Application of silk fibroin/chitosan/nano‑hydroxyapatite composite scaffold in the repair of rabbit radial bone defect

Ye,Peng; Yu,Bin; Deng,Jiang; She,Rong‑Feng; Huang,Wen‑Liang

doi:10.3892/etm.2017.5231

Application of silk fibroin/chitosan/nano‑hydroxyapatite composite scaffold in the repair of rabbit radial bone defect

Authors:
- Peng Ye
- Bin Yu
- Jiang Deng
- Rong‑Feng She
- Wen‑Liang Huang
View Affiliations

Affiliations: Department of Orthopaedics, Nanfang Hospital, First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Orthopaedic Trauma, The Southern Hospital Affiliated to Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Orthopaedics, The Third Hospital Affiliated to Zunyi Medical College, Zunyi, Guizhou 563000, P.R. China, Department of Orthopaedics, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550002, P.R. China, Department of Orthopaedics, The Third Hospital Affiliated to Zunyi Medical College, Guizhou, Zunyi 563000, P.R. China
Published online on: September 29, 2017 https://doi.org/10.3892/etm.2017.5231
Pages: 5547-5553

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Silk fibroin (SF), chitosan (CS) and nano‑hydroxyapatite (nHA) possess excellent biocompatibility, thus, these were used to construct a SF/CS/nHA composite scaffold. Previously published results identified that this material exhibited satisfactory physical and chemical properties, and therefore qualified as a repair material in bone tissue engineering. The aim of the present study was to investigate the capacity and mechanism of this composite scaffold in repairing bone defects. In total, 45 New Zealand white rabbits were used to model defect in the right radial bone. A radial bone defect was induced, and rabbits were divided into the following treatment groups (n=15 in each): Group A, in which the SF/CS/nHA scaffold was implanted; group B, in which the SF/CS scaffold was implanted; and group C, in which rabbits did not receive subsequent treatment. X‑ray scanning, specimen observation and histopathological examination were implemented at 1, 2, 3 and 4 months after modeling, in order to evaluate the osteogenic capacity and mechanism. At 1 month after modeling, the bone density shadow in the X‑ray scan was darker in group A as compared with that in group B. Observation of the pathological specimens indicated that normal bone tissues partially replaced the scaffold. At 2 months, the bone density shadow of group A was similar to normal bone tissues, and normal tissue began to replace the scaffold. At 3‑4 months after modeling, the X‑ray scan and histopathological observation indicated that the normal bone tissues completely replaced the scaffold in group A, with an unobstructed marrow cavity. However, the bone mass of group B was lower in comparison with that of group A. The bone defect induced in group C was filled with fibrous connective tissues. Therefore, it was concluded that the SF/CS/nHA composite scaffold may be a promising material for bone tissue engineering.

