Comprehensive analysis of gene expression profiles associated with proliferative diabetic retinopathy

Gong,Meng‑Ting; Li,Wen‑Xing; Zhang,Qing; Lv,Wen‑Wen; He,Zheng‑Hong; Zhou,Shu‑Li; Zhang,Hui; Wang,Jing; He,Kan

doi:10.3892/etm.2018.6635

Comprehensive analysis of gene expression profiles associated with proliferative diabetic retinopathy

Authors:
- Meng‑Ting Gong
- Wen‑Xing Li
- Qing Zhang
- Wen‑Wen Lv
- Zheng‑Hong He
- Shu‑Li Zhou
- Hui Zhang
- Jing Wang
- Kan He
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Affiliations: Center for Stem Cell and Translational Medicine, School of Life Sciences, Anhui University, Hefei, Anhui 230601, P.R. China, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P.R. China, Department of Ophthalmology, The Second Hospital of Anhui Medical University, Hefei, Anhui 230601, P.R. China, Hongqiao International Institute of Medicine, Shanghai Tongren Hospital/Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China, Department of Biostatistics, School of Life Sciences, Anhui University, Hefei, Anhui 230601, P.R. China
Published online on: August 21, 2018 https://doi.org/10.3892/etm.2018.6635
Pages: 3539-3545
Copyright: © Gong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Proliferative diabetic retinopathy (PDR) is characterized by neovascularization on the surface of the retina or the optic disc, which is associated with environmental and genetic factors. However, its regulatory mechanism remains to be fully elucidated, particularly at a multiomics level. In the present study, a comprehensive analysis was performed of the gene expression profile of fibrovascular membranes (FVMs) associated with PDR, including an analysis of differentially expressed genes, functional enrichment, and regulation of transcription factors (TFs). As a result, novel marker genes of PDR were identified, including flavin containing monooxygenase 2. Furthermore, several common or specific genes, pathways and TFs have been recovered for active and inactive FVMs. In the present study, lymphoid enhancer binding factor 1 (LEF1) was identified as an upregulator in active and inactive FVMs, which is capable of activating or repressing target genes, including claudin 2, secreted phosphoprotein 1 (SPP1), and aristaless‑like homeobox 4. It was demonstrated that the Wnt/β‑catenin effector LEF1 regulating SPP1 is potentially important in PDR. The results of the present study may provide novel insights into the molecular mechanisms underlying the pathophysiology of PDR.

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