Osteogenic efficiency in vivo of scaffolding material of prefabricated vascularization
- Authors:
- Xiaorui Jiang
- Huiying Yang
- Botao Huang
- Guodong Lin
- Kai Wang
- Yongchun Meng
- Chunyu Xue
View Affiliations
Affiliations: Department of Orthopedics, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China, Department of Hepatology, Yantai Infectious Disease Hospital, Yantai, Shandong 264000, P.R. China, Department of Orthopedics, The Affiliated Yantai Hospital of Binzhou Medical University, Yantai, Shandong 264000, P.R. China, Emergency Department, Qingdao Municipal Hospital, Qingdao, Shandong 266011, P.R. China
- Published online on: November 1, 2018 https://doi.org/10.3892/etm.2018.6915
-
Pages:
398-402
-
Copyright: © Jiang
et al. This is an open access article distributed under the
terms of Creative
Commons Attribution License.
Metrics: Total
Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
This article is mentioned in:
Abstract
Osteogenic efficiency of pre-vascularization and non-vascularization decalcified bone scaffolds in bone defect repair was investigated. Twenty-one Sprague‑Dawley (SD) mice were randomly assigned to three groups, and a bone defect area of ~1.5 cm in length in the thigh bone of the right posterior limbs of each mouse was made. Pre-vascularization decalcified bone scaffolds in vitro (group A) and non-treatment decalcified bone scaffolds (group B) were separately implanted. The defect vacancy was considered as the blank control (group C). Sampling was made 4 weeks after the operation for the histological examination, and then the osteogenic efficiency was observed by gross sample, imaging, hematoxylin and eosin staining and Masson's staining. When implanting pre-vascularization decalcified bone scaffolds in vitro, the scaffolding material showed an obvious absorption, and more new bone formations and abundant vascular proliferation were observed. In non-vascularization decalcified bone scaffolds implanting, absorption insufficiency of the scaffolding material was observed, fewer new-born bone formations were shown, and the new vessels were very small and few in number. The pre-vascularization decalcified bone scaffolds had a better osteogenic efficiency.
View References
|
1
|
Yousefi AM, James PF, Akbarzadeh R,
Subramanian A, Flavin C and Oudadesse H: Prospect of stem cells in
bone tissue engineering: A review. Stem Cells Int.
2016:61804872016. View Article : Google Scholar : PubMed/NCBI
|
|
2
|
Zhao T and Sun H: Insight into bone tissue
engineering scaffold materials and their vascularization. Zhongguo
Zu Zhi Gong Cheng Yan Jiu Yu Lin Chuang Kang Fu. 17:6832–6838.
2013.
|
|
3
|
Nakano K, Murata K, Omokawa S, Akahane M,
Shimizu T, Kawamura K, Kawate K and Tanaka Y: Promotion of
osteogenesis and angiogenesis in vascularized tissue-engineered
bone using osteogenic matrix cell sheets. Plast Reconstr Surg.
137:1476–1484. 2016. View Article : Google Scholar : PubMed/NCBI
|
|
4
|
Mercado-Pagán ÁE, Stahl AM, Shanjani Y and
Yang Y: Vascularization in bone tissue engineering constructs. Ann
Biomed Eng. 43:718–729. 2015. View Article : Google Scholar : PubMed/NCBI
|
|
5
|
Xu Z, Wang G and Wang F:
Histocompatibility of demineralized bone matrix decorated with
poly-L-lysine for scaffolds. Orthopedic J China. 16:1493–1497.
2015.
|
|
6
|
Amini AR, Laurencin CT and Nukavarapu SP:
Bone tissue engineering: Recent advances and challenges. Crit Rev
Biomed Eng. 40:363–408. 2012. View Article : Google Scholar : PubMed/NCBI
|
|
7
|
Zhou J, Lin H, Fang T, Li X, Dai W, Uemura
T and Dong J: The repair of large segmental bone defects in the
rabbit with vascularized tissue engineered bone. Biomaterials.
31:1171–1179. 2010. View Article : Google Scholar : PubMed/NCBI
|
|
8
|
Nguyen LH, Annabi N, Nikkhah M, Bae H,
Binan L, Park S, Kang Y, Yang Y and Khademhosseini A: Vascularized
bone tissue engineering: Approaches for potential improvement.
Tissue Eng Part B Rev. 18:363–382. 2012. View Article : Google Scholar : PubMed/NCBI
|
|
9
|
Almubarak S, Nethercott H, Freeberg M,
Beaudon C, Jha A, Jackson W, Marcucio R, Miclau T, Healy K and
Bahney C: Tissue engineering strategies for promoting vascularized
bone regeneration. Bone. 83:197–209. 2016. View Article : Google Scholar : PubMed/NCBI
|
|
10
|
Lovett M, Lee K, Edwards A and Kaplan DL:
Vascularization strategies for tissue engineering. Tissue Eng Part
B Rev. 15:353–370. 2009. View Article : Google Scholar : PubMed/NCBI
|
|
11
|
Bussolino F, Mantovani A and Persico G:
Molecular mechanisms of blood vessel formation. Trends Biochem Sci.
22:251–256. 1997. View Article : Google Scholar : PubMed/NCBI
|
|
12
|
Thottappillil N and Nair PD: Scaffolds in
vascular regeneration: Current status. Vasc Health Risk Manag.
11:79–91. 2015.PubMed/NCBI
|