View Figures

View References

1	Xin Lei and SU Jia-can: Current status and prospect of artificial bone repair materials. J Traumatic Surg. 3:254–257. 2011.
2	Puppi D, Chiellini F, Piras AM and Chiellini E: Polymeric materials for bone and cartilage repair. Progress in Polymer Science. 35:403–440. 2010. View Article : Google Scholar
3	Pan Z and Ding JD: Poly (lactide-co-glycolide) porous scaffolds for tissue engineering and regenerative medicine. Interface focus. 2:366–377. 2012. View Article : Google Scholar : PubMed/NCBI
4	Park SH, Park DS and Shin JW, Kang YG, Kim HK, Yoon TR and Shin JW: Scaffolds for bone tissue engineering fabricated from two different materials by the rapid prototyping technique: PCL versus PLGA. J Mater Sci Mater Med. 23:2671–2678. 2012. View Article : Google Scholar : PubMed/NCBI
5	Wang Z, Li M, Yu B, Cao L, Yang Q and Su J: Nanocalcium-deficient hydroxyapatite-poly (e-caprolactone)-polyethylene glycol-poly (e-caprolactone) composite scaffolds. Int J Nanomedicine. 7:3123–3131. 2012.PubMed/NCBI
6	De Santis R, Gloria A, Russo T, D'Amora U, Zeppetelli S, Dionigi C, Sytcheva A, Herrmannsdörfer T, Dediu V and Ambrosio L: A basic approach toward the development of nanocomposite magnetic scaffolds for advanced bonetissue engineering. J Appl Polym Sci. 122:3599–3605. 2011. View Article : Google Scholar
7	Budiraharjo R, Neoh KG and Kang ET: Hydroxyapatite-coated carboxymethyl chitosan scaffolds for promoting osteoblast and stem cell differentiation. J Colloid Interface Sci. 366:224–232. 2012. View Article : Google Scholar : PubMed/NCBI
8	Alves da Silva ML, Crawford A, Mundy JM, Correlo VM, Sol P, Bhattacharya M, Hatton PV, Reis RL and Neves NM: Chitosan/polyester-based scaffolds for cartilage tissue engineering: Assessment of extracellular matrix formation. Acta Biomater. 6:1149–1157. 2010. View Article : Google Scholar : PubMed/NCBI
9	Deng J, Yu RF, Huang WL, Yuan C and Mo G: Restoration of cartilage defect with silk fibrin/chitosan biological scaffold compound by bone marrow mesenchymal stem cells in elderly rabbits. Chin J Geriatrics. 31:156–160. 2012.
10	Tiyaboonchai W, Chomchalao P, Pongcharoen S, Sutheerawattananonda M and Sobhon P: Preparation and characterization of blended Bombyx mori silk fibroin scaffolds. Fiber Polym. 12:3242011. View Article : Google Scholar
11	Murphy CM, Haugh MG and O'Brien FJ: The effect of mean pore size on cell attachment, proliferation andmigration in collagen-glycosaminoglycan scaffolds for bone tissue engineering. Biomaterials. 31:461–466. 2010. View Article : Google Scholar : PubMed/NCBI
12	Lu Q, Zhang X, Hu X and Kaplan DL: Green process to prepare silk fibroin/gelatin biomaterial scaffolds. Macromol Biosci. 10:289–298. 2010. View Article : Google Scholar : PubMed/NCBI
13	Zhang HF, Zhao CY, Fan HS, Zhang H, Pei FX and Wang GL: Histological and biomechanical study of repairing rabbit radius segmental bone defect with porous titanium. Beijing Da Xue Xue Bao. 43:724–729. 2011.(In Chinese). PubMed/NCBI
14	Rahimzadeh R, Veshkini A, Sharifi D and Hesaraki S: Value of color Doppler ultrasonography and radiography for the assessment of the cancellous bone scaffold coated with nano-hydroxyapatite in repair of radial bone in rabbit. Acta Cir Bras. 27:148–154. 2012. View Article : Google Scholar : PubMed/NCBI
15	Hao W, Dong J, Jiang M, Wu J, Cui F and Zhou D: Enhanced bone formation in large segmental radial defects by combining adipose-derived stem cells expressing bone morphogenetic protein 2 with nHA/RHLC/PLA scaffold. Int Orthop. 34:1341–1349. 2010. View Article : Google Scholar : PubMed/NCBI
16	Deng J, She R, Huang W, Dong Z, Mo G and Liu B: A silk fibroin/chitosan scaffold in combination with bone marrow-derived mesenchymal stem cells to repair cartilage defects in the rabbit knee. J Mater Sci Mater Med. 24:2037–2046. 2013. View Article : Google Scholar : PubMed/NCBI
17	Huang Y, Huang D, Zhang D, Mou Y and Liu X: Promotion effect of FTY-720OP on treatment of bone defect with allograft bone by suppressing osteoclast formation and function. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 30:426–431. 2016.(In Chinese). PubMed/NCBI
