Open Access

Erythrocyte sedimentation rate and fibrinogen concentration of whole blood influences the cellular composition of platelet‑rich plasma obtained from centrifugation methods

  • Authors:
    • Wenjing Yin
    • Zhengliang Xu
    • Jiagen Sheng
    • Xuetao Xie
    • Changqing Zhang
  • View Affiliations

  • Published online on: July 9, 2017     https://doi.org/10.3892/etm.2017.4724
  • Pages: 1909-1918
  • Copyright: © Yin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Erythrocyte sedimentation rate (ESR), which reflects the sedimentation rate of platelets, leukocytes and erythrocytes in response to centrifugal force, may influence the cellular composition of platelet‑rich plasma (PRP) obtained via centrifugation methods. However, no relevant studies have substantiated this. In the present study, blood was collected from 40 healthy volunteers and used to prepare PRP with two plasma‑based preparation systems [YinPRP and Plasma Rich in Growth Factor (PRGF) systems] and two buffy coat‑based systems (RegenPRP and WEGOPRP systems) in a single‑donor model. Volumes of PRP and platelet‑poor plasma (PPP) that were removed in the preparation process were recorded. Analyses of ESR, haematocrit, C‑reaction protein, coagulation, serum glucose and serum lipid of the whole blood used for PRP preparation were performed to evaluate the levels of ESR and the factors known to influence it. Whole blood analysis was performed to evaluate the cellular composition of PRP. Results demonstrated that there were marked positive correlations between the ESR of the whole blood used for PRP preparation and PPP removal efficiencies, platelet concentrations, platelet capture efficiencies and platelet enrichment factors of PRP formulations obtained from plasma‑based systems, and PRP yield efficiency of RegenPRP and PPP removal efficiency of WEGOPRP. Furthermore, there were marked negative correlations between ESR and concentrations and enrichment factors of platelets, leukocytes and erythrocytes of RegenPRP. Fibrinogen concentration of the whole blood, which had a marked positive correlation with ESR, also influenced the cellular composition of PRP. These findings may increase the understanding of PRP preparation and provide substantial evidence for the individualised optimisation of PRP preparation systems used in clinical practice.

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September-2017
Volume 14 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Yin W, Xu Z, Sheng J, Xie X and Zhang C: Erythrocyte sedimentation rate and fibrinogen concentration of whole blood influences the cellular composition of platelet‑rich plasma obtained from centrifugation methods. Exp Ther Med 14: 1909-1918, 2017.
APA
Yin, W., Xu, Z., Sheng, J., Xie, X., & Zhang, C. (2017). Erythrocyte sedimentation rate and fibrinogen concentration of whole blood influences the cellular composition of platelet‑rich plasma obtained from centrifugation methods. Experimental and Therapeutic Medicine, 14, 1909-1918. https://doi.org/10.3892/etm.2017.4724
MLA
Yin, W., Xu, Z., Sheng, J., Xie, X., Zhang, C."Erythrocyte sedimentation rate and fibrinogen concentration of whole blood influences the cellular composition of platelet‑rich plasma obtained from centrifugation methods". Experimental and Therapeutic Medicine 14.3 (2017): 1909-1918.
Chicago
Yin, W., Xu, Z., Sheng, J., Xie, X., Zhang, C."Erythrocyte sedimentation rate and fibrinogen concentration of whole blood influences the cellular composition of platelet‑rich plasma obtained from centrifugation methods". Experimental and Therapeutic Medicine 14, no. 3 (2017): 1909-1918. https://doi.org/10.3892/etm.2017.4724