MicroRNA‑33b inhibits cell proliferation and glycolysis by targeting hypoxia‑inducible factor‑1α in malignant melanoma Retraction in /10.3892/etm.2021.10729

Zhao,Yue; Wu,Cuiling; Li,Lina

doi:10.3892/etm.2017.4702

MicroRNA‑33b inhibits cell proliferation and glycolysis by targeting hypoxia‑inducible factor‑1α in malignant melanoma

Retraction in: /10.3892/etm.2021.10729

Authors:
- Yue Zhao
- Cuiling Wu
- Lina Li
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Affiliations: Department of Dermatology, Heping Hospital of Changzhi Medical College, Changzhi, Shanxi 046000, P.R. China, Department of Biochemistry, Changzhi Medical College, Changzhi, Shanxi 046000, P.R. China, Department of Pathology, Changzhi Medical College, Changzhi, Shanxi 046000, P.R. China
Published online on: June 28, 2017 https://doi.org/10.3892/etm.2017.4702
Pages: 1299-1306
Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Malignant melanoma (MM) is the most aggressive type of skin cancer. MicroRNA (miR) has been implicated in the development and progression of MM; however, their underlying mechanism of action remains largely unknown. The present study aimed to investigate the role of miR‑33b in MM. Reverse transcription‑quantitative polymerase chain reaction data indicated that the expression of miR‑33b was significantly reduced (P<0.01) in MM cell lines, including WM35, WM451 and SK‑MEL‑1, when compared with human melanocyte cells. Subsequently, WM451 and SK‑MEL‑1 cells were transfected with an miR‑33b mimic or inhibitor. MTT assay data demonstrated that the viability of MM cells markedly decreased following miR‑33b overexpression; however, viability was markedly upregulated following miR‑33b knockdown. Additionally, the glycolysis level was examined. Results demonstrated that glucose consumption and lactic acid production were significantly downregulated (P<0.01) after miR‑33b upregulation, whereas these levels significantly increased in MM cells transfected with miR‑33b inhibitor (P<0.01), suggesting that miR‑33b negatively mediates the glycolysis level in MM cells. Bioinformatics indicated that hypoxia‑inducible factor (HIF)‑1α was a putative target gene of miR‑33b, and this was confirmed by a luciferase reporter assay, which demonstrated that miR‑33b was able to directly bind to the 3' untranslated region of HIF‑1α mRNA. Furthermore, the expression of HIF‑1α was negatively regulated by miR‑33b at the post‑transcriptional level in MM cells. Overexpression of HIF‑1α reversed the inhibitory effect of miR‑33b on the proliferation and glycolysis in MM cells. Finally, the results of the present study demonstrated that hexokinase 2 and lactate dehydrogenase‑A may be involved in miR‑33b/HIF‑1α mediated glycolysis in MM cells. In conclusion, these results suggest that miR‑33b inhibits cell proliferation and glycolysis by targeting HIF‑1α in MM.

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