18	Salerno M, Cenni E, Fotia C, Avnet S, Granchi D, Castelli F, Micieli D, Pignatello R, Capulli M, Rucci N, et al: Bone-targeted doxorubicin-loaded nanoparticles as a tool for the treatment of skeletal metastases. Curr Cancer Drug Targets. 10:649–659. 2010. View Article : Google Scholar : PubMed/NCBI
19	Yewle JN, Puleo DA and Bachas LG: Enhanced affinity bifunctional bisphosphonates for targeted delivery of therapeutic agents to bone. Bioconjug Chem. 22:2496–2506. 2011. View Article : Google Scholar : PubMed/NCBI
20	Kelly DJ and Jacobs CR: The role of mechanical signals in regulating chondrogenesis and osteogenesis of mesenchymal stem cells. Birth Defects Res C Embryo Today. 90:75–85. 2010. View Article : Google Scholar : PubMed/NCBI
21	Jayasuriya AC and Bhat A: Fabrication and characterization of novel hybrid organic/inorganic microparticles to apply in bone regeneration. J Biomed Mater Res A. 93:1280–1288. 2010.PubMed/NCBI
22	Lee JS, Park WY, Cha JK, Jung UW, Kim CS, Lee YK and Choi SH: Periodontal tissue reaction to customized nano-hydroxyapatite block scaffold in one-wall intrabony defect: A histologic study in dogs. J Periodontal Implant Sci. 42:50–58. 2012. View Article : Google Scholar : PubMed/NCBI
23	Martínez-Vázquez FJ, Perera FH, Miranda P, Pajares A and Guiberteau F: Improving the compressive strength of bioceramic robocast scaffolds by polymer infiltration. Acta Biomater. 6:4361–4368. 2010. View Article : Google Scholar : PubMed/NCBI
24	Crouzier T, Sailhan F, Becquart P, Guillot R, Logeart-Avramoglou D and Picart C: The performance of BMP-2 loaded TCP/HAP porous ceramics with a polyelectrolyte multilayer film coating. Biomaterials. 32:7543–7554. 2011. View Article : Google Scholar : PubMed/NCBI
25	van der Pol U, Mathieu L, Zeiter S, Bourban PE, Zambelli PY, Pearce SG, Bouré LP and Pioletti DP: Augmentation of bone defect healing using a new biocomposite scaffold: An in vivo study in sheep. Acta Biomater. 6:3755–3762. 2010. View Article : Google Scholar : PubMed/NCBI
26	Liao F, Chen Y, Li Z, Wang Y, Shi B, Gong Z and Cheng X: A novel bioactive three-dimensional beta-tricalcium phosphate/chitosan scaffold for periodontal tissue engineering. J Mater Sci Mater Med. 21:489–496. 2010. View Article : Google Scholar : PubMed/NCBI
27	Zhu LY, Wang YP, Shi ZL and Zhang HM: Fabrication and properties of nanometer hydroxyapatite/calcium polyphosphate/poly (L-lactic acid) composite scaffold for bone tissue engineering. J Clin Rehabil Tissue Eng Res. 16:431–433. 2012.
28	Xiao HJ, Xue F, He ZM, Jin FQ and Shen YC: Preparation and characterization of nano-hydroxyapatite/carboxymethychitosan-sodium alginate bone cement composite material. J Clin Rehabil Tissue Eng Res. 15:7113–7117. 2011.
29	Ye R, Zhang XF, Yan HN, Pan YF, Huang NP, Lv LX and Jiang ZL: Hydroxybutyrate-hydroxyvalerate sodium meters of fiber material with bone defect repair. J Clin Rehabil Tissue Eng Res. 16:6284–6288. 2012.
30	Yang Y, Xu WY, Zhang Y, Zhang XX, Liu ZB, Qian H, Huang JP and Cui ZH: Symbol Silk fibroin/hydroxyapatite combined with bone marrow mesenchymal stem cells for construction of tissue engineered cartilage. J Clin Rehabil Tissue Eng Res. 15:5339–5342. 2011.
31	Rong Zijie, Yang Lianjun and Zhang Zanjie: The effect of nano-hydroxyapatite/collagen scaffolds incorporating ADM-PLGA microspheres in repairing the rabbits bone defects. J Pract Med. 22:3559–3562. 2014.
32	Ye Peng, Tian Ren-yuan and Ma Li-kun: Silk fibroin/chitosan/nano hydroxyapatite complicated scaffolds for bone tissue engineering. Chin J Tissue Eng Res. 29:5270–5274. 2013.
33	Ma Likun, Ye Peng and Jiang Deng: The cytotoxicity of silk fibroin/chitosan/nanohydroxyapatite bone tissue engineering scaffolds in vitro. Med J Westchina. 8:975–980. 2014.
34	Zhou H and Lee J: Nanoscale hydroxyapatite particles for bone tissue engineering. Acta Biomater. 7:2769–2781. 2011. View Article : Google Scholar : PubMed/NCBI
35	Zhang CY, Lu H, Zhuang Z, Wang XP and Fang QF: Nano-hydroxyapatite/poly (l-lactic acid) composite synthesized by a modified in situ precipitation: Preparation and properties. J Mater Sci Mater Med. 21:3077–3083. 2010. View Article : Google Scholar : PubMed/NCBI