Determination of the chemical components and phospholipids of velvet antler using UPLC/QTOF‑MS coupled with UNIFI software

Zhang,Li‑Qun; Wang,Jia; Li,Ting; Li,Ping‑Ya; Wang,Yun‑Hua; Yang,Miao; Liu,Jin‑Ping; Liu,Ji‑Hua

doi:10.3892/etm.2019.7372

Determination of the chemical components and phospholipids of velvet antler using UPLC/QTOF‑MS coupled with UNIFI software

Authors:
- Li‑Qun Zhang
- Jia Wang
- Ting Li
- Ping‑Ya Li
- Yun‑Hua Wang
- Miao Yang
- Jin‑Ping Liu
- Ji‑Hua Liu
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Affiliations: Department of Obstetrics and Gynecology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Natural Medicinal Chemistry, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China
Published online on: March 12, 2019 https://doi.org/10.3892/etm.2019.7372
Pages: 3789-3799

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Abstract

Velvet antler, which exhibits immune and growth enhancing effects, is commonly used in a variety of Asian health care products, but its complex components remain unknown. The current study analyzed extracts using ultra‑performance liquid chromatography coupled with electrospray ionization quadrupole time‑of‑flight tandem mass spectrometry in the MSE mode. Automated detection and data filtering were performed using UNIFI software and peaks were compared with a proprietary scientific library (Traditional Medicine Library; TML). The results obtained using different data processing parameters (including 3D peak detection, target by mass and fragment identification) were evaluated against 87 compounds comprising 1 lignan, 30 terpenoids (including 20 triterpenes), 39 steroids, 8 alkaloids, 4 organic acids and 5 esters in the TML. Using a screening method with a mass accuracy cutoff of ±2 mDa, a retention time cutoff of ±0.2 min, a minimum response threshold of 1,000 counts and an average of 10 false detects per sample analysis, 16 phospholipids were identified in the extracts of velvet antler, three of which were quantified. The results demonstrated that there was 1.07±0.02 µg/g of 1‑myristoyl‑sn‑glycero‑3‑phosphocholine, 7.05±0.52 ng/g of 1,2‑dimyristoyl‑sn‑glycero‑3‑phosphocholine and 18.81±0.55 ng/g of 1‑palmitoyl‑2‑oleoyl‑sn‑glycero‑3‑phosphocholine in velvet antler. The current study successfully identified certain components of velvet antler. Furthermore, the results may provide an experimental basis for further pharmacological and clinical study.

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