Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection

Kong,Fanyun; Feng,Bo; Zhang,Henghui; Rao,Huiying; Wang,Jianghua; Cong,Xu; Wei,Lai

doi:10.3892/etm.2017.4682

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection

Authors:
- Fanyun Kong
- Bo Feng
- Henghui Zhang
- Huiying Rao
- Jianghua Wang
- Xu Cong
- Lai Wei
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Affiliations: Beijing Key Laboratory for Hepatitis C and Immunotherapy for Liver Disease, Peking University People's Hospital, Peking University Hepatology Institute, Beijing 100044, P.R. China
Published online on: June 27, 2017 https://doi.org/10.3892/etm.2017.4682
Pages: 1846-1852

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Abstract

Hepatitis C virus (HCV) infection is associated with B cell abnormality; however the phenotypic profiles of immunoglobulin (Ig)M+B cell subsets in patients with HCV infection remain unclear. In the current study, the effect of HCV infection on IgM+B cell subsets was evaluated. The percentages, as well as the differentiation and activation features of peripheral IgM+B naive subsets [cluster of differentiation (CD)27‑IgM+B cells] and IgM+B memory subsets (CD27+IgM+B cells) were assessed using flow cytometry in 27 patients with chronic hepatitis C (CHC) and 20 healthy controls (HCs). The frequency of CD27+IgM+B memory subsets detected in patients with CHC was significantly higher than that in HCs (P<0.05). Although the frequency of CD27‑IgM+B naive subsets was similar in both groups, there was a significantly higher proportion of CD5+B cells detected in the CD27‑IgM+B subsets of patients with CHC compared with HCs (P<0.05). Among CD27‑IgM+B subsets, abnormal differentiation was associated with HCV infection, with significantly increased percentages of IgD+B cells and CD38+B cells in patients with CHC compared with HCs (P<0.05). In CD27+IgM+B memory subsets, the abnormality of cell differentiation was associated with a significantly increased percentage of CD38+B cells in patients with CHC compared with HCs (P<0.05). In addition, the percentage of activated CD27+IgM+B subsets in patients with CHC were significantly higher than those observed in HCs (P<0.05). The number of CD27‑IgD+IgM+B, CD27‑CD38+IgM+B and CD27+CD38+IgM+B cells were negatively correlated with HCV RNA in patients with CHC. These results suggest that HCV infection contributes to abnormalities in the percentage, differentiation and activation of IgM+B cell subsets and may disrupt the immune response mediated by IgM+B cells.

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