and semen quality of infertile men. BMC Infect Dis. 7:1292007. View Article : Google Scholar : PubMed/NCBI
22	Filipiak E, Marchlewska K, Oszukowska E, Walczak-Jedrzejowska R, Swierczynska-Cieplucha A, Kula K and Slowikowska-Hilczer J: Presence of aerobic micro-organisms and their influence on basic semen parameters in infertile men. Andrologia. 47:826–831. 2015. View Article : Google Scholar : PubMed/NCBI
23	Lee JS, Kim KT, Lee HS, Yang KM, Seo JT and Choe JH: Concordance of Ureaplasma urealyticum and Mycoplasma hominis in infertile couples: Impact on semen parameters. Urology. 81:1219–1224. 2013. View Article : Google Scholar : PubMed/NCBI
24	Abusarah EA, Awwad ZM, Charvalos E and Shehabi AA: Molecular detection of potential sexually transmitted pathogens in semen and urine specimens of infertile and fertile males. Diagn Microbiol Infect Dis. 77:283–286. 2013. View Article : Google Scholar : PubMed/NCBI
25	Salmeri M, Valenti D, La Vignera S, Bellanca S, Morello A, Toscano MA, Mastrojeni S and Calogero AE: Prevalence of Ureaplasma urealyticum and Mycoplasma hominis infection in unselected infertile men. J Chemother. 24:81–86. 2012. View Article : Google Scholar : PubMed/NCBI
26	Taylor-Robinson D and Bébéar C: Antibiotic susceptibilities of Mycoplasmas and treatment of mycoplasmal infections. J Antimicrob Chemother. 40:622–630. 1997. View Article : Google Scholar : PubMed/NCBI