Increased soluble and membrane-bound PD-L1 contributes to immune regulation and disease progression in patients with tuberculous pleural effusion

  • Authors:
    • Xue Pan
    • Anyuan Zhong
    • Yufei Xing
    • Minhua Shi
    • Bin Qian
    • Tong Zhou
    • Yongjing Chen
    • Xueguang Zhang
  • View Affiliations

  • Published online on: August 23, 2016     https://doi.org/10.3892/etm.2016.3611
  • Pages: 2161-2168
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Abstract

Soluble and membrane-bound programmed death ligand-1 (sPD-L1 and mPD-L1, respectively) have been demonstrated to participate in the immune suppression of non‑small cell lung cancer. However, the contribution of sPD‑L1 and mPD‑L1 to immune regulation and disease progression in patients with pleural effusions remains unknown. The present study evaluated the levels of sPD‑L1 and membrane‑bound PD‑1/PD‑L1 in the peripheral blood and pleural effusions of patients with tuberculous pleural effusion (TPE), malignant pleural effusion (MPE) and non‑tuberculous non‑malignant pleural effusion (n‑TB n‑M). Furthermore, selected T lymphocytes and cluster of differentiation (CD)14+ monocytes were co‑cultured to investigate the potential effect of the PD‑1/PD‑L1 pathway in TPE. Levels of sPD‑L1 and PD‑L1 on CD14+ monocytes were increased in the TPE group, as compared with the MPE and n‑TB n‑M groups. Furthermore, sPD‑L1 levels and the expression levels of PD‑L1 on CD14+ monocytes were demonstrated to be positively correlated with interferon (IFN)‑γ concentration in pleural effusions. Therefore, IFN‑γ may increase the expression of PD‑L1 on CD14+ monocytes in vitro. Cell counting kit‑8 analysis demonstrated that anti‑PD‑L1 antibody was able to partially reverse the proliferation of T lymphocytes in the co‑culture system. The results of the present study indicated that sPD‑L1 or mPD‑L1 are associated with the immune regulation and disease progression of TPE, and may serve as possible biomarkers of TPE. Furthermore, sPD‑L1 and the PD‑1/PD‑L1 pathway of TPE may be associated with the Th1 immune response; therefore, an anti‑PD‑1/PD‑L1 pathway suggests a potential immune therapy strategy for the treatment of TPE.
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October-2016
Volume 12 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Pan X, Zhong A, Xing Y, Shi M, Qian B, Zhou T, Chen Y and Zhang X: Increased soluble and membrane-bound PD-L1 contributes to immune regulation and disease progression in patients with tuberculous pleural effusion. Exp Ther Med 12: 2161-2168, 2016.
APA
Pan, X., Zhong, A., Xing, Y., Shi, M., Qian, B., Zhou, T. ... Zhang, X. (2016). Increased soluble and membrane-bound PD-L1 contributes to immune regulation and disease progression in patients with tuberculous pleural effusion. Experimental and Therapeutic Medicine, 12, 2161-2168. https://doi.org/10.3892/etm.2016.3611
MLA
Pan, X., Zhong, A., Xing, Y., Shi, M., Qian, B., Zhou, T., Chen, Y., Zhang, X."Increased soluble and membrane-bound PD-L1 contributes to immune regulation and disease progression in patients with tuberculous pleural effusion". Experimental and Therapeutic Medicine 12.4 (2016): 2161-2168.
Chicago
Pan, X., Zhong, A., Xing, Y., Shi, M., Qian, B., Zhou, T., Chen, Y., Zhang, X."Increased soluble and membrane-bound PD-L1 contributes to immune regulation and disease progression in patients with tuberculous pleural effusion". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2161-2168. https://doi.org/10.3892/etm.2016.3611