Open Access

Multicomponent quantification of Astragalus residue fermentation liquor using ion chromatography-integrated pulsed amperometric detection

  • Authors:
    • Ying Zhang
    • Jiarong Wu
    • Quanhui Ni
    • Hong Dong
  • View Affiliations

  • Published online on: June 26, 2017     https://doi.org/10.3892/etm.2017.4673
  • Pages: 1526-1530
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Chinese medicine residues contain abundant cellulose and hemicellulose, which are potential renewable carbon sources for ethanol production. The aim of the present study was to develop a rapid and reliable method to evaluate the cellulose and hemicellulose utilization in Chinese medicine residues. In the present study, key hydrolysates (arabinose, galactose, glucose, xylose, and cellobiose) of the cellulose and hemicellulose in fermentation liquor of Astragalus residues were simultaneously quantified by ion chromatography using an integrated pulsed amperometric detector (IPAD). HPLC analysis was performed on a Dionex ICS-2500 equipped with GP50 gradient pump and ED50 IPAD. The working and reference electrodes were gold electrode and Ag/AgCl electrode, respectively. Separation was achieved on serial no. 002627 Dionex Analytical column (2x250 mm). Sodium hydroxide of 250 mM and water were used as the mobile phase with a flow rate of 0.2 ml/min. The temperature of column was kept at 30˚C. This method was validated for accuracy and precision. The regression equation revealed a good linear relationship (R2=0.9959-0.9984) within the test ranges. The limits of detection and quantification for five standard analytes (arabinose, galactose, glucose, xylose and cellobiose) were in the range of 0.067‑0.091 and 0.08-0.23 mg/l, respectively. The method showed good reproducibility for the quantification of five analytes in fermentation liquor of Astragalus residue with intra-and inter-day variations less than 3.843%.
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August-2017
Volume 14 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhang Y, Wu J, Ni Q and Dong H: Multicomponent quantification of Astragalus residue fermentation liquor using ion chromatography-integrated pulsed amperometric detection. Exp Ther Med 14: 1526-1530, 2017
APA
Zhang, Y., Wu, J., Ni, Q., & Dong, H. (2017). Multicomponent quantification of Astragalus residue fermentation liquor using ion chromatography-integrated pulsed amperometric detection. Experimental and Therapeutic Medicine, 14, 1526-1530. https://doi.org/10.3892/etm.2017.4673
MLA
Zhang, Y., Wu, J., Ni, Q., Dong, H."Multicomponent quantification of Astragalus residue fermentation liquor using ion chromatography-integrated pulsed amperometric detection". Experimental and Therapeutic Medicine 14.2 (2017): 1526-1530.
Chicago
Zhang, Y., Wu, J., Ni, Q., Dong, H."Multicomponent quantification of Astragalus residue fermentation liquor using ion chromatography-integrated pulsed amperometric detection". Experimental and Therapeutic Medicine 14, no. 2 (2017): 1526-1530. https://doi.org/10.3892/etm.2017.4673