WT1 shRNA delivery using transferrin-conjugated PEG liposomes in an in vivo model of melanoma

  • Authors:
    • Santiago Saavedra‑Alonso
    • Pablo Zapata‑Benavides
    • Ana Karina Chavez‑Escamilla
    • Edgar Manilla‑Muñoz
    • Diana Elisa Zamora‑Avila
    • Moisés Armides Franco‑Molina
    • Cristina Rodriguez‑Padilla
  • View Affiliations

  • Published online on: October 27, 2016     https://doi.org/10.3892/etm.2016.3851
  • Pages: 3778-3784
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Abstract

The global incidence of melanoma is increasing. Mortality from melanoma is influenced primarily by metastasis in advanced stages of the disease. Current treatments are largely ineffective; thus, novel gene delivery approaches that target tumor-specific markers may be useful for the treatment of melanoma. Systemic administration of encapsulated RNA‑interference plasmids targeted against tumor cells is a potential alternative therapy for cancer. Formulations of transferrin (Tf)-conjugated polyethylene glycol (PEG) liposomes loaded with short hairpin RNA (shRNA) against WT1 (Lip + RNAi + Tf), PEG liposomes loaded with shRNA against WT1 (Lip + RNAi), Tf‑conjugated PEG liposomes loaded with pEGFP‑N3 (Lip + GFP + Tf) and saline solution as negative control (untreated) were administered systemically to C57BL/6 mice implanted subcutaneously with a melanoma cell line. Tumor volume, body weight, tumor weight, survival and relative expression of WT1 were evaluated. No significant differences in net body weight were identified between groups. The tumor volume decreased from 7,871 mm3 (SD±2,087) in the untreated group to 5,981 mm3 (SD±2,099) in the Lip + RNAi + Tf group. The tumor weight was reduced, from 8.8 g (SD±0.30) in the untreated group to 5.5 g (SD±0.87) in the Lip + RNAi + Tf group. An increase of 37% in survival was also observed in the group treated with Lip + RNAi + Tf in comparison to the untreated group. Tumors treated with Lip + RNAi + Tf also showed a decrease in the mean relative expression of WT1 of 0.21 (SD±0.28) folds compared with 1.8 (SD±2.49) folds in untreated group, 1.34 (SD±0.43) folds in Lip + RNAi group and of 1.89 (SD±0.69) folds in Lip + GFP + Tf group. Systemic administration of transferrin‑conjugated PEG liposomes loaded with shRNA against WT1 reduced WT1 expression and tumor size and increased survival.
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December-2016
Volume 12 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Saavedra‑Alonso S, Zapata‑Benavides P, Chavez‑Escamilla AK, Manilla‑Muñoz E, Zamora‑Avila DE, Franco‑Molina MA and Rodriguez‑Padilla C: WT1 shRNA delivery using transferrin-conjugated PEG liposomes in an in vivo model of melanoma. Exp Ther Med 12: 3778-3784, 2016.
APA
Saavedra‑Alonso, S., Zapata‑Benavides, P., Chavez‑Escamilla, A.K., Manilla‑Muñoz, E., Zamora‑Avila, D.E., Franco‑Molina, M.A., & Rodriguez‑Padilla, C. (2016). WT1 shRNA delivery using transferrin-conjugated PEG liposomes in an in vivo model of melanoma. Experimental and Therapeutic Medicine, 12, 3778-3784. https://doi.org/10.3892/etm.2016.3851
MLA
Saavedra‑Alonso, S., Zapata‑Benavides, P., Chavez‑Escamilla, A. K., Manilla‑Muñoz, E., Zamora‑Avila, D. E., Franco‑Molina, M. A., Rodriguez‑Padilla, C."WT1 shRNA delivery using transferrin-conjugated PEG liposomes in an in vivo model of melanoma". Experimental and Therapeutic Medicine 12.6 (2016): 3778-3784.
Chicago
Saavedra‑Alonso, S., Zapata‑Benavides, P., Chavez‑Escamilla, A. K., Manilla‑Muñoz, E., Zamora‑Avila, D. E., Franco‑Molina, M. A., Rodriguez‑Padilla, C."WT1 shRNA delivery using transferrin-conjugated PEG liposomes in an in vivo model of melanoma". Experimental and Therapeutic Medicine 12, no. 6 (2016): 3778-3784. https://doi.org/10.3892/etm.2016.3851