Open Access

Cytotoxic T lymphocytes promote cytarabine-induced acute myeloid leukemia cell apoptosis via inhibiting Bcl-2 expression

  • Authors:
    • Rui Deng
    • Fang‑Yi Fan
    • Hai Yi
    • Li Fu
    • Yan Zeng
    • Yi Wang
    • Xiao‑Juan Miao
    • Yan‑Rong Shuai
    • Guang‑Cui He
    • Yi Su
  • View Affiliations

  • Published online on: June 16, 2017     https://doi.org/10.3892/etm.2017.4620
0

Abstract

Acute myeloid leukemia (AML) remains difficult to cure due to its drug tolerance and refractoriness. Immunotherapy is a growing area of cancer research, which has been applied for the treatment of numerous types of cancer, including leukemia. The present study generated AML cell‑specific cytotoxic T lymphocytes (CTLs) in vitro and investigated the effect of combining CTL treatment with one of the most commonly used drugs for the treatment of hematological malignancies, cytarabine, on AML cell apoptosis. Firstly, it was observed that monocyte‑depleted peripheral blood lymphocytes from healthy donors could be used to generate large numbers of CD3+CD8+ CTLs through immune stimulation. These CD3+CD8+ CTLs could effectively recognize and induce the apoptosis of human Kasumi‑3 AML cells. In addition, cytarabine‑induced AML cell apoptosis was enhanced by CTL treatment. Western blotting revealed that Bcl‑2 expression was downregulated in AML cells following cytarabine and CTL treatment, indicating that the synergistic effect of this treatment on AML cell apoptosis is due to the downregulation of Bcl‑2. These results highlight the potential application of CTL immunotherapy for the treatment of AML. Further studies optimizing the specificity and potency of CTLs, and identifying favorable combinations with other chemotherapeutic drug are required.

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Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Deng, R., Fan, F., Yi, H., Fu, L., Zeng, Y., Wang, Y. ... Su, Y. (1899). Cytotoxic T lymphocytes promote cytarabine-induced acute myeloid leukemia cell apoptosis via inhibiting Bcl-2 expression. Experimental and Therapeutic Medicine, 0, 0-0. https://doi.org/10.3892/etm.2017.4620
MLA
Deng, R., Fan, F., Yi, H., Fu, L., Zeng, Y., Wang, Y., Miao, X., Shuai, Y., He, G., Su, Y."Cytotoxic T lymphocytes promote cytarabine-induced acute myeloid leukemia cell apoptosis via inhibiting Bcl-2 expression". Experimental and Therapeutic Medicine 0.0 (1899): 0-0.
Chicago
Deng, R., Fan, F., Yi, H., Fu, L., Zeng, Y., Wang, Y., Miao, X., Shuai, Y., He, G., Su, Y."Cytotoxic T lymphocytes promote cytarabine-induced acute myeloid leukemia cell apoptosis via inhibiting Bcl-2 expression". Experimental and Therapeutic Medicine 0, no. 0 (1899): 0-0. https://doi.org/10.3892/etm.2017.4620