Open Access

Gambogic acid regulates the migration and invasion of colorectal cancer via microRNA‑21‑mediated activation of phosphatase and tensin homolog

  • Authors:
    • Guangyi Gao
    • Yinzhu Bian
    • Hanqing Qian
    • Mi Yang
    • Jing Hu
    • Li Li
    • Lixia Yu
    • Baorui Liu
    • Xiaoping Qian
  • View Affiliations

  • Published online on: July 6, 2018     https://doi.org/10.3892/etm.2018.6421
  • Pages: 1758-1765
  • Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 6110 (Spandidos Publications: 5597 | PMC Statistics: 513 )
Total PDF Downloads: 1812 (Spandidos Publications: 1668 | PMC Statistics: 144 )


Abstract

Gambogic acid (GA) has been reported to inhibit cancer cell proliferation and migration and enhance apoptosis. Several signaling pathways were identified to be involved in GA function, including PI3K/Akt, caspase‑3 apoptosis and TNF‑α/NF‑κB. However, to the best of our knowledge, the association between miRNA and GA has not been explored. The present study initially demonstrated that GA could inhibit HT‑29 cancer cell proliferation using an MTT assay. In addition, a Transwell assay and a wound‑healing assay respectively indicated that GA inhibited HT‑29 cancer cell invasion and migration, which was also confirmed by the increased MMP‑9 protein expression. Furthermore, GA induced the apoptosis of HT‑29 cancer cells in an Annexin V and PI double staining assay. Moreover, treatment with GA significantly decreased miR‑21 expression in these cells. Additionally, western blot analysis demonstrated that GA treatment enhanced the activation of phosphatase and tensin homolog (PTEN) along with the suppression of PI3K and p‑Akt. Furthermore, miR‑21 mimics reversed all the aforementioned activities of GA, which indicated that miR‑21 was the effector of GA and blocked PI3K/Akt signaling pathway via enhancing PTEN activity. In summary, GA induced HT‑29 cancer cell apoptosis via decreasing miR‑21 expression and blocking PI3K/Akt, which may be a useful novel insight for future CRC treatment.
View Figures
View References
Journal Cover

September-2018
Volume 16 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Gao G, Bian Y, Qian H, Yang M, Hu J, Li L, Yu L, Liu B and Qian X: Gambogic acid regulates the migration and invasion of colorectal cancer via microRNA‑21‑mediated activation of phosphatase and tensin homolog. Exp Ther Med 16: 1758-1765, 2018.
APA
Gao, G., Bian, Y., Qian, H., Yang, M., Hu, J., Li, L. ... Qian, X. (2018). Gambogic acid regulates the migration and invasion of colorectal cancer via microRNA‑21‑mediated activation of phosphatase and tensin homolog. Experimental and Therapeutic Medicine, 16, 1758-1765. https://doi.org/10.3892/etm.2018.6421
MLA
Gao, G., Bian, Y., Qian, H., Yang, M., Hu, J., Li, L., Yu, L., Liu, B., Qian, X."Gambogic acid regulates the migration and invasion of colorectal cancer via microRNA‑21‑mediated activation of phosphatase and tensin homolog". Experimental and Therapeutic Medicine 16.3 (2018): 1758-1765.
Chicago
Gao, G., Bian, Y., Qian, H., Yang, M., Hu, J., Li, L., Yu, L., Liu, B., Qian, X."Gambogic acid regulates the migration and invasion of colorectal cancer via microRNA‑21‑mediated activation of phosphatase and tensin homolog". Experimental and Therapeutic Medicine 16, no. 3 (2018): 1758-1765. https://doi.org/10.3892/etm.2018.6421
a