Analysis on the association between sperm DNA fragmentation index and conventional semen parameters, blood microelements and seminal plasma ROS in male patients with infertility

Xie,Deng'e; Lu,Chen; Zhu,Ying; Zhu,Sulan; Yang,Er-Jiang; Jin,Xin

doi:10.3892/etm.2018.6115

Analysis on the association between sperm DNA fragmentation index and conventional semen parameters, blood microelements and seminal plasma ROS in male patients with infertility

Authors:
- Deng'e Xie
- Chen Lu
- Ying Zhu
- Sulan Zhu
- Er-Jiang Yang
- Xin Jin
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Affiliations: Department of Urology, Shiyan Taihe Hospital, Affiliated Hospital to Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Department of Laboratory, Affiliated Hospital of Gannan Medical College,Ganzhou, Jiangxi 341000, P.R. China, Department of Blood Transfusion, Affiliated Hospital of Gannan Medical College, Ganzhou, Jiangxi 341000, P.R. China, Department of Laboratory, The Second Affiliated Hospital of Gannan Medical College, Ganzhou, Jiangxi 341600, P.R. China, Department of Urology, Shiyan Taihe Hospital, Affiliated Hospital to Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, The Reproductive Center, Huaian First People's Hospital, Nanjing Medical University, Huaian, Jiangsu 223001, P.R. China
Published online on: May 2, 2018 https://doi.org/10.3892/etm.2018.6115
Pages: 5173-5176
Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

We conducted this study to analyze the sperm DNA fragmentation index, conventional semen parameters, blood microelements and seminal plasma reactive oxygen species (ROS) in patients with male infertility to determine the association between each of the above male physiological parameters and DNA fragmentation index and infertility. Eighty cases of infertile males and 20 cases of normal males with children were divided into the infertility and control groups, respectively. Sperm DNA fragmentation index, conventional semen parameters, serum microelement content and seminal plasma ROS levels were detected, and the existing correlation between sperm DNA fragmentation index and the various physiological parameters were studied. The sperm DNA fragmentation index had no correlation with conventional sperm parameters. Our results demonstrated that zinc, lead and magnesium ions in the serum microelements were correlated with sperm DNA fragmentation (p<0.05). Upon an increase in zinc and lead serum concentration, there was a subsequent increase in sperm DNA fragmentation (p=0.008). Furthermore, when magnesium ion increased, it also caused an increase in sperm DNA fragmentation (p<0.05). The seminal plasma ROS of infertile males was higher than that of males with children (p<0.05). Our results suggest that sperm DNA fragmentation index is closely associated with the infertility rate and microelements of serum and seminal plasma ROS can impact the formation of sperm DNA fragmentation. Therefore, the sperm DNA fragmentation index can serve as an important parameter to assess male infertility.

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