Effect of Suo Quan Wan on the bladder function of aging rats based on the β-adrenoceptor

Xu,Yi‑Fei; Liang,Zhi‑Jian; Kuang,Zhao‑Jin; Chen,Jie‑Jun; Wu,Jun; Lu,Xiang‑E; Jiang,Wei‑Wen; Fan,Ping‑Long; Tang,Li‑Yao; Li,Yi‑Tao; Huang,Ping; Cao,Hong‑Ying

doi:10.3892/etm.2017.4394

Effect of Suo Quan Wan on the bladder function of aging rats based on the β-adrenoceptor

Authors:
- Yi‑Fei Xu
- Zhi‑Jian Liang
- Zhao‑Jin Kuang
- Jie‑Jun Chen
- Jun Wu
- Xiang‑E Lu
- Wei‑Wen Jiang
- Ping‑Long Fan
- Li‑Yao Tang
- Yi‑Tao Li
- Ping Huang
- Hong‑Ying Cao
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Affiliations: Department of Chinese Medicine Pharmacology, School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China, Center for Drug Non‑Clinical Evaluation and Research, Guangzhou General Pharmaceutical Research Institute, Guangzhou, Guangdong 510240, P.R. China, Department of Chinese Medicine, School of Chinese Medicine, Shandong College of Traditional Chinese Medicine, Yantai, Shandong 264199, P.R. China, Department of Traditional Chinese Medicine, Institute of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China
Published online on: April 27, 2017 https://doi.org/10.3892/etm.2017.4394
Pages: 3424-3432
Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Suo Quan Wan (SQW) has been used to treat lower urinary tract symptoms (LUTS) in elderly patients for hundreds of years in China. β‑adrenoceptors (β‑ARs), particularly β3‑adrenoceptor (β3‑AR), was reported to be important in the bladder dysfunction of the elderly. The present study was conducted to explore the effect of β‑AR, and particularly the β3‑adrenoceptor, in aging rat bladder function in vitro and to test the therapeutic effect of SQW on LUTS in an aging rat model based on the β3‑adrenoceptor. Briefly, the bladder detrusor muscles of young (age, 3 months) and aging (age, 15 months) female rats were separated. A β‑AR non‑selective agonist, isoprenaline (ISO), subtype β3‑AR agonist (BRL37344A) and β3‑AR antagonist (SR59230A) were used to define the tension change of detrusor muscles between young and aging rats in vitro. For blank controls, 12 young rats were marked, and 48 aging female rats were randomly divided into four groups as follows: Model, SQW high, SQW middle and SQW low. Following oral administration of SQW for 6 weeks in aging rats, urodynamic and bladder detrusor tests were used to evaluate the therapeutic effect of SQW. The expression of β3‑AR mRNA was investigated using reverse transcription‑quantitative polymerase chain reaction. Using ISO and BRL37344A in vitro, maximum relaxation (Emax), intrinsic activity (IA), and log (50% effective concentration) (PD2) were significantly decreased in aging rats compared with that in young rats (P<0.05). Significant changes were also observed in the β3‑AR antagonist experiment, which blocked ISO‑induced relaxation, with significant decreases observed in Emax, IA and PD2, and a significant increase observed in PA2 for the aging rats compared with the young controls (P<0.05). SQW was demonstrated to enhance bladder control, storage and contraction ability. Furthermore, SQW was able to increase the sensitivity and expression of β3‑AR in an aging rat. In conclusion, the decrease in β3‑AR sensitivity in aging rats and the expression resulted in bladder detrusor dysfunction. In addition, the therapeutic effect of SQW against LUTS relies on the former's effect on the urethral sphincter, bladder detrusor and β3‑AR.