Open Access

Identification of mundoserone by zebrafish in vivo screening as a natural product with anti‑angiogenic activity

  • Authors:
    • Kan Chen
    • Changqian Wang
    • Yuqi Fan
    • Jun Gu
    • Zhihua Han
    • Yue Wang
    • Lin Gao
    • Huasu Zeng
  • View Affiliations

  • Published online on: September 17, 2018     https://doi.org/10.3892/etm.2018.6748
  • Pages: 4562-4568
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to screen natural products with anti‑angiogenic potential from the Natural Products Collection of MicroSource. The anti‑angiogenic activity of 240 natural products was assessed using the zebrafish line Tg(fli1a: EGFP)y1. At 24 h post‑fertilization, the embryos were treated with the library compounds for 24 h and, the morphology of the intersegmental vessels (ISVs) was then assessed using a fluorescence microscope, followed by counting of ISVs and calculation of the inhibition ratio. The expression of angiogenesis‑associated genes was determined by quantitative polymerase chain reaction. The results indicated that mundoserone inhibited ISV formation in zebrafish embryos in a dose‑dependent manner, with a significant anti‑angiogenic activity observed at a concentration of 10 µM, leading to an ISV inhibition ratio of 73.6±1.3%. Mundoserone significantly reduced the expression of slit guidance ligand 3 (SLIT3), roundabout guidance receptor 1 (ROBO1) and ‑2, fibroblast growth factor receptor (FGFR)2 and ‑3, as well as protein tyrosine phosphatase, receptor type B (PTP‑RB), but increased the expression of NOTCH1A. Accordingly, mundoserone may be an effective angiogenic inhibitor, which acts via downregulation of SLIT/ROBO1 and FGFR/PTP‑RB, and upregulation of NOTCH1A signaling.
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December-2018
Volume 16 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chen K, Wang C, Fan Y, Gu J, Han Z, Wang Y, Gao L and Zeng H: Identification of mundoserone by zebrafish in vivo screening as a natural product with anti‑angiogenic activity. Exp Ther Med 16: 4562-4568, 2018.
APA
Chen, K., Wang, C., Fan, Y., Gu, J., Han, Z., Wang, Y. ... Zeng, H. (2018). Identification of mundoserone by zebrafish in vivo screening as a natural product with anti‑angiogenic activity. Experimental and Therapeutic Medicine, 16, 4562-4568. https://doi.org/10.3892/etm.2018.6748
MLA
Chen, K., Wang, C., Fan, Y., Gu, J., Han, Z., Wang, Y., Gao, L., Zeng, H."Identification of mundoserone by zebrafish in vivo screening as a natural product with anti‑angiogenic activity". Experimental and Therapeutic Medicine 16.6 (2018): 4562-4568.
Chicago
Chen, K., Wang, C., Fan, Y., Gu, J., Han, Z., Wang, Y., Gao, L., Zeng, H."Identification of mundoserone by zebrafish in vivo screening as a natural product with anti‑angiogenic activity". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4562-4568. https://doi.org/10.3892/etm.2018.6